Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001257436                                                           19     665
NP_001257437                                                           19     665
NP_006281                                                              19     665
XP_006715618                                                           19     665
XP_005267176                                                           19     665
NP_056161                                                              17    3301
XP_006720156                                                           17    3301
NP_443076                                                              16    5631
NP_690051                                                              16    5631
NP_006038                                                              16    5631
NP_001185769                                                           16    5631
NP_001185767                                                           16    5631
NP_068772                                                              15    9605
NP_001230018                                                           15    9605
NP_973732                                                              15    9605
NP_973731                                                              15    9605
NP_001230017                                                           15    9605
NP_000279                                                              15    9605
NP_115495                                                              15    9605
NP_005384                                                              15    9605
NP_054825                                                              15    9605
NP_001156729                                                           15    9605
XP_005253733                                                           15    9605
XP_005267076                                                           15    9605
>NP_001257436
          Length = 790

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   FASASGKVS 9
           FA+ASGKVS
Sbjct: 637 FAAASGKVS 645
>NP_001257437
          Length = 790

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   FASASGKVS 9
           FA+ASGKVS
Sbjct: 637 FAAASGKVS 645
>NP_006281
          Length = 790

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   FASASGKVS 9
           FA+ASGKVS
Sbjct: 637 FAAASGKVS 645
>XP_006715618
          Length = 577

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   FASASGKVS 9
           FA+ASGKVS
Sbjct: 424 FAAASGKVS 432
>XP_005267176
          Length = 790

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   FASASGKVS 9
           FA+ASGKVS
Sbjct: 637 FAAASGKVS 645
>NP_056161
          Length = 2539

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   ASASGKVS 9
           A+ASGKVS
Sbjct: 290 ATASGKVS 297
>XP_006720156
          Length = 2497

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   ASASGKVS 9
           A+ASGKVS
Sbjct: 290 ATASGKVS 297
>NP_443076
          Length = 536

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   FASASGK 7
           FA+ASGK
Sbjct: 451 FAAASGK 457
>NP_690051
          Length = 932

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   FASASGK 7
           FAS+SGK
Sbjct: 846 FASSSGK 852
>NP_006038
          Length = 932

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   FASASGK 7
           FAS+SGK
Sbjct: 846 FASSSGK 852
>NP_001185769
          Length = 932

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   FASASGK 7
           FAS+SGK
Sbjct: 846 FASSSGK 852
>NP_001185767
          Length = 932

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   FASASGK 7
           FAS+SGK
Sbjct: 846 FASSSGK 852
>NP_068772
          Length = 763

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SASGKVS 9
           SA+GKVS
Sbjct: 300 SANGKVS 306
>NP_001230018
          Length = 747

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SASGKVS 9
           SA+GKVS
Sbjct: 299 SANGKVS 305
>NP_973732
          Length = 748

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SASGKVS 9
           SA+GKVS
Sbjct: 300 SANGKVS 306
>NP_973731
          Length = 801

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SASGKVS 9
           SA+GKVS
Sbjct: 300 SANGKVS 306
>NP_001230017
          Length = 748

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SASGKVS 9
           SA+GKVS
Sbjct: 299 SANGKVS 305
>NP_000279
          Length = 323

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   FASASG 6
           FASASG
Sbjct: 171 FASASG 176
>NP_115495
          Length = 6306

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    FASASGKVS 9
            FA+ASG ++
Sbjct: 1627 FANASGTIT 1635
>NP_005384
          Length = 1909

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    FASASG 6
            FASASG
Sbjct: 1133 FASASG 1138
>NP_054825
          Length = 691

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   FASASGKV 8
           FA+ SGK+
Sbjct: 315 FAARSGKI 322
>NP_001156729
          Length = 1932

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    FASASG 6
            FASASG
Sbjct: 1156 FASASG 1161
>XP_005253733
          Length = 764

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SASGKVS 9
           SA+GKVS
Sbjct: 300 SANGKVS 306
>XP_005267076
          Length = 285

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   FASASG 6
           FASASG
Sbjct: 133 FASASG 138
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.303    0.110    0.267 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 446,165
Number of extensions: 499
Number of successful extensions: 24
Number of sequences better than 10000.0: 24
Number of HSP's gapped: 24
Number of HSP's successfully gapped: 24
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.4 bits)
S2: 28 (15.4 bits)

CANCERTOPE    |     Raghava's Group    |     IMTECH    |     CSIR    |     CRDD    |     GPSR Package