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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              20     390
NP_001075109                                                           20     390
NP_055394                                                              17    2528
NP_005500                                                              17    2528
NP_001277250                                                           17    2528
XP_006714623                                                           17    2528
XP_005271970                                                           17    2528
XP_005271969                                                           17    2528
XP_005271967                                                           17    2528
XP_005271966                                                           17    2528
NP_115991                                                              16    5631
NP_001025035                                                           16    7355
NP_114092                                                              16    7355
NP_064508                                                              15    9605
NP_808211                                                              15    9605
NP_803545                                                              15    9605
NP_258411                                                              15    9605
NP_005299                                                              15    9605
NP_003703                                                              15    9605
XP_005257845                                                           15    9605
XP_005257844                                                           15    9605
XP_005269765                                                           15    9605
XP_005269764                                                           15    9605
XP_005249598                                                           15    9605
XP_005249596                                                           15    9605
XP_005249594                                                           15    9605
NP_078991                                                              15    9605

>NP_008835
          Length = 4128

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    FFLESIAMY 9
            FFLESIAM+
Sbjct: 1296 FFLESIAMH 1304



>NP_001075109
          Length = 4097

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    FFLESIAMY 9
            FFLESIAM+
Sbjct: 1296 FFLESIAMH 1304



>NP_055394
          Length = 309

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   FFLESIAM 8
           FFLES+A+
Sbjct: 117 FFLESLAL 124



>NP_005500
          Length = 3027

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   FFLESIA 7
           FFLESIA
Sbjct: 107 FFLESIA 113



>NP_001277250
          Length = 3048

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   FFLESIA 7
           FFLESIA
Sbjct: 107 FFLESIA 113



>XP_006714623
          Length = 1625

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   FFLESIA 7
           FFLESIA
Sbjct: 107 FFLESIA 113



>XP_005271970
          Length = 2931

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   FFLESIA 7
           FFLESIA
Sbjct: 107 FFLESIA 113



>XP_005271969
          Length = 3020

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1  FFLESIA 7
          FFLESIA
Sbjct: 41 FFLESIA 47



>XP_005271967
          Length = 3065

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   FFLESIA 7
           FFLESIA
Sbjct: 107 FFLESIA 113



>XP_005271966
          Length = 3086

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   FFLESIA 7
           FFLESIA
Sbjct: 107 FFLESIA 113



>NP_115991
          Length = 543

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   FFLESIAMY 9
           FFL  + MY
Sbjct: 505 FFLPGVCMY 513



>NP_001025035
          Length = 1200

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FFLESIAMY 9
           FFL++ A Y
Sbjct: 888 FFLQAAAQY 896



>NP_114092
          Length = 456

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2  FLESIAMY 9
          FLE +A Y
Sbjct: 89 FLEEVAFY 96



>NP_064508
          Length = 589

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FFLESIAMY 9
           FF+  IA+Y
Sbjct: 375 FFINFIAIY 383



>NP_808211
          Length = 309

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FFLESIAMY 9
           FFL + A+Y
Sbjct: 222 FFLVAFALY 230



>NP_803545
          Length = 232

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FFLESIAMY 9
           FFL + A+Y
Sbjct: 145 FFLVAFALY 153



>NP_258411
          Length = 638

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   FFLESI 6
           FFLES+
Sbjct: 613 FFLESV 618



>NP_005299
          Length = 590

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  FLESIAMY 9
          FL+S+A Y
Sbjct: 83 FLDSVAEY 90



>NP_003703
          Length = 288

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FFLESIAMY 9
           FFL + A+Y
Sbjct: 201 FFLVAFALY 209



>XP_005257845
          Length = 400

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   FFLESI 6
           FFLES+
Sbjct: 375 FFLESV 380



>XP_005257844
          Length = 400

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   FFLESI 6
           FFLES+
Sbjct: 375 FFLESV 380



>XP_005269765
          Length = 558

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  FLESIAMY 9
          FL+S+A Y
Sbjct: 51 FLDSVAEY 58



>XP_005269764
          Length = 560

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  FLESIAMY 9
          FL+S+A Y
Sbjct: 83 FLDSVAEY 90



>XP_005249598
          Length = 1027

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  FFLESIAMY 9
          FFLES+  +
Sbjct: 10 FFLESLPAF 18



>XP_005249596
          Length = 1035

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  FFLESIAMY 9
          FFLES+  +
Sbjct: 10 FFLESLPAF 18



>XP_005249594
          Length = 1040

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  FFLESIAMY 9
          FFLES+  +
Sbjct: 10 FFLESLPAF 18



>NP_078991
          Length = 360

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   FFLESIAMY 9
           FFL S  MY
Sbjct: 331 FFLISFIMY 339



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.347    0.152    0.438 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 278,539
Number of extensions: 355
Number of successful extensions: 27
Number of sequences better than 10000.0: 27
Number of HSP's gapped: 27
Number of HSP's successfully gapped: 27
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 14 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.8 bits)
S2: 28 (15.4 bits)