Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005219                                                              20     509
NP_000206                                                              17    2528
XP_006722808                                                           17    2528
XP_005259953                                                           17    2528
NP_036221                                                              17    4312
NP_078966                                                              16    5631
NP_064517                                                              16    5631
XP_006724094                                                           16    5631
XP_006724093                                                           16    5631
XP_006724092                                                           16    5631
XP_005261217                                                           16    5631
XP_005261216                                                           16    5631
XP_006723004                                                           16    5631
NP_001032642                                                           16    5631
NP_006720                                                              16    7355
NP_003730                                                              16    7355
NP_001240837                                                           16    7355
NP_009293                                                              16    7355
NP_995317                                                              16    7355
NP_001345                                                              16    7355
NP_005319                                                              16    7355
NP_055855                                                              16    7355
NP_004068                                                              16    7355
NP_001809                                                              16    7355
NP_004769                                                              16    7355
NP_001094891                                                           16    7355
XP_005272028                                                           16    7355
XP_005272027                                                           16    7355
XP_005272026                                                           16    7355
XP_005272025                                                           16    7355
XP_005272024                                                           16    7355
XP_006712425                                                           16    7355
XP_006712424                                                           16    7355
NP_001344                                                              16    7355
NP_001017372                                                           16    7355
NP_054750                                                              16    7355
NP_055078                                                              15    9605
NP_783163                                                              15    9605
NP_055099                                                              15    9605
NP_001268366                                                           15    9605
NP_001014839                                                           15    9605
NP_065075                                                              15    9605
NP_647473                                                              15    9605
NP_001240795                                                           15    9605
NP_001013663                                                           15    9605
NP_001268367                                                           15    9605
NP_008933                                                              15    9605
XP_005276449                                                           15    9605
XP_005260314                                                           15    9605
XP_005246746                                                           15    9605
NP_001120729                                                           15    9605
NP_001014841                                                           15    9605
>NP_005219
          Length = 1210

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   FGLAKLLG 8
           FGLAKLLG
Sbjct: 856 FGLAKLLG 863
>NP_000206
          Length = 1124

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   FGLAKLL 7
           FGLAKLL
Sbjct: 968 FGLAKLL 974
>XP_006722808
          Length = 1124

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   FGLAKLL 7
           FGLAKLL
Sbjct: 968 FGLAKLL 974
>XP_005259953
          Length = 1167

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1    FGLAKLL 7
            FGLAKLL
Sbjct: 1011 FGLAKLL 1017
>NP_036221
          Length = 738

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  GLAKLLGV 9
          GLA+LLG+
Sbjct: 85 GLARLLGL 92
>NP_078966
          Length = 14507

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2     GLAKLLGV 9
             GLA LLGV
Sbjct: 14459 GLAGLLGV 14466
>NP_064517
          Length = 376

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   FGLAKLLGV 9
           FG+ +L+GV
Sbjct: 350 FGVRRLIGV 358
>XP_006724094
          Length = 314

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   FGLAKLLGV 9
           FG+ +L+GV
Sbjct: 288 FGVRRLIGV 296
>XP_006724093
          Length = 376

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   FGLAKLLGV 9
           FG+ +L+GV
Sbjct: 350 FGVRRLIGV 358
>XP_006724092
          Length = 376

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   FGLAKLLGV 9
           FG+ +L+GV
Sbjct: 350 FGVRRLIGV 358
>XP_005261217
          Length = 376

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   FGLAKLLGV 9
           FG+ +L+GV
Sbjct: 350 FGVRRLIGV 358
>XP_005261216
          Length = 463

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   FGLAKLLGV 9
           FG+ +L+GV
Sbjct: 437 FGVRRLIGV 445
>XP_006723004
          Length = 6443

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    GLAKLLGV 9
            GLA LLGV
Sbjct: 6395 GLAGLLGV 6402
>NP_001032642
          Length = 376

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   FGLAKLLGV 9
           FG+ +L+GV
Sbjct: 350 FGVRRLIGV 358
>NP_006720
          Length = 1101

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   FGLAKLLG 8
           FGL ++LG
Sbjct: 245 FGLQRILG 252
>NP_003730
          Length = 323

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   GLAKLLGV 9
           GLAK +GV
Sbjct: 158 GLAKSIGV 165
>NP_001240837
          Length = 323

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   GLAKLLGV 9
           GLAK +GV
Sbjct: 158 GLAKSIGV 165
>NP_009293
          Length = 1096

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   FGLAKLLG 8
           FGL ++LG
Sbjct: 245 FGLQRILG 252
>NP_995317
          Length = 323

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   GLAKLLGV 9
           GLAK +GV
Sbjct: 158 GLAKSIGV 165
>NP_001345
          Length = 323

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   GLAKLLGV 9
           GLAK +GV
Sbjct: 158 GLAKSIGV 165
>NP_005319
          Length = 552

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  FGLAKLLGV 9
          FG++ LLG+
Sbjct: 18 FGVSLLLGI 26
>NP_055855
          Length = 2098

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    LAKLLGV 9
            LAK+LGV
Sbjct: 1910 LAKILGV 1916
>NP_004068
          Length = 466

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   FGLAKLLGV 9
           FG+++ LGV
Sbjct: 424 FGVSRALGV 432
>NP_001809
          Length = 323

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   GLAKLLGV 9
           GLAK +GV
Sbjct: 158 GLAKSIGV 165
>NP_004769
          Length = 395

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  GLAKLLGV 9
          GLA LLG+
Sbjct: 40 GLASLLGL 47
>NP_001094891
          Length = 1022

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LAKLLGV 9
           +AKLLGV
Sbjct: 303 IAKLLGV 309
>XP_005272028
          Length = 619

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   FGLAKL 6
           FGLAKL
Sbjct: 123 FGLAKL 128
>XP_005272027
          Length = 619

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   FGLAKL 6
           FGLAKL
Sbjct: 123 FGLAKL 128
>XP_005272026
          Length = 619

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   FGLAKL 6
           FGLAKL
Sbjct: 123 FGLAKL 128
>XP_005272025
          Length = 619

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   FGLAKL 6
           FGLAKL
Sbjct: 123 FGLAKL 128
>XP_005272024
          Length = 619

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   FGLAKL 6
           FGLAKL
Sbjct: 123 FGLAKL 128
>XP_006712425
          Length = 1349

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    LAKLLGV 9
            LAK+LGV
Sbjct: 1161 LAKILGV 1167
>XP_006712424
          Length = 1376

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    LAKLLGV 9
            LAK+LGV
Sbjct: 1188 LAKILGV 1194
>NP_001344
          Length = 323

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   GLAKLLGV 9
           GLAK +GV
Sbjct: 158 GLAKSIGV 165
>NP_001017372
          Length = 619

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   FGLAKL 6
           FGLAKL
Sbjct: 123 FGLAKL 128
>NP_054750
          Length = 619

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   FGLAKL 6
           FGLAKL
Sbjct: 123 FGLAKL 128
>NP_055078
          Length = 716

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   FGLAKLLGV 9
           FG+++ LGV
Sbjct: 635 FGMSEKLGV 643
>NP_783163
          Length = 407

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   GLAKLLG 8
           GLA LLG
Sbjct: 190 GLASLLG 196
>NP_055099
          Length = 729

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   GLAKLLG 8
           GLA++LG
Sbjct: 264 GLARILG 270
>NP_001268366
          Length = 368

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   GLAKLLG 8
           GLA LLG
Sbjct: 151 GLASLLG 157
>NP_001014839
          Length = 729

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   GLAKLLG 8
           GLA++LG
Sbjct: 264 GLARILG 270
>NP_065075
          Length = 831

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  FGLAKLL 7
          FGL KLL
Sbjct: 80 FGLEKLL 86
>NP_647473
          Length = 773

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   FGLAKLLGV 9
           FG+++ LGV
Sbjct: 692 FGMSEKLGV 700
>NP_001240795
          Length = 683

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   FGLAKLLGV 9
           FG+++ LGV
Sbjct: 602 FGMSEKLGV 610
>NP_001013663
          Length = 343

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   GLAKLLGV 9
           GLA LLG+
Sbjct: 158 GLATLLGL 165
>NP_001268367
          Length = 368

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   GLAKLLG 8
           GLA LLG
Sbjct: 151 GLASLLG 157
>NP_008933
          Length = 407

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   GLAKLLG 8
           GLA LLG
Sbjct: 190 GLASLLG 196
>XP_005276449
          Length = 171

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  GLAKLLG 8
          GLA++LG
Sbjct: 60 GLARMLG 66
>XP_005260314
          Length = 407

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   GLAKLLG 8
           GLA LLG
Sbjct: 190 GLASLLG 196
>XP_005246746
          Length = 898

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   FGLAKLL 7
           FGL KLL
Sbjct: 147 FGLEKLL 153
>NP_001120729
          Length = 831

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  FGLAKLL 7
          FGL KLL
Sbjct: 80 FGLEKLL 86
>NP_001014841
          Length = 712

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   GLAKLLG 8
           GLA++LG
Sbjct: 247 GLARILG 253
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.334    0.160    0.459 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 408,104
Number of extensions: 991
Number of successful extensions: 52
Number of sequences better than 10000.0: 52
Number of HSP's gapped: 52
Number of HSP's successfully gapped: 52
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)

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