Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001171795                                                           17    3301
NP_001171802                                                           17    3301
NP_001171796                                                           17    3301
NP_116127                                                              17    3301
XP_006711644                                                           17    3301
NP_001069                                                              16    5631
NP_001186763                                                           16    5631
NP_542413                                                              16    5631
NP_001274141                                                           16    5631
NP_003226                                                              16    5631
NP_001167554                                                           16    5631
NP_001167553                                                           16    5631
NP_060322                                                              16    5631
NP_065867                                                              16    5631
NP_001116291                                                           16    5631
NP_001167552                                                           16    5631
XP_005251100                                                           16    5631
XP_006716686                                                           16    5631
XP_005251099                                                           16    5631
XP_005251097                                                           16    5631
XP_005251095                                                           16    5631
XP_006716685                                                           16    5631
XP_005246008                                                           16    5631
XP_005246007                                                           16    5631
NP_061324                                                              16    5631
NP_001035943                                                           16    5631
NP_056099                                                              16    7355
NP_001138863                                                           16    7355
NP_060367                                                              16    7355
NP_001272333                                                           16    7355
NP_079351                                                              16    7355
NP_001272316                                                           16    7355
XP_006721553                                                           16    7355
XP_006721552                                                           16    7355
XP_006721551                                                           16    7355
XP_006721550                                                           16    7355
XP_005256620                                                           16    7355
XP_006713402                                                           16    7355
XP_006713279                                                           16    7355
XP_006710565                                                           16    7355
XP_006710564                                                           16    7355
XP_005270743                                                           16    7355
NP_000543                                                              15    9605
NP_006531                                                              15    9605
NP_001007098                                                           15    9605
NP_001018075                                                           15    9605
NP_006171                                                              15    9605
NP_001018074                                                           15    9605
NP_001018076                                                           15    9605
NP_001167558                                                           15    9605
NP_858045                                                              15    9605
NP_006525                                                              15    9605
NP_001167559                                                           15    9605
NP_060145                                                              15    9605
NP_001273206                                                           15    9605
NP_000151                                                              15    9605
NP_001165447                                                           15    9605
NP_001273207                                                           15    9605
NP_006313                                                              15    9605
NP_775922                                                              15    9605
NP_001274324                                                           15    9605
XP_006722340                                                           15    9605
XP_006722339                                                           15    9605
XP_005268350                                                           15    9605
XP_005252064                                                           15    9605
XP_005252063                                                           15    9605
XP_006717183                                                           15    9605
XP_005252061                                                           15    9605
XP_005252060                                                           15    9605
XP_005252058                                                           15    9605
XP_005251190                                                           15    9605
XP_005251188                                                           15    9605
XP_005251189                                                           15    9605
XP_005251187                                                           15    9605
XP_005251186                                                           15    9605
XP_005251185                                                           15    9605
XP_006714950                                                           15    9605
XP_006712547                                                           15    9605
>NP_001171795
          Length = 382

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  FETIQC 6
          FET+QC
Sbjct: 53 FETLQC 58
>NP_001171802
          Length = 192

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  FETIQC 6
          FET+QC
Sbjct: 47 FETLQC 52
>NP_001171796
          Length = 287

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  FETIQC 6
          FET+QC
Sbjct: 47 FETLQC 52
>NP_116127
          Length = 376

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  FETIQC 6
          FET+QC
Sbjct: 47 FETLQC 52
>XP_006711644
          Length = 293

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  FETIQC 6
          FET+QC
Sbjct: 53 FETLQC 58
>NP_001069
          Length = 739

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   TIQCSVA 9
           T++CSVA
Sbjct: 134 TVKCSVA 140
>NP_001186763
          Length = 677

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  TIQCSVA 9
          T++CSVA
Sbjct: 72 TVKCSVA 78
>NP_542413
          Length = 647

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   TIQCSVA 9
           T++CSVA
Sbjct: 134 TVKCSVA 140
>NP_001274141
          Length = 929

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1  FETIQCS 7
          FE++QC+
Sbjct: 22 FESVQCA 28
>NP_003226
          Length = 2768

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1  FETIQC 6
          F+T+QC
Sbjct: 58 FQTVQC 63
>NP_001167554
          Length = 1039

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   FETIQCS 7
           FET+ CS
Sbjct: 980 FETLTCS 986
>NP_001167553
          Length = 1040

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   FETIQCS 7
           FET+ CS
Sbjct: 981 FETLTCS 987
>NP_060322
          Length = 1062

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1    FETIQCS 7
            FET+ CS
Sbjct: 1003 FETLTCS 1009
>NP_065867
          Length = 1029

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   FETIQCS 7
           FE++QC+
Sbjct: 122 FESVQCA 128
>NP_001116291
          Length = 1028

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   FETIQCS 7
           FE++QC+
Sbjct: 122 FESVQCA 128
>NP_001167552
          Length = 1062

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1    FETIQCS 7
            FET+ CS
Sbjct: 1003 FETLTCS 1009
>XP_005251100
          Length = 2103

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1  FETIQC 6
          F+T+QC
Sbjct: 58 FQTVQC 63
>XP_006716686
          Length = 2111

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1  FETIQC 6
          F+T+QC
Sbjct: 58 FQTVQC 63
>XP_005251099
          Length = 2121

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1  FETIQC 6
          F+T+QC
Sbjct: 58 FQTVQC 63
>XP_005251097
          Length = 2178

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1  FETIQC 6
          F+T+QC
Sbjct: 58 FQTVQC 63
>XP_005251095
          Length = 2704

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1  FETIQC 6
          F+T+QC
Sbjct: 58 FQTVQC 63
>XP_006716685
          Length = 2747

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1  FETIQC 6
          F+T+QC
Sbjct: 58 FQTVQC 63
>XP_005246008
          Length = 984

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   FETIQCS 7
           FE++QC+
Sbjct: 122 FESVQCA 128
>XP_005246007
          Length = 985

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   FETIQCS 7
           FE++QC+
Sbjct: 122 FESVQCA 128
>NP_061324
          Length = 411

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   ETIQCS 7
           ET+QCS
Sbjct: 352 ETLQCS 357
>NP_001035943
          Length = 411

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   ETIQCS 7
           ET+QCS
Sbjct: 352 ETLQCS 357
>NP_056099
          Length = 3375

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   FETIQCSVA 9
           + TIQC ++
Sbjct: 605 YSTIQCRIS 613
>NP_001138863
          Length = 376

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  FETIQCSVA 9
          +++I CSVA
Sbjct: 81 YKSIPCSVA 89
>NP_060367
          Length = 459

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  FETIQCSVA 9
          +++I CSVA
Sbjct: 81 YKSIPCSVA 89
>NP_001272333
          Length = 152

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1  FETIQCSVA 9
          ++ I+CS+A
Sbjct: 33 YKEIECSIA 41
>NP_079351
          Length = 204

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1  FETIQCSVA 9
          ++ I+CS+A
Sbjct: 85 YKEIECSIA 93
>NP_001272316
          Length = 152

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1  FETIQCSVA 9
          ++ I+CS+A
Sbjct: 33 YKEIECSIA 41
>XP_006721553
          Length = 2286

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1    FETIQC 6
            F TIQC
Sbjct: 1238 FSTIQC 1243
>XP_006721552
          Length = 2299

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1    FETIQC 6
            F TIQC
Sbjct: 1238 FSTIQC 1243
>XP_006721551
          Length = 2301

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1    FETIQC 6
            F TIQC
Sbjct: 1200 FSTIQC 1205
>XP_006721550
          Length = 2326

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1    FETIQC 6
            F TIQC
Sbjct: 1238 FSTIQC 1243
>XP_005256620
          Length = 2339

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1    FETIQC 6
            F TIQC
Sbjct: 1238 FSTIQC 1243
>XP_006713402
          Length = 152

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1  FETIQCSVA 9
          ++ I+CS+A
Sbjct: 33 YKEIECSIA 41
>XP_006713279
          Length = 459

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  FETIQCSVA 9
          +++I CSVA
Sbjct: 81 YKSIPCSVA 89
>XP_006710565
          Length = 3140

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   FETIQCSVA 9
           + TIQC ++
Sbjct: 313 YSTIQCRIS 321
>XP_006710564
          Length = 3432

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   FETIQCSVA 9
           + TIQC ++
Sbjct: 605 YSTIQCRIS 613
>XP_005270743
          Length = 3453

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   FETIQCSVA 9
           + TIQC ++
Sbjct: 626 YSTIQCRIS 634
>NP_000543
          Length = 2813

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 2   ETIQCS 7
           ET+QC+
Sbjct: 428 ETVQCA 433
>NP_006531
          Length = 1464

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1   FETIQC 6
           +ET+QC
Sbjct: 224 YETMQC 229
>NP_001007098
          Length = 477

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   TIQCSVA 9
           T+ CSVA
Sbjct: 215 TLSCSVA 221
>NP_001018075
          Length = 553

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   TIQCSVA 9
           T+ CSVA
Sbjct: 215 TLSCSVA 221
>NP_006171
          Length = 838

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   TIQCSVA 9
           T+ CSVA
Sbjct: 215 TLSCSVA 221
>NP_001018074
          Length = 822

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   TIQCSVA 9
           T+ CSVA
Sbjct: 215 TLSCSVA 221
>NP_001018076
          Length = 537

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   TIQCSVA 9
           T+ CSVA
Sbjct: 215 TLSCSVA 221
>NP_001167558
          Length = 1423

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1   FETIQC 6
           +ET+QC
Sbjct: 221 YETMQC 226
>NP_858045
          Length = 1424

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1   FETIQC 6
           +ET+QC
Sbjct: 221 YETMQC 226
>NP_006525
          Length = 1420

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1   FETIQC 6
           +ET+QC
Sbjct: 221 YETMQC 226
>NP_001167559
          Length = 1415

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1   FETIQC 6
           +ET+QC
Sbjct: 221 YETMQC 226
>NP_060145
          Length = 1310

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   TIQCSV 8
           TIQCS+
Sbjct: 218 TIQCSL 223
>NP_001273206
          Length = 897

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1   FETIQCS 7
           FE ++CS
Sbjct: 714 FEVLECS 720
>NP_000151
          Length = 477

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 5/6 (83%)

Query: 1   FETIQC 6
           FE +QC
Sbjct: 289 FENVQC 294
>NP_001165447
          Length = 1310

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   TIQCSV 8
           TIQCS+
Sbjct: 218 TIQCSL 223
>NP_001273207
          Length = 824

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1   FETIQCS 7
           FE ++CS
Sbjct: 724 FEVLECS 730
>NP_006313
          Length = 907

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1   FETIQCS 7
           FE ++CS
Sbjct: 724 FEVLECS 730
>NP_775922
          Length = 6996

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3    TIQCSVA 9
            T++CS+A
Sbjct: 2206 TLKCSIA 2212
>NP_001274324
          Length = 1688

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 1    FETIQCSV 8
            F+T  CS+
Sbjct: 1584 FQTTMCSI 1591
>XP_006722340
          Length = 477

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 5/6 (83%)

Query: 1   FETIQC 6
           FE +QC
Sbjct: 289 FENVQC 294
>XP_006722339
          Length = 523

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 5/6 (83%)

Query: 1   FETIQC 6
           FE +QC
Sbjct: 319 FENVQC 324
>XP_005268350
          Length = 818

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1   FETIQCS 7
           FE ++CS
Sbjct: 724 FEVLECS 730
>XP_005252064
          Length = 477

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   TIQCSVA 9
           T+ CSVA
Sbjct: 215 TLSCSVA 221
>XP_005252063
          Length = 553

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   TIQCSVA 9
           T+ CSVA
Sbjct: 215 TLSCSVA 221
>XP_006717183
          Length = 554

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   TIQCSVA 9
           T+ CSVA
Sbjct: 215 TLSCSVA 221
>XP_005252061
          Length = 838

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   TIQCSVA 9
           T+ CSVA
Sbjct: 215 TLSCSVA 221
>XP_005252060
          Length = 838

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   TIQCSVA 9
           T+ CSVA
Sbjct: 215 TLSCSVA 221
>XP_005252058
          Length = 838

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   TIQCSVA 9
           T+ CSVA
Sbjct: 215 TLSCSVA 221
>XP_005251190
          Length = 1395

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1   FETIQC 6
           +ET+QC
Sbjct: 224 YETMQC 229
>XP_005251188
          Length = 1464

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1   FETIQC 6
           +ET+QC
Sbjct: 224 YETMQC 229
>XP_005251189
          Length = 1464

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1   FETIQC 6
           +ET+QC
Sbjct: 224 YETMQC 229
>XP_005251187
          Length = 1464

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1   FETIQC 6
           +ET+QC
Sbjct: 224 YETMQC 229
>XP_005251186
          Length = 1464

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1   FETIQC 6
           +ET+QC
Sbjct: 224 YETMQC 229
>XP_005251185
          Length = 1464

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1   FETIQC 6
           +ET+QC
Sbjct: 224 YETMQC 229
>XP_006714950
          Length = 1304

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   TIQCSV 8
           TIQCS+
Sbjct: 218 TIQCSL 223
>XP_006712547
          Length = 1674

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 1    FETIQCSV 8
            F+T  CS+
Sbjct: 1570 FQTTMCSI 1577
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.329    0.129    0.390 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 653,801
Number of extensions: 887
Number of successful extensions: 78
Number of sequences better than 10000.0: 78
Number of HSP's gapped: 78
Number of HSP's successfully gapped: 78
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)

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