Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_733751                                                              19     665
XP_006716142                                                           19     665
XP_005250088                                                           19     665
XP_006716141                                                           19     665
XP_005250085                                                           19     665
XP_006716140                                                           19     665
XP_005250084                                                           19     665
XP_005250083                                                           19     665
XP_005250082                                                           19     665
NP_002963                                                              17    3301
NP_006575                                                              17    3301
NP_001180459                                                           17    3301
NP_001180458                                                           17    3301
XP_005261992                                                           17    3301
XP_005261991                                                           17    3301
XP_005261989                                                           17    3301
XP_005261988                                                           17    3301
XP_006721705                                                           17    3301
XP_006721704                                                           17    3301
NP_075054                                                              17    3301
NP_006297                                                              16    7355
NP_001268392                                                           16    7355
NP_001177652                                                           15    9605
NP_001005374                                                           15    9605
NP_612370                                                              15    9605
NP_001005373                                                           15    9605
NP_073597                                                              15    9605
NP_001258097                                                           15    9605
XP_006723672                                                           15    9605
XP_005260863                                                           15    9605
XP_005260862                                                           15    9605
XP_005260861                                                           15    9605
XP_006717379                                                           15    9605
>NP_733751
          Length = 4911

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    FEAAQVKAK 9
            FEAAQV+AK
Sbjct: 4313 FEAAQVEAK 4321
>XP_006716142
          Length = 4927

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    FEAAQVKAK 9
            FEAAQV+AK
Sbjct: 4329 FEAAQVEAK 4337
>XP_005250088
          Length = 4928

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    FEAAQVKAK 9
            FEAAQV+AK
Sbjct: 4330 FEAAQVEAK 4338
>XP_006716141
          Length = 4959

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    FEAAQVKAK 9
            FEAAQV+AK
Sbjct: 4361 FEAAQVEAK 4369
>XP_005250085
          Length = 4979

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    FEAAQVKAK 9
            FEAAQV+AK
Sbjct: 4385 FEAAQVEAK 4393
>XP_006716140
          Length = 4982

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    FEAAQVKAK 9
            FEAAQV+AK
Sbjct: 4384 FEAAQVEAK 4392
>XP_005250084
          Length = 4982

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    FEAAQVKAK 9
            FEAAQV+AK
Sbjct: 4384 FEAAQVEAK 4392
>XP_005250083
          Length = 4982

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    FEAAQVKAK 9
            FEAAQV+AK
Sbjct: 4384 FEAAQVEAK 4392
>XP_005250082
          Length = 4983

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    FEAAQVKAK 9
            FEAAQV+AK
Sbjct: 4385 FEAAQVEAK 4393
>NP_002963
          Length = 1893

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1    FEAAQVKA 8
            FEA QVKA
Sbjct: 1380 FEARQVKA 1387
>NP_006575
          Length = 530

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   FEAAQVKA 8
           FEAA++KA
Sbjct: 123 FEAAKIKA 130
>NP_001180459
          Length = 485

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  FEAAQVKA 8
          FEAA++KA
Sbjct: 78 FEAAKIKA 85
>NP_001180458
          Length = 493

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   FEAAQVKA 8
           FEAA++KA
Sbjct: 123 FEAAKIKA 130
>XP_005261992
          Length = 1867

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1    FEAAQVKA 8
            FEA QVKA
Sbjct: 1354 FEARQVKA 1361
>XP_005261991
          Length = 1868

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1    FEAAQVKA 8
            FEA QVKA
Sbjct: 1355 FEARQVKA 1362
>XP_005261989
          Length = 1891

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1    FEAAQVKA 8
            FEA QVKA
Sbjct: 1378 FEARQVKA 1385
>XP_005261988
          Length = 1894

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1    FEAAQVKA 8
            FEA QVKA
Sbjct: 1381 FEARQVKA 1388
>XP_006721705
          Length = 464

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  FEAAQVKA 8
          FEAA++KA
Sbjct: 57 FEAAKIKA 64
>XP_006721704
          Length = 516

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   FEAAQVKA 8
           FEAA++KA
Sbjct: 123 FEAAKIKA 130
>NP_075054
          Length = 467

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   EAAQVKAK 9
           +AAQVKAK
Sbjct: 314 KAAQVKAK 321

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   EAAQVKAK 9
           +AAQVKAK
Sbjct: 325 KAAQVKAK 332
>NP_006297
          Length = 1233

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    FEAAQVKAK 9
            FEAA+ +AK
Sbjct: 1045 FEAARKRAK 1053
>NP_001268392
          Length = 1211

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    FEAAQVKAK 9
            FEAA+ +AK
Sbjct: 1023 FEAARKRAK 1031
>NP_001177652
          Length = 696

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   FEAAQVK 7
           FEA QVK
Sbjct: 457 FEALQVK 463
>NP_001005374
          Length = 723

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   FEAAQVK 7
           FEA QVK
Sbjct: 457 FEALQVK 463
>NP_612370
          Length = 723

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   FEAAQVK 7
           FEA QVK
Sbjct: 457 FEALQVK 463
>NP_001005373
          Length = 723

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   FEAAQVK 7
           FEA QVK
Sbjct: 457 FEALQVK 463
>NP_073597
          Length = 454

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  AAQVKAK 9
          AAQ+KAK
Sbjct: 62 AAQLKAK 68
>NP_001258097
          Length = 403

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  AAQVKAK 9
          AAQ+KAK
Sbjct: 62 AAQLKAK 68
>XP_006723672
          Length = 203

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  AAQVKAK 9
          AAQ+KAK
Sbjct: 62 AAQLKAK 68
>XP_005260863
          Length = 362

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  AAQVKAK 9
          AAQ+KAK
Sbjct: 62 AAQLKAK 68
>XP_005260862
          Length = 403

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  AAQVKAK 9
          AAQ+KAK
Sbjct: 62 AAQLKAK 68
>XP_005260861
          Length = 454

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  AAQVKAK 9
          AAQ+KAK
Sbjct: 62 AAQLKAK 68
>XP_006717379
          Length = 690

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   FEAAQVK 7
           FEA QVK
Sbjct: 457 FEALQVK 463
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.312    0.116    0.279 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 215,017
Number of extensions: 232
Number of successful extensions: 34
Number of sequences better than 10000.0: 33
Number of HSP's gapped: 34
Number of HSP's successfully gapped: 34
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)

CANCERTOPE    |     Raghava's Group    |     IMTECH    |     CSIR    |     CRDD    |     GPSR Package