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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_733751                                                              18    1935
XP_006716142                                                           18    1935
XP_005250088                                                           18    1935
XP_006716141                                                           18    1935
XP_005250085                                                           18    1935
XP_006716140                                                           18    1935
XP_005250084                                                           18    1935
XP_005250083                                                           18    1935
XP_005250082                                                           18    1935
NP_694551                                                              16    5631
NP_001122099                                                           16    5631
NP_001122098                                                           16    5631
NP_064533                                                              16    5631
XP_006722397                                                           16    5631
XP_005258179                                                           16    5631
XP_005258178                                                           16    5631
XP_006722396                                                           16    5631
NP_002372                                                              16    7355
NP_002371                                                              16    7355
NP_085072                                                              16    7355
XP_005250977                                                           16    7355
NP_001019820                                                           15    9605
NP_001737                                                              15    9605
NP_055616                                                              15    9605
NP_085095                                                              15    9605
NP_085080                                                              15    9605
NP_003824                                                              15    9605
XP_005260654                                                           15    9605
XP_006711879                                                           15    9605
XP_005273329                                                           15    9605

>NP_733751
          Length = 4911

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    IISSSRRI 9
            IISSSRRI
Sbjct: 4869 IISSSRRI 4876



>XP_006716142
          Length = 4927

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    IISSSRRI 9
            IISSSRRI
Sbjct: 4885 IISSSRRI 4892



>XP_005250088
          Length = 4928

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    IISSSRRI 9
            IISSSRRI
Sbjct: 4886 IISSSRRI 4893



>XP_006716141
          Length = 4959

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    IISSSRRI 9
            IISSSRRI
Sbjct: 4917 IISSSRRI 4924



>XP_005250085
          Length = 4979

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    IISSSRRI 9
            IISSSRRI
Sbjct: 4937 IISSSRRI 4944



>XP_006716140
          Length = 4982

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    IISSSRRI 9
            IISSSRRI
Sbjct: 4940 IISSSRRI 4947



>XP_005250084
          Length = 4982

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    IISSSRRI 9
            IISSSRRI
Sbjct: 4940 IISSSRRI 4947



>XP_005250083
          Length = 4982

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    IISSSRRI 9
            IISSSRRI
Sbjct: 4940 IISSSRRI 4947



>XP_005250082
          Length = 4983

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    IISSSRRI 9
            IISSSRRI
Sbjct: 4941 IISSSRRI 4948



>NP_694551
          Length = 534

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   FIISSSRR 8
           F++SSSR+
Sbjct: 445 FVVSSSRQ 452



>NP_001122099
          Length = 622

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   IISSSRR 8
           IISSSRR
Sbjct: 578 IISSSRR 584



>NP_001122098
          Length = 756

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   IISSSRR 8
           IISSSRR
Sbjct: 712 IISSSRR 718



>NP_064533
          Length = 742

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   IISSSRR 8
           IISSSRR
Sbjct: 698 IISSSRR 704



>XP_006722397
          Length = 412

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   IISSSRR 8
           IISSSRR
Sbjct: 368 IISSSRR 374



>XP_005258179
          Length = 800

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   IISSSRR 8
           IISSSRR
Sbjct: 756 IISSSRR 762



>XP_005258178
          Length = 692

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   IISSSRR 8
           IISSSRR
Sbjct: 648 IISSSRR 654



>XP_006722396
          Length = 745

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   IISSSRR 8
           IISSSRR
Sbjct: 701 IISSSRR 707



>NP_002372
          Length = 486

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  FIISSSRRI 9
          FII SSR +
Sbjct: 86 FIIDSSRSV 94



>NP_002371
          Length = 956

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  FIISSSRRI 9
          FII SSR +
Sbjct: 60 FIIDSSRSV 68



>NP_085072
          Length = 937

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  FIISSSRRI 9
          FII SSR +
Sbjct: 60 FIIDSSRSV 68



>XP_005250977
          Length = 818

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  FIISSSRRI 9
          FII SSR +
Sbjct: 60 FIIDSSRSV 68



>NP_001019820
          Length = 592

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   FIISSSRRI 9
           FII + RRI
Sbjct: 438 FIICADRRI 446



>NP_001737
          Length = 592

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   FIISSSRRI 9
           FII + RRI
Sbjct: 438 FIICADRRI 446



>NP_055616
          Length = 2137

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    FIISSSRRI 9
            F+IS+ RR+
Sbjct: 1209 FLISNHRRL 1217



>NP_085095
          Length = 499

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  FIISSSRRI 9
          F+I SSR +
Sbjct: 37 FVIDSSRSV 45



>NP_085080
          Length = 540

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  FIISSSRRI 9
          F+I SSR +
Sbjct: 37 FVIDSSRSV 45



>NP_003824
          Length = 581

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  FIISSSRRI 9
          F+I SSR +
Sbjct: 37 FVIDSSRSV 45



>XP_005260654
          Length = 581

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  FIISSSRRI 9
          F+I SSR +
Sbjct: 37 FVIDSSRSV 45



>XP_006711879
          Length = 1364

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    FIISSSRRI 9
            F+IS+ RR+
Sbjct: 1209 FLISNHRRL 1217



>XP_005273329
          Length = 1934

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    FIISSSRRI 9
            F+IS+ RR+
Sbjct: 1006 FLISNHRRL 1014



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.343    0.147    0.359 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 344,576
Number of extensions: 304
Number of successful extensions: 30
Number of sequences better than 10000.0: 30
Number of HSP's gapped: 30
Number of HSP's successfully gapped: 30
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.6 bits)
S2: 28 (15.4 bits)