Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              20     299
NP_001075109                                                           20     299
NP_000896                                                              18    1935
NP_065868                                                              18    1935
XP_006713778                                                           18    1935
NP_056082                                                              17    2528
NP_001189472                                                           17    2528
NP_853530                                                              17    2528
XP_006719362                                                           17    2528
XP_006715919                                                           17    2528
XP_006715917                                                           17    2528
XP_005250208                                                           17    2528
XP_005250207                                                           17    2528
XP_005264736                                                           17    4312
NP_955394                                                              16    5631
NP_001230823                                                           16    5631
NP_001230820                                                           16    5631
NP_001230824                                                           16    5631
NP_955393                                                              16    5631
NP_001230821                                                           16    5631
NP_003279                                                              16    5631
NP_955395                                                              16    5631
NP_955392                                                              16    5631
NP_955391                                                              16    5631
NP_998764                                                              16    7355
NP_001136121                                                           16    7355
NP_004259                                                              16    7355
XP_005252880                                                           16    7355
XP_005252879                                                           16    7355
XP_005252878                                                           16    7355
XP_005252877                                                           16    7355
XP_005266876                                                           16    7355
XP_006715392                                                           16    7355
XP_005266875                                                           16    7355
XP_006714901                                                           16    7355
NP_061063                                                              16    7355
NP_001092878                                                           16    7355
NP_008919                                                              15    9605
NP_061978                                                              15    9605
NP_062818                                                              15    9605
NP_112203                                                              15    9605
NP_001138396                                                           15    9605
NP_001244068                                                           15    9605
NP_000076                                                              15    9605
NP_001185982                                                           15    9605
NP_001136105                                                           15    9605
NP_055123                                                              15    9605
NP_149107                                                              15    9605
NP_002527                                                              15    9605
NP_001238784                                                           15    9605
NP_001238780                                                           15    9605
NP_001238781                                                           15    9605
NP_000373                                                              15    9605
NP_004061                                                              15    9605
NP_057594                                                              15    9605
NP_001238782                                                           15    9605
XP_005256637                                                           15    9605
XP_005256636                                                           15    9605
XP_005255663                                                           15    9605
XP_005255661                                                           15    9605
XP_005268266                                                           15    9605
XP_006720366                                                           15    9605
XP_005268265                                                           15    9605
XP_006714106                                                           15    9605
XP_005273123                                                           15    9605
XP_005273122                                                           15    9605
XP_005245790                                                           15    9605
XP_006710135                                                           15    9605
NP_001026976                                                           15    9605
NP_001036169                                                           15    9605
>NP_008835
          Length = 4128

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    FGKKRLGL 8
            FGKKRLGL
Sbjct: 2700 FGKKRLGL 2707
>NP_001075109
          Length = 4097

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    FGKKRLGL 8
            FGKKRLGL
Sbjct: 2700 FGKKRLGL 2707
>NP_000896
          Length = 97

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2  GKKRLGLS 9
          G KRLGLS
Sbjct: 3  GNKRLGLS 10
>NP_065868
          Length = 936

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  FGKKRLGLS 9
          F KKR+GL+
Sbjct: 10 FSKKRMGLN 18
>XP_006713778
          Length = 923

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  FGKKRLGLS 9
          F KKR+GL+
Sbjct: 10 FSKKRMGLN 18
>NP_056082
          Length = 1486

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   FGKKRLGLS 9
           FG+K LGLS
Sbjct: 919 FGEKVLGLS 927
>NP_001189472
          Length = 1516

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   FGKKRLGLS 9
           FG+K LGLS
Sbjct: 977 FGEKVLGLS 985
>NP_853530
          Length = 1505

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   FGKKRLGLS 9
           FG+K LGLS
Sbjct: 966 FGEKVLGLS 974
>XP_006719362
          Length = 1546

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   FGKKRLGLS 9
           FG+K LGLS
Sbjct: 979 FGEKVLGLS 987
>XP_006715919
          Length = 1494

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   FGKKRLGLS 9
           FG+K LGLS
Sbjct: 955 FGEKVLGLS 963
>XP_006715917
          Length = 1583

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    FGKKRLGLS 9
            FG+K LGLS
Sbjct: 1044 FGEKVLGLS 1052
>XP_005250208
          Length = 1603

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    FGKKRLGLS 9
            FG+K LGLS
Sbjct: 1064 FGEKVLGLS 1072
>XP_005250207
          Length = 1604

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    FGKKRLGLS 9
            FG+K LGLS
Sbjct: 1065 FGEKVLGLS 1073
>XP_005264736
          Length = 671

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  FGKKRLG 7
          F KKRLG
Sbjct: 21 FSKKRLG 27
>NP_955394
          Length = 220

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  KKRLGLS 9
          K+RLGLS
Sbjct: 78 KRRLGLS 84
>NP_001230823
          Length = 152

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  KKRLGLS 9
          K+RLGLS
Sbjct: 78 KRRLGLS 84
>NP_001230820
          Length = 181

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  KKRLGLS 9
          K+RLGLS
Sbjct: 53 KRRLGLS 59
>NP_001230824
          Length = 222

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  KKRLGLS 9
          K+RLGLS
Sbjct: 78 KRRLGLS 84
>NP_955393
          Length = 209

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  KKRLGLS 9
          K+RLGLS
Sbjct: 78 KRRLGLS 84
>NP_001230821
          Length = 163

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  KKRLGLS 9
          K+RLGLS
Sbjct: 55 KRRLGLS 61
>NP_003279
          Length = 206

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  KKRLGLS 9
          K+RLGLS
Sbjct: 78 KRRLGLS 84
>NP_955395
          Length = 200

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  KKRLGLS 9
          K+RLGLS
Sbjct: 78 KRRLGLS 84
>NP_955392
          Length = 229

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  KKRLGLS 9
          K+RLGLS
Sbjct: 78 KRRLGLS 84
>NP_955391
          Length = 186

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  KKRLGLS 9
          K+RLGLS
Sbjct: 78 KRRLGLS 84
>NP_998764
          Length = 913

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   GKKRLGL 8
           GKKR G+
Sbjct: 231 GKKRFGI 237
>NP_001136121
          Length = 912

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   GKKRLGL 8
           GKKR G+
Sbjct: 230 GKKRFGI 236
>NP_004259
          Length = 651

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   FGKKRLGLS 9
           FG KR+ LS
Sbjct: 400 FGHKRMRLS 408
>XP_005252880
          Length = 886

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   GKKRLGL 8
           GKKR G+
Sbjct: 204 GKKRFGI 210
>XP_005252879
          Length = 887

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   GKKRLGL 8
           GKKR G+
Sbjct: 205 GKKRFGI 211
>XP_005252878
          Length = 1072

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   GKKRLGL 8
           GKKR G+
Sbjct: 390 GKKRFGI 396
>XP_005252877
          Length = 1073

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   GKKRLGL 8
           GKKR G+
Sbjct: 391 GKKRFGI 397
>XP_005266876
          Length = 321

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KKRLGL 8
           KKRLGL
Sbjct: 196 KKRLGL 201
>XP_006715392
          Length = 325

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KKRLGL 8
           KKRLGL
Sbjct: 196 KKRLGL 201
>XP_005266875
          Length = 454

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KKRLGL 8
           KKRLGL
Sbjct: 196 KKRLGL 201
>XP_006714901
          Length = 242

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   KKRLGLS 9
           K+RLGLS
Sbjct: 209 KERLGLS 215
>NP_061063
          Length = 515

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KKRLGL 8
           KKRLGL
Sbjct: 196 KKRLGL 201
>NP_001092878
          Length = 242

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   KKRLGLS 9
           K+RLGLS
Sbjct: 209 KERLGLS 215
>NP_008919
          Length = 967

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1  FGKKRLG 7
          FG+++LG
Sbjct: 9  FGRRKLG 15
>NP_061978
          Length = 4242

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    GKKRLG 7
            G+KRLG
Sbjct: 3426 GRKRLG 3431
>NP_062818
          Length = 702

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   FGKKRLGLS 9
           FG+  LGLS
Sbjct: 621 FGRVYLGLS 629
>NP_112203
          Length = 774

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   FGKKRLGLS 9
           +G K++GL+
Sbjct: 180 YGSKKVGLT 188
>NP_001138396
          Length = 743

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   FGKKRLGLS 9
           +G K++GL+
Sbjct: 180 YGSKKVGLT 188
>NP_001244068
          Length = 644

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   FGKKRLGLS 9
           FG K +GLS
Sbjct: 101 FGAKVVGLS 109
>NP_000076
          Length = 687

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   FGKKRLGLS 9
           FG K +GLS
Sbjct: 144 FGAKVVGLS 152
>NP_001185982
          Length = 774

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   FGKKRLGLS 9
           +G K++GL+
Sbjct: 180 YGSKKVGLT 188
>NP_001136105
          Length = 880

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   GKKRLGL 8
           G KRLG+
Sbjct: 712 GSKRLGI 718
>NP_055123
          Length = 880

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   GKKRLGL 8
           G KRLG+
Sbjct: 712 GSKRLGI 718
>NP_149107
          Length = 979

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KKRLGLS 9
           KKRLG++
Sbjct: 635 KKRLGIN 641
>NP_002527
          Length = 688

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2  GKKRLGLS 9
          GKK  G+S
Sbjct: 78 GKKNFGIS 85
>NP_001238784
          Length = 494

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   GKKRLGL 8
           G KRLG+
Sbjct: 326 GSKRLGI 332
>NP_001238780
          Length = 494

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   GKKRLGL 8
           G KRLG+
Sbjct: 326 GSKRLGI 332
>NP_001238781
          Length = 494

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   GKKRLGL 8
           G KRLG+
Sbjct: 326 GSKRLGI 332
>NP_000373
          Length = 485

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   FGKKRLGL 8
           F K++LGL
Sbjct: 459 FNKEKLGL 466
>NP_004061
          Length = 687

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   FGKKRLGLS 9
           FG K +GLS
Sbjct: 144 FGAKVVGLS 152
>NP_057594
          Length = 453

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   FGKKRLGLS 9
           FG+KR+ L+
Sbjct: 346 FGQKRIHLN 354
>NP_001238782
          Length = 494

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   GKKRLGL 8
           G KRLG+
Sbjct: 326 GSKRLGI 332
>XP_005256637
          Length = 879

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   GKKRLGL 8
           G KRLG+
Sbjct: 711 GSKRLGI 717
>XP_005256636
          Length = 880

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   GKKRLGL 8
           G KRLG+
Sbjct: 712 GSKRLGI 718
>XP_005255663
          Length = 743

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   FGKKRLGLS 9
           +G K++GL+
Sbjct: 180 YGSKKVGLT 188
>XP_005255661
          Length = 774

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   FGKKRLGLS 9
           +G K++GL+
Sbjct: 180 YGSKKVGLT 188
>XP_005268266
          Length = 873

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KKRLGLS 9
           KKRLG++
Sbjct: 529 KKRLGIN 535
>XP_006720366
          Length = 971

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KKRLGLS 9
           KKRLG++
Sbjct: 647 KKRLGIN 653
>XP_005268265
          Length = 991

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KKRLGLS 9
           KKRLG++
Sbjct: 647 KKRLGIN 653
>XP_006714106
          Length = 478

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   FGKKRLGL 8
           FG++RL L
Sbjct: 461 FGQRRLSL 468
>XP_005273123
          Length = 432

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  GKKRLG 7
          G+KRLG
Sbjct: 28 GRKRLG 33
>XP_005273122
          Length = 464

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  GKKRLG 7
          G+KRLG
Sbjct: 28 GRKRLG 33
>XP_005245790
          Length = 686

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   FGKKRLGLS 9
           FG K +GLS
Sbjct: 144 FGAKVVGLS 152
>XP_006710135
          Length = 251

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   FGKKRLGLS 9
           FG K +GLS
Sbjct: 112 FGAKVVGLS 120
>NP_001026976
          Length = 508

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   FGKKRLGL 8
           F K++LGL
Sbjct: 459 FNKEKLGL 466
>NP_001036169
          Length = 686

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   FGKKRLGLS 9
           FG K +GLS
Sbjct: 144 FGAKVVGLS 152
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.325    0.150    0.427 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 659,088
Number of extensions: 1257
Number of successful extensions: 70
Number of sequences better than 10000.0: 70
Number of HSP's gapped: 70
Number of HSP's successfully gapped: 70
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)

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