Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              20     299
NP_001075109                                                           20     299
NP_937791                                                              17    3301
XP_005269585                                                           17    3301
XP_005260020                                                           17    4312
NP_060152                                                              17    4312
NP_006505                                                              16    5631
XP_005265428                                                           16    5631
NP_000264                                                              15    9605
NP_008995                                                              15    9605
NP_001019831                                                           15    9605
NP_009049                                                              15    9605
NP_060599                                                              15    9605
NP_699197                                                              15    9605
XP_005274599                                                           15    9605
XP_005274598                                                           15    9605
XP_005257557                                                           15    9605
XP_006714558                                                           15    9605
XP_006713877                                                           15    9605
XP_006713876                                                           15    9605
XP_006713875                                                           15    9605
XP_006713874                                                           15    9605
XP_006713873                                                           15    9605
>NP_008835
          Length = 4128

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    FESLVIYME 9
            FE+LVIYME
Sbjct: 1101 FEALVIYME 1109
>NP_001075109
          Length = 4097

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    FESLVIYME 9
            FE+LVIYME
Sbjct: 1101 FEALVIYME 1109
>NP_937791
          Length = 756

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   ESLVIYME 9
           ESL+++ME
Sbjct: 631 ESLIVFME 638
>XP_005269585
          Length = 704

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   ESLVIYME 9
           ESL+++ME
Sbjct: 631 ESLIVFME 638
>XP_005260020
          Length = 509

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  FESLVIYME 9
          FE LVIY +
Sbjct: 62 FEKLVIYCD 70
>NP_060152
          Length = 509

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  FESLVIYME 9
          FE LVIY +
Sbjct: 62 FEKLVIYCD 70
>NP_006505
          Length = 1956

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    FESLVIYM 8
            FES +I+M
Sbjct: 1154 FESFIIFM 1161
>XP_005265428
          Length = 1959

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    FESLVIYM 8
            FES +I+M
Sbjct: 1157 FESFIIFM 1164
>NP_000264
          Length = 404

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   ESLVIYME 9
           ESL+ Y+E
Sbjct: 264 ESLLFYLE 271
>NP_008995
          Length = 1289

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FESLVIYME 9
           FE +VI+ E
Sbjct: 453 FEQIVIFSE 461
>NP_001019831
          Length = 2986

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    FESLVIYME 9
            FE +VI+ E
Sbjct: 2150 FEQIVIFSE 2158
>NP_009049
          Length = 3097

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    FESLVIYME 9
            FE +VI+ E
Sbjct: 2191 FEQIVIFSE 2199
>NP_060599
          Length = 261

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   FESLVIYME 9
           F SLV Y E
Sbjct: 126 FASLVFYWE 134
>NP_699197
          Length = 3571

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1    FESLVIY 7
            FES +IY
Sbjct: 3259 FESTIIY 3265
>XP_005274599
          Length = 380

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   ESLVIYME 9
           ESL+ Y+E
Sbjct: 264 ESLLFYLE 271
>XP_005274598
          Length = 386

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   ESLVIYME 9
           ESL+ Y+E
Sbjct: 264 ESLLFYLE 271
>XP_005257557
          Length = 181

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  FESLVIYME 9
          F SLV Y E
Sbjct: 46 FASLVFYWE 54
>XP_006714558
          Length = 2612

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    FESLVIYME 9
            FE +VI+ E
Sbjct: 1706 FEQIVIFSE 1714
>XP_006713877
          Length = 2956

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    FESLVIYME 9
            FE +VI+ E
Sbjct: 2120 FEQIVIFSE 2128
>XP_006713876
          Length = 2966

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    FESLVIYME 9
            FE +VI+ E
Sbjct: 2130 FEQIVIFSE 2138
>XP_006713875
          Length = 2975

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    FESLVIYME 9
            FE +VI+ E
Sbjct: 2139 FEQIVIFSE 2147
>XP_006713874
          Length = 2979

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    FESLVIYME 9
            FE +VI+ E
Sbjct: 2143 FEQIVIFSE 2151
>XP_006713873
          Length = 2988

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    FESLVIYME 9
            FE +VI+ E
Sbjct: 2152 FEQIVIFSE 2160
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.331    0.145    0.382 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 374,891
Number of extensions: 623
Number of successful extensions: 23
Number of sequences better than 10000.0: 23
Number of HSP's gapped: 23
Number of HSP's successfully gapped: 23
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)

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