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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000360                                                              20     390
XP_005268094                                                           20     390
NP_001131074                                                           16    5631
NP_057039                                                              16    5631
XP_005264409                                                           16    5631
XP_005264408                                                           16    5631
NP_001193770                                                           16    7355
NP_001990                                                              15    9605

>NP_000360
          Length = 764

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   FAMRLDQKI 9
           FAMRLD+KI
Sbjct: 525 FAMRLDRKI 533



>XP_005268094
          Length = 764

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   FAMRLDQKI 9
           FAMRLD+KI
Sbjct: 525 FAMRLDRKI 533



>NP_001131074
          Length = 274

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  FAMRLDQKI 9
          + +R+DQKI
Sbjct: 78 YDLRIDQKI 86



>NP_057039
          Length = 297

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   FAMRLDQKI 9
           + +R+DQKI
Sbjct: 101 YDLRIDQKI 109



>XP_005264409
          Length = 170

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   FAMRLDQKI 9
           + +R+DQKI
Sbjct: 101 YDLRIDQKI 109



>XP_005264408
          Length = 297

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   FAMRLDQKI 9
           + +R+DQKI
Sbjct: 101 YDLRIDQKI 109



>NP_001193770
          Length = 453

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   RLDQKI 9
           RLDQKI
Sbjct: 432 RLDQKI 437



>NP_001990
          Length = 2912

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    FAMRLDQKI 9
            +A+R DQK+
Sbjct: 2283 YALREDQKM 2291



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.343    0.148    0.402 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 402,708
Number of extensions: 400
Number of successful extensions: 8
Number of sequences better than 10000.0: 8
Number of HSP's gapped: 8
Number of HSP's successfully gapped: 8
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.6 bits)
S2: 28 (15.4 bits)