Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_570711                                                              20     299
NP_570712                                                              20     299
NP_570714                                                              20     299
NP_570713                                                              20     299
NP_570716                                                              20     299
NP_000235                                                              20     299
NP_570715                                                              20     299
XP_006718620                                                           20     299
XP_005274059                                                           20     299
XP_005274060                                                           20     299
XP_005274058                                                           20     299
NP_001743                                                              17    2528
NP_057288                                                              17    3301
XP_006717824                                                           17    3301
NP_056483                                                              17    4312
NP_001273621                                                           17    4312
XP_005250917                                                           17    4312
XP_006716604                                                           17    4312
XP_005250916                                                           17    4312
XP_005250915                                                           17    4312
XP_005250914                                                           17    4312
XP_005250913                                                           17    4312
NP_065822                                                              16    5631
NP_006517                                                              16    5631
NP_001243779                                                           16    5631
NP_596869                                                              16    5631
NP_612420                                                              16    5631
XP_005260602                                                           16    5631
XP_006723938                                                           16    5631
XP_005258529                                                           16    5631
XP_006715593                                                           16    5631
XP_005267128                                                           16    5631
XP_006715592                                                           16    5631
XP_006715591                                                           16    5631
XP_006712787                                                           16    5631
XP_006712786                                                           16    5631
NP_001254479                                                           16    5631
NP_000825                                                              16    7355
NP_653314                                                              15    9605
NP_001035939                                                           15    9605
NP_776152                                                              15    9605
NP_001099003                                                           15    9605
NP_079189                                                              15    9605
NP_689690                                                              15    9605
NP_001001671                                                           15    9605
XP_005276312                                                           15    9605
XP_005258610                                                           15    9605
XP_005269575                                                           15    9605
XP_005250390                                                           15    9605
XP_005263448                                                           15    9605
XP_006712016                                                           15    9605
XP_005276022                                                           15    9605
NP_932351                                                              15    9605
>NP_570711
          Length = 610

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   FFEVANDV 8
           FFEVANDV
Sbjct: 364 FFEVANDV 371
>NP_570712
          Length = 615

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   FFEVANDV 8
           FFEVANDV
Sbjct: 369 FFEVANDV 376
>NP_570714
          Length = 615

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   FFEVANDV 8
           FFEVANDV
Sbjct: 369 FFEVANDV 376
>NP_570713
          Length = 615

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   FFEVANDV 8
           FFEVANDV
Sbjct: 369 FFEVANDV 376
>NP_570716
          Length = 615

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   FFEVANDV 8
           FFEVANDV
Sbjct: 369 FFEVANDV 376
>NP_000235
          Length = 615

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   FFEVANDV 8
           FFEVANDV
Sbjct: 369 FFEVANDV 376
>NP_570715
          Length = 615

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   FFEVANDV 8
           FFEVANDV
Sbjct: 369 FFEVANDV 376
>XP_006718620
          Length = 610

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   FFEVANDV 8
           FFEVANDV
Sbjct: 364 FFEVANDV 371
>XP_005274059
          Length = 610

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   FFEVANDV 8
           FFEVANDV
Sbjct: 364 FFEVANDV 371
>XP_005274060
          Length = 610

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   FFEVANDV 8
           FFEVANDV
Sbjct: 364 FFEVANDV 371
>XP_005274058
          Length = 610

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   FFEVANDV 8
           FFEVANDV
Sbjct: 364 FFEVANDV 371
>NP_001743
          Length = 527

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  FFEVANDVT 9
          +FEV +D+T
Sbjct: 84 YFEVTHDIT 92
>NP_057288
          Length = 429

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   FFEVANDVT 9
           FF +A+DVT
Sbjct: 361 FFPLADDVT 369
>XP_006717824
          Length = 417

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   FFEVANDVT 9
           FF +A+DVT
Sbjct: 361 FFPLADDVT 369
>NP_056483
          Length = 1264

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  FEVAND 7
          FEVAND
Sbjct: 54 FEVAND 59
>NP_001273621
          Length = 1252

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  FEVAND 7
          FEVAND
Sbjct: 54 FEVAND 59
>XP_005250917
          Length = 824

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  FEVAND 7
          FEVAND
Sbjct: 54 FEVAND 59
>XP_006716604
          Length = 1045

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  FEVAND 7
          FEVAND
Sbjct: 54 FEVAND 59
>XP_005250916
          Length = 1099

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  FEVAND 7
          FEVAND
Sbjct: 54 FEVAND 59
>XP_005250915
          Length = 1210

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  FEVAND 7
          FEVAND
Sbjct: 54 FEVAND 59
>XP_005250914
          Length = 1211

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  FEVAND 7
          FEVAND
Sbjct: 54 FEVAND 59
>XP_005250913
          Length = 1211

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  FEVAND 7
          FEVAND
Sbjct: 54 FEVAND 59
>NP_065822
          Length = 909

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FFEVANDVT 9
           F+EV  D+T
Sbjct: 816 FWEVVEDIT 824
>NP_006517
          Length = 1048

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   FFEVANDVT 9
           FF+ A DVT
Sbjct: 560 FFDGAKDVT 568
>NP_001243779
          Length = 34350

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2    FEVANDV 8
            FEV NDV
Sbjct: 5578 FEVQNDV 5584
>NP_596869
          Length = 33423

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2    FEVANDV 8
            FEV NDV
Sbjct: 4651 FEVQNDV 4657
>NP_612420
          Length = 231

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   EVANDVT 9
           EVAN+VT
Sbjct: 126 EVANEVT 132
>XP_005260602
          Length = 1061

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   FFEVANDVT 9
           FF+ A DVT
Sbjct: 560 FFDGAKDVT 568
>XP_006723938
          Length = 1061

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   FFEVANDVT 9
           FF+ A DVT
Sbjct: 560 FFDGAKDVT 568
>XP_005258529
          Length = 247

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   EVANDVT 9
           EVAN+VT
Sbjct: 142 EVANEVT 148
>XP_006715593
          Length = 741

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FFEVANDVT 9
           F+EV  D+T
Sbjct: 648 FWEVVEDIT 656
>XP_005267128
          Length = 741

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FFEVANDVT 9
           F+EV  D+T
Sbjct: 648 FWEVVEDIT 656
>XP_006715592
          Length = 865

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FFEVANDVT 9
           F+EV  D+T
Sbjct: 772 FWEVVEDIT 780
>XP_006715591
          Length = 875

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FFEVANDVT 9
           F+EV  D+T
Sbjct: 782 FWEVVEDIT 790
>XP_006712787
          Length = 33978

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2    FEVANDV 8
            FEV NDV
Sbjct: 5579 FEVQNDV 5585
>XP_006712786
          Length = 35484

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2    FEVANDV 8
            FEV NDV
Sbjct: 5579 FEVQNDV 5585
>NP_001254479
          Length = 35991

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2    FEVANDV 8
            FEV NDV
Sbjct: 5895 FEVQNDV 5901
>NP_000825
          Length = 1484

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   FEVANDV 8
           FEVAN V
Sbjct: 241 FEVANSV 247
>NP_653314
          Length = 573

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   FFEVANDVT 9
           FF +A+D++
Sbjct: 215 FFSLASDIS 223
>NP_001035939
          Length = 499

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  VANDVT 9
          +ANDVT
Sbjct: 67 IANDVT 72
>NP_776152
          Length = 1154

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   EVANDVT 9
           EVA DVT
Sbjct: 656 EVAKDVT 662
>NP_001099003
          Length = 783

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   FFEVANDV 8
           F++VAN V
Sbjct: 485 FYDVANPV 492
>NP_079189
          Length = 1026

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   FFEVANDV 8
           F++VAN V
Sbjct: 485 FYDVANPV 492
>NP_689690
          Length = 499

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  VANDVT 9
          +ANDVT
Sbjct: 67 IANDVT 72
>NP_001001671
          Length = 1313

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   FEVANDVT 9
           FE  N+VT
Sbjct: 517 FEATNEVT 524
>XP_005276312
          Length = 522

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FFEVANDVT 9
           F E+ N+VT
Sbjct: 482 FLELKNEVT 490
>XP_005258610
          Length = 499

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  VANDVT 9
          +ANDVT
Sbjct: 67 IANDVT 72
>XP_005269575
          Length = 1113

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   EVANDVT 9
           EVA DVT
Sbjct: 615 EVAKDVT 621
>XP_005250390
          Length = 231

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2  FEVANDVT 9
          F+V N++T
Sbjct: 46 FQVGNEIT 53
>XP_005263448
          Length = 522

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FFEVANDVT 9
           F E+ N+VT
Sbjct: 482 FLELKNEVT 490
>XP_006712016
          Length = 593

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   FFEVANDVT 9
           FF +A+D++
Sbjct: 215 FFSLASDIS 223
>XP_005276022
          Length = 522

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FFEVANDVT 9
           F E+ N+VT
Sbjct: 482 FLELKNEVT 490
>NP_932351
          Length = 305

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2  FEVANDVT 9
          F+V N++T
Sbjct: 46 FQVGNEIT 53
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.328    0.139    0.396 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 417,460
Number of extensions: 678
Number of successful extensions: 53
Number of sequences better than 10000.0: 53
Number of HSP's gapped: 53
Number of HSP's successfully gapped: 53
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)

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