Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000213                                                              20     390
NP_001087241                                                           20     390
XP_006714101                                                           20     390
XP_005265799                                                           20     390
XP_005265798                                                           20     390
XP_005265797                                                           20     390
NP_001124463                                                           17    3301
NP_006380                                                              17    3301
XP_005271451                                                           17    3301
XP_005271450                                                           17    3301
XP_005271449                                                           17    3301
XP_005271447                                                           17    3301
NP_066278                                                              17    4312
NP_001258662                                                           17    4312
XP_006713232                                                           17    4312
XP_006713230                                                           17    4312
NP_001273171                                                           16    5631
NP_001511                                                              16    5631
XP_006721105                                                           16    5631
NP_056224                                                              16    7355
NP_004487                                                              16    7355
NP_115495                                                              15    9605
NP_003195                                                              15    9605
NP_000731                                                              15    9605
NP_001008537                                                           15    9605
XP_006724709                                                           15    9605
XP_006724708                                                           15    9605
XP_005257472                                                           15    9605
XP_005257470                                                           15    9605
XP_005257469                                                           15    9605
XP_005257468                                                           15    9605
XP_005257467                                                           15    9605
XP_005273091                                                           15    9605
XP_005273090                                                           15    9605
XP_006711795                                                           15    9605
XP_005273089                                                           15    9605
>NP_000213
          Length = 976

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   FGSANVTT 8
           FGSANVTT
Sbjct: 296 FGSANVTT 303
>NP_001087241
          Length = 972

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   FGSANVTT 8
           FGSANVTT
Sbjct: 296 FGSANVTT 303
>XP_006714101
          Length = 526

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   FGSANVTT 8
           FGSANVTT
Sbjct: 297 FGSANVTT 304
>XP_005265799
          Length = 973

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   FGSANVTT 8
           FGSANVTT
Sbjct: 297 FGSANVTT 304
>XP_005265798
          Length = 976

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   FGSANVTT 8
           FGSANVTT
Sbjct: 297 FGSANVTT 304
>XP_005265797
          Length = 977

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   FGSANVTT 8
           FGSANVTT
Sbjct: 297 FGSANVTT 304
>NP_001124463
          Length = 999

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FGSANVTT 8
           FGS N+TT
Sbjct: 511 FGSQNLTT 518
>NP_006380
          Length = 999

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FGSANVTT 8
           FGS N+TT
Sbjct: 511 FGSQNLTT 518
>XP_005271451
          Length = 1000

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FGSANVTT 8
           FGS N+TT
Sbjct: 511 FGSQNLTT 518
>XP_005271450
          Length = 1000

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FGSANVTT 8
           FGS N+TT
Sbjct: 511 FGSQNLTT 518
>XP_005271449
          Length = 1000

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FGSANVTT 8
           FGS N+TT
Sbjct: 511 FGSQNLTT 518
>XP_005271447
          Length = 1000

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FGSANVTT 8
           FGS N+TT
Sbjct: 511 FGSQNLTT 518
>NP_066278
          Length = 725

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   GSANVTTA 9
           G+AN TTA
Sbjct: 307 GTANTTTA 314
>NP_001258662
          Length = 689

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   GSANVTTA 9
           G+AN TTA
Sbjct: 263 GTANTTTA 270
>XP_006713232
          Length = 519

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   GSANVTTA 9
           G+AN TTA
Sbjct: 307 GTANTTTA 314
>XP_006713230
          Length = 655

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   GSANVTTA 9
           G+AN TTA
Sbjct: 307 GTANTTTA 314
>NP_001273171
          Length = 2084

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    FGSANVTTA 9
            FG+AN++ A
Sbjct: 1938 FGAANISQA 1946
>NP_001511
          Length = 2109

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    FGSANVTTA 9
            FG+AN++ A
Sbjct: 1963 FGAANISQA 1971
>XP_006721105
          Length = 2046

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    FGSANVTTA 9
            FG+AN++ A
Sbjct: 1900 FGAANISQA 1908
>NP_056224
          Length = 3123

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    GSANVTTA 9
            GSA VTT+
Sbjct: 1035 GSARVTTS 1042
>NP_004487
          Length = 472

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   GSANVTT 8
           GSA+VTT
Sbjct: 440 GSASVTT 446
>NP_115495
          Length = 6306

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1    FGSANVT 7
            +G+AN+T
Sbjct: 5612 YGTANIT 5618
>NP_003195
          Length = 772

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   FGSANVT 7
           FGS N+T
Sbjct: 408 FGSTNLT 414
>NP_000731
          Length = 590

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  FGSANVTTA 9
          FGS NV+ A
Sbjct: 37 FGSYNVSRA 45
>NP_001008537
          Length = 1516

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   SANVTT 8
           SANVTT
Sbjct: 800 SANVTT 805
>XP_006724709
          Length = 1486

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   SANVTT 8
           SANVTT
Sbjct: 800 SANVTT 805
>XP_006724708
          Length = 1486

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   SANVTT 8
           SANVTT
Sbjct: 800 SANVTT 805
>XP_005257472
          Length = 731

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   FGSANVT 7
           FGS N+T
Sbjct: 367 FGSTNLT 373
>XP_005257470
          Length = 742

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   FGSANVT 7
           FGS N+T
Sbjct: 378 FGSTNLT 384
>XP_005257469
          Length = 742

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   FGSANVT 7
           FGS N+T
Sbjct: 378 FGSTNLT 384
>XP_005257468
          Length = 761

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   FGSANVT 7
           FGS N+T
Sbjct: 397 FGSTNLT 403
>XP_005257467
          Length = 772

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   FGSANVT 7
           FGS N+T
Sbjct: 408 FGSTNLT 414
>XP_005273091
          Length = 590

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  FGSANVTTA 9
          FGS NV+ A
Sbjct: 37 FGSYNVSRA 45
>XP_005273090
          Length = 590

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  FGSANVTTA 9
          FGS NV+ A
Sbjct: 37 FGSYNVSRA 45
>XP_006711795
          Length = 590

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  FGSANVTTA 9
          FGS NV+ A
Sbjct: 37 FGSYNVSRA 45
>XP_005273089
          Length = 590

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  FGSANVTTA 9
          FGS NV+ A
Sbjct: 37 FGSYNVSRA 45
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.309    0.115    0.308 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 337,003
Number of extensions: 531
Number of successful extensions: 36
Number of sequences better than 10000.0: 36
Number of HSP's gapped: 36
Number of HSP's successfully gapped: 36
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)

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