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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004324                                                              19     869
NP_002871                                                              19     869
XP_005250103                                                           19     869
XP_005250102                                                           19     869
XP_005265416                                                           19     869
XP_005265415                                                           19     869
XP_005265414                                                           19     869
XP_005265412                                                           19     869
NP_001243125                                                           18    1935
NP_001645                                                              18    1935
XP_006724592                                                           18    1935
NP_005021                                                              16    5631
NP_001186916                                                           16    7355
NP_002484                                                              16    7355
XP_005252323                                                           16    7355
XP_005252322                                                           16    7355
XP_005252321                                                           16    7355
XP_005252320                                                           16    7355
NP_001092272                                                           16    7355
NP_115547                                                              16    7355
NP_872387                                                              15    9605
NP_001018081                                                           15    9605
NP_001623                                                              15    9605
NP_078879                                                              15    9605
NP_000709                                                              15    9605
NP_001230741                                                           15    9605
XP_005246496                                                           15    9605
XP_005245160                                                           15    9605
XP_006710960                                                           15    9605
XP_005271256                                                           15    9605
XP_006710959                                                           15    9605
XP_006710958                                                           15    9605
NP_112603                                                              15    9605

>NP_004324
          Length = 766

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FGLATKKSR 9
           FGLAT KSR
Sbjct: 595 FGLATVKSR 603



>NP_002871
          Length = 648

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FGLATKKSR 9
           FGLAT KSR
Sbjct: 487 FGLATVKSR 495



>XP_005250103
          Length = 758

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FGLATKKSR 9
           FGLAT KSR
Sbjct: 595 FGLATVKSR 603



>XP_005250102
          Length = 767

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FGLATKKSR 9
           FGLAT KSR
Sbjct: 595 FGLATVKSR 603



>XP_005265416
          Length = 534

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FGLATKKSR 9
           FGLAT KSR
Sbjct: 373 FGLATVKSR 381



>XP_005265415
          Length = 567

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FGLATKKSR 9
           FGLAT KSR
Sbjct: 406 FGLATVKSR 414



>XP_005265414
          Length = 615

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FGLATKKSR 9
           FGLAT KSR
Sbjct: 454 FGLATVKSR 462



>XP_005265412
          Length = 648

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FGLATKKSR 9
           FGLAT KSR
Sbjct: 487 FGLATVKSR 495



>NP_001243125
          Length = 609

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   FGLATKKSR 9
           FGLAT K+R
Sbjct: 451 FGLATVKTR 459



>NP_001645
          Length = 606

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   FGLATKKSR 9
           FGLAT K+R
Sbjct: 448 FGLATVKTR 456



>XP_006724592
          Length = 611

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   FGLATKKSR 9
           FGLAT K+R
Sbjct: 453 FGLATVKTR 461



>NP_005021
          Length = 603

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   FGLATK 6
           FGLATK
Sbjct: 195 FGLATK 200



>NP_001186916
          Length = 97

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  FGLATKKSR 9
          +G+  KKSR
Sbjct: 64 YGIVEKKSR 72



>NP_002484
          Length = 128

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FGLATKKSR 9
           +G+  KKSR
Sbjct: 95  YGIVEKKSR 103



>XP_005252323
          Length = 635

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   GLATKK 7
           GLATKK
Sbjct: 271 GLATKK 276



>XP_005252322
          Length = 690

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   GLATKK 7
           GLATKK
Sbjct: 358 GLATKK 363



>XP_005252321
          Length = 696

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   GLATKK 7
           GLATKK
Sbjct: 358 GLATKK 363



>XP_005252320
          Length = 706

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   GLATKK 7
           GLATKK
Sbjct: 358 GLATKK 363



>NP_001092272
          Length = 722

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   GLATKK 7
           GLATKK
Sbjct: 358 GLATKK 363



>NP_115547
          Length = 706

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   GLATKK 7
           GLATKK
Sbjct: 358 GLATKK 363



>NP_872387
          Length = 261

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   ATKKSR 9
           ATKKSR
Sbjct: 153 ATKKSR 158



>NP_001018081
          Length = 1309

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   FGLATKKSR 9
           FGLA  KS+
Sbjct: 383 FGLAYMKSK 391



>NP_001623
          Length = 535

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   FGLATKKSR 9
           FGLA  K+R
Sbjct: 397 FGLAPGKAR 405



>NP_078879
          Length = 421

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   GLATKKSR 9
           G+ATKK +
Sbjct: 345 GVATKKKK 352



>NP_000709
          Length = 2339

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   ATKKSR 9
           ATKKSR
Sbjct: 420 ATKKSR 425



>NP_001230741
          Length = 2237

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   ATKKSR 9
           ATKKSR
Sbjct: 420 ATKKSR 425



>XP_005246496
          Length = 550

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   FGLATKKSR 9
           FGLA  K+R
Sbjct: 412 FGLAPGKAR 420



>XP_005245160
          Length = 223

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   ATKKSR 9
           ATKKSR
Sbjct: 115 ATKKSR 120



>XP_006710960
          Length = 252

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   GLATKKSR 9
           G+ATKK +
Sbjct: 176 GVATKKKK 183



>XP_005271256
          Length = 252

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   GLATKKSR 9
           G+ATKK +
Sbjct: 176 GVATKKKK 183



>XP_006710959
          Length = 336

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   GLATKKSR 9
           G+ATKK +
Sbjct: 260 GVATKKKK 267



>XP_006710958
          Length = 367

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   GLATKKSR 9
           G+ATKK +
Sbjct: 291 GVATKKKK 298



>NP_112603
          Length = 532

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   FGLATKKSR 9
           FGLA  K+R
Sbjct: 394 FGLAPGKAR 402



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.320    0.131    0.350 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 375,329
Number of extensions: 428
Number of successful extensions: 33
Number of sequences better than 10000.0: 33
Number of HSP's gapped: 33
Number of HSP's successfully gapped: 33
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)