Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000042                                                              18    1135
XP_006718907                                                           18    1135
XP_006718906                                                           18    1135
XP_005271619                                                           18    1135
XP_005271618                                                           18    1135
NP_055192                                                              17    2528
NP_001123359                                                           17    2528
XP_006720708                                                           17    2528
XP_006720707                                                           17    2528
NP_997716                                                              16    7355
NP_001130015                                                           16    7355
NP_001275648                                                           16    7355
NP_001275647                                                           16    7355
NP_001275649                                                           16    7355
NP_003143                                                              16    7355
NP_803879                                                              16    7355
NP_803881                                                              16    7355
NP_001258992                                                           16    7355
NP_110428                                                              16    7355
NP_001229291                                                           16    7355
NP_001258991                                                           16    7355
NP_001092270                                                           16    7355
NP_001258990                                                           16    7355
XP_006726908                                                           16    7355
XP_005276770                                                           16    7355
XP_006724671                                                           16    7355
XP_006724670                                                           16    7355
XP_005257681                                                           16    7355
NP_000731                                                              15    9605
NP_001107578                                                           15    9605
NP_001245271                                                           15    9605
NP_001245269                                                           15    9605
NP_689582                                                              15    9605
NP_001245270                                                           15    9605
NP_001245272                                                           15    9605
NP_001077362                                                           15    9605
XP_005273091                                                           15    9605
XP_005273090                                                           15    9605
XP_006711795                                                           15    9605
XP_005273089                                                           15    9605
XP_005270500                                                           15    9605
XP_005270499                                                           15    9605
XP_005270498                                                           15    9605
XP_005270497                                                           15    9605
>NP_000042
          Length = 3056

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FIKRANRRA 9
           FIK ANRRA
Sbjct: 104 FIKCANRRA 112
>XP_006718907
          Length = 3050

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FIKRANRRA 9
           FIK ANRRA
Sbjct: 104 FIKCANRRA 112
>XP_006718906
          Length = 3056

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FIKRANRRA 9
           FIK ANRRA
Sbjct: 104 FIKCANRRA 112
>XP_005271619
          Length = 3056

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FIKRANRRA 9
           FIK ANRRA
Sbjct: 104 FIKCANRRA 112
>XP_005271618
          Length = 3056

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FIKRANRRA 9
           FIK ANRRA
Sbjct: 104 FIKCANRRA 112
>NP_055192
          Length = 621

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  FIKRANRRA 9
          F+KR NR A
Sbjct: 38 FVKRRNRNA 46
>NP_001123359
          Length = 568

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  FIKRANRRA 9
          F+KR NR A
Sbjct: 38 FVKRRNRNA 46
>XP_006720708
          Length = 367

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  IKRANRRA 9
          ++RANRRA
Sbjct: 63 VQRANRRA 70
>XP_006720707
          Length = 445

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  IKRANRRA 9
          ++RANRRA
Sbjct: 63 VQRANRRA 70
>NP_997716
          Length = 227

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  IKRANR 7
          IKRANR
Sbjct: 64 IKRANR 69
>NP_001130015
          Length = 179

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  IKRANR 7
          IKRANR
Sbjct: 16 IKRANR 21
>NP_001275648
          Length = 764

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   IKRANRR 8
           IKRA+RR
Sbjct: 394 IKRADRR 400
>NP_001275647
          Length = 794

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   IKRANRR 8
           IKRA+RR
Sbjct: 424 IKRADRR 430
>NP_001275649
          Length = 763

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   IKRANRR 8
           IKRA+RR
Sbjct: 424 IKRADRR 430
>NP_003143
          Length = 794

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   IKRANRR 8
           IKRA+RR
Sbjct: 424 IKRADRR 430
>NP_803879
          Length = 741

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   RANRRA 9
           RANRRA
Sbjct: 689 RANRRA 694
>NP_803881
          Length = 739

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   RANRRA 9
           RANRRA
Sbjct: 689 RANRRA 694
>NP_001258992
          Length = 741

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   RANRRA 9
           RANRRA
Sbjct: 689 RANRRA 694
>NP_110428
          Length = 741

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   RANRRA 9
           RANRRA
Sbjct: 689 RANRRA 694
>NP_001229291
          Length = 757

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   RANRRA 9
           RANRRA
Sbjct: 705 RANRRA 710
>NP_001258991
          Length = 741

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   RANRRA 9
           RANRRA
Sbjct: 689 RANRRA 694
>NP_001092270
          Length = 739

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   RANRRA 9
           RANRRA
Sbjct: 689 RANRRA 694
>NP_001258990
          Length = 757

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   RANRRA 9
           RANRRA
Sbjct: 705 RANRRA 710
>XP_006726908
          Length = 185

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   RANRRA 9
           RANRRA
Sbjct: 135 RANRRA 140
>XP_005276770
          Length = 187

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   RANRRA 9
           RANRRA
Sbjct: 135 RANRRA 140
>XP_006724671
          Length = 741

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   RANRRA 9
           RANRRA
Sbjct: 689 RANRRA 694
>XP_006724670
          Length = 741

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   RANRRA 9
           RANRRA
Sbjct: 689 RANRRA 694
>XP_005257681
          Length = 761

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   IKRANRR 8
           IKRA+RR
Sbjct: 424 IKRADRR 430
>NP_000731
          Length = 590

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   IKRANRR 8
           +KR+NRR
Sbjct: 300 MKRSNRR 306
>NP_001107578
          Length = 653

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   FIKRANR 7
           F+KR+N+
Sbjct: 621 FVKRSNK 627
>NP_001245271
          Length = 617

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   FIKRANR 7
           F+KR+N+
Sbjct: 585 FVKRSNK 591
>NP_001245269
          Length = 652

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   FIKRANR 7
           F+KR+N+
Sbjct: 620 FVKRSNK 626
>NP_689582
          Length = 605

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   FIKRANR 7
           F+KR+N+
Sbjct: 573 FVKRSNK 579
>NP_001245270
          Length = 620

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   FIKRANR 7
           F+KR+N+
Sbjct: 588 FVKRSNK 594
>NP_001245272
          Length = 585

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   FIKRANR 7
           F+KR+N+
Sbjct: 553 FVKRSNK 559
>NP_001077362
          Length = 797

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   IKRANR 7
           +KRANR
Sbjct: 365 VKRANR 370
>XP_005273091
          Length = 590

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   IKRANRR 8
           +KR+NRR
Sbjct: 300 MKRSNRR 306
>XP_005273090
          Length = 590

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   IKRANRR 8
           +KR+NRR
Sbjct: 300 MKRSNRR 306
>XP_006711795
          Length = 590

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   IKRANRR 8
           +KR+NRR
Sbjct: 300 MKRSNRR 306
>XP_005273089
          Length = 590

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   IKRANRR 8
           +KR+NRR
Sbjct: 300 MKRSNRR 306
>XP_005270500
          Length = 573

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   FIKRANR 7
           F+KR+N+
Sbjct: 541 FVKRSNK 547
>XP_005270499
          Length = 574

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   FIKRANR 7
           F+KR+N+
Sbjct: 542 FVKRSNK 548
>XP_005270498
          Length = 605

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   FIKRANR 7
           F+KR+N+
Sbjct: 573 FVKRSNK 579
>XP_005270497
          Length = 621

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   FIKRANR 7
           F+KR+N+
Sbjct: 589 FVKRSNK 595
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.343    0.142    0.395 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 346,018
Number of extensions: 530
Number of successful extensions: 44
Number of sequences better than 10000.0: 44
Number of HSP's gapped: 44
Number of HSP's successfully gapped: 44
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.7 bits)
S2: 28 (15.4 bits)

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