Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001276866                                                           20     390
NP_004439                                                              20     390
NP_001276865                                                           20     390
NP_001005862                                                           20     390
XP_005257197                                                           20     390
XP_006721828                                                           20     390
NP_000143                                                              17    3301
NP_001073271                                                           17    3301
NP_001073272                                                           17    3301
XP_005257251                                                           17    3301
XP_005257250                                                           17    3301
NP_001268678                                                           17    4312
NP_079440                                                              17    4312
NP_001268677                                                           17    4312
XP_005271543                                                           17    4312
XP_005271542                                                           17    4312
XP_005271541                                                           17    4312
XP_005271539                                                           17    4312
XP_005271538                                                           17    4312
NP_001036189                                                           16    5631
XP_006710508                                                           16    5631
XP_006710507                                                           16    5631
NP_689910                                                              16    5631
NP_000284                                                              15    9605
NP_001027005                                                           15    9605
NP_001137432                                                           15    9605
NP_001275988                                                           15    9605
NP_001275990                                                           15    9605
NP_001275987                                                           15    9605
NP_112168                                                              15    9605
NP_001137431                                                           15    9605
XP_005256041                                                           15    9605
XP_005256040                                                           15    9605
XP_006718343                                                           15    9605
XP_006718342                                                           15    9605
XP_006718341                                                           15    9605
XP_005264347                                                           15    9605
XP_005264346                                                           15    9605
XP_005264345                                                           15    9605
XP_005264344                                                           15    9605
>NP_001276866
          Length = 1055

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   FGILIKRQQ 9
           FGILIKR+Q
Sbjct: 671 FGILIKRRQ 679
>NP_004439
          Length = 1255

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   FGILIKRQQ 9
           FGILIKR+Q
Sbjct: 671 FGILIKRRQ 679
>NP_001276865
          Length = 1240

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   FGILIKRQQ 9
           FGILIKR+Q
Sbjct: 656 FGILIKRRQ 664
>NP_001005862
          Length = 1225

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   FGILIKRQQ 9
           FGILIKR+Q
Sbjct: 641 FGILIKRRQ 649
>XP_005257197
          Length = 1225

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   FGILIKRQQ 9
           FGILIKR+Q
Sbjct: 641 FGILIKRRQ 649
>XP_006721828
          Length = 1336

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   FGILIKRQQ 9
           FGILIKR+Q
Sbjct: 752 FGILIKRRQ 760
>NP_000143
          Length = 952

 Score = 16.9 bits (32), Expect =  3301
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   FGILIKRQ 8
           FG++++RQ
Sbjct: 218 FGVIVRRQ 225
>NP_001073271
          Length = 952

 Score = 16.9 bits (32), Expect =  3301
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   FGILIKRQ 8
           FG++++RQ
Sbjct: 218 FGVIVRRQ 225
>NP_001073272
          Length = 952

 Score = 16.9 bits (32), Expect =  3301
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   FGILIKRQ 8
           FG++++RQ
Sbjct: 218 FGVIVRRQ 225
>XP_005257251
          Length = 952

 Score = 16.9 bits (32), Expect =  3301
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   FGILIKRQ 8
           FG++++RQ
Sbjct: 218 FGVIVRRQ 225
>XP_005257250
          Length = 952

 Score = 16.9 bits (32), Expect =  3301
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   FGILIKRQ 8
           FG++++RQ
Sbjct: 218 FGVIVRRQ 225
>NP_001268678
          Length = 1261

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   FGILIKRQQ 9
           F  LIKRQQ
Sbjct: 932 FAQLIKRQQ 940
>NP_079440
          Length = 1321

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    FGILIKRQQ 9
            F  LIKRQQ
Sbjct: 992  FAQLIKRQQ 1000
>NP_001268677
          Length = 1102

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   FGILIKRQQ 9
           F  LIKRQQ
Sbjct: 773 FAQLIKRQQ 781
>XP_005271543
          Length = 722

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   FGILIKRQQ 9
           F  LIKRQQ
Sbjct: 393 FAQLIKRQQ 401
>XP_005271542
          Length = 770

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   FGILIKRQQ 9
           F  LIKRQQ
Sbjct: 441 FAQLIKRQQ 449
>XP_005271541
          Length = 1203

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   FGILIKRQQ 9
           F  LIKRQQ
Sbjct: 874 FAQLIKRQQ 882
>XP_005271539
          Length = 1263

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   FGILIKRQQ 9
           F  LIKRQQ
Sbjct: 934 FAQLIKRQQ 942
>XP_005271538
          Length = 1369

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    FGILIKRQQ 9
            F  LIKRQQ
Sbjct: 1040 FAQLIKRQQ 1048
>NP_001036189
          Length = 538

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   GILIKRQQ 9
           GILI RQQ
Sbjct: 378 GILICRQQ 385
>XP_006710508
          Length = 677

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   GILIKRQ 8
           GILIK+Q
Sbjct: 663 GILIKKQ 669
>XP_006710507
          Length = 719

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   GILIKRQ 8
           GILIK+Q
Sbjct: 705 GILIKKQ 711
>NP_689910
          Length = 719

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   GILIKRQ 8
           GILIK+Q
Sbjct: 705 GILIKKQ 711
>NP_000284
          Length = 1093

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   GILIKRQ 8
           GIL+KR+
Sbjct: 745 GILLKRE 751
>NP_001027005
          Length = 1086

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   GILIKRQ 8
           GIL+KR+
Sbjct: 738 GILLKRE 744
>NP_001137432
          Length = 115

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  GILIKRQ 8
          GIL++RQ
Sbjct: 13 GILVQRQ 19
>NP_001275988
          Length = 327

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1  FGILIKRQ 8
          FG+ +K+Q
Sbjct: 79 FGLAVKKQ 86
>NP_001275990
          Length = 514

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   FGILIKRQ 8
           FG+ +K+Q
Sbjct: 266 FGLAVKKQ 273
>NP_001275987
          Length = 473

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   FGILIKRQ 8
           FG+ +K+Q
Sbjct: 225 FGLAVKKQ 232
>NP_112168
          Length = 514

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   FGILIKRQ 8
           FG+ +K+Q
Sbjct: 266 FGLAVKKQ 273
>NP_001137431
          Length = 194

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  GILIKRQ 8
          GIL++RQ
Sbjct: 92 GILVQRQ 98
>XP_005256041
          Length = 1086

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   GILIKRQ 8
           GIL+KR+
Sbjct: 738 GILLKRE 744
>XP_005256040
          Length = 1093

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   GILIKRQ 8
           GIL+KR+
Sbjct: 745 GILLKRE 751
>XP_006718343
          Length = 473

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   FGILIKRQ 8
           FG+ +K+Q
Sbjct: 225 FGLAVKKQ 232
>XP_006718342
          Length = 448

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   FGILIKRQ 8
           FG+ +K+Q
Sbjct: 266 FGLAVKKQ 273
>XP_006718341
          Length = 473

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   FGILIKRQ 8
           FG+ +K+Q
Sbjct: 225 FGLAVKKQ 232
>XP_005264347
          Length = 115

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  GILIKRQ 8
          GIL++RQ
Sbjct: 13 GILVQRQ 19
>XP_005264346
          Length = 115

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  GILIKRQ 8
          GIL++RQ
Sbjct: 13 GILVQRQ 19
>XP_005264345
          Length = 115

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  GILIKRQ 8
          GIL++RQ
Sbjct: 13 GILVQRQ 19
>XP_005264344
          Length = 194

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  GILIKRQ 8
          GIL++RQ
Sbjct: 92 GILVQRQ 98
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.341    0.158    0.438 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 475,426
Number of extensions: 699
Number of successful extensions: 40
Number of sequences better than 10000.0: 40
Number of HSP's gapped: 40
Number of HSP's successfully gapped: 40
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.4 bits)
S2: 28 (15.4 bits)

CANCERTOPE    |     Raghava's Group    |     IMTECH    |     CSIR    |     CRDD    |     GPSR Package