Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000258                                                              19     869
NP_001035957                                                           19     869
XP_006721990                                                           19     869
XP_006721989                                                           19     869
XP_005258041                                                           19     869
XP_006721988                                                           19     869
XP_006721987                                                           19     869
XP_006721986                                                           19     869
XP_005258040                                                           19     869
XP_006721985                                                           19     869
XP_005247366                                                           16    5631
XP_006713639                                                           16    5631
XP_005247365                                                           16    5631
XP_005247364                                                           16    5631
XP_005247363                                                           16    5631
XP_006713637                                                           16    5631
XP_006713636                                                           16    5631
XP_005247362                                                           16    5631
XP_005247360                                                           16    5631
XP_006713635                                                           16    5631
XP_006713634                                                           16    5631
XP_005247359                                                           16    5631
XP_005247358                                                           16    5631
XP_006713633                                                           16    5631
XP_005247357                                                           16    5631
XP_006713632                                                           16    5631
XP_005247356                                                           16    5631
XP_005247355                                                           16    5631
XP_005247354                                                           16    5631
XP_005247353                                                           16    5631
XP_005247352                                                           16    5631
XP_005247351                                                           16    5631
XP_005247350                                                           16    5631
XP_005247348                                                           16    5631
XP_005247347                                                           16    5631
XP_005247346                                                           16    5631
XP_005247345                                                           16    5631
XP_005247344                                                           16    5631
XP_005247343                                                           16    5631
XP_005247342                                                           16    5631
XP_005247341                                                           16    5631
XP_005247340                                                           16    5631
XP_005247339                                                           16    5631
XP_006713631                                                           16    5631
XP_005247338                                                           16    5631
NP_114091                                                              16    7355
NP_001274152                                                           16    7355
NP_001269285                                                           16    7355
NP_054858                                                              16    7355
NP_599028                                                              16    7355
NP_439897                                                              16    7355
XP_005256139                                                           16    7355
XP_005256138                                                           16    7355
XP_005256137                                                           16    7355
NP_001073883                                                           15    9605
NP_775901                                                              15    9605
XP_005266736                                                           15    9605
XP_006722498                                                           15    9605
XP_006722497                                                           15    9605
XP_005267748                                                           15    9605
>NP_000258
          Length = 2818

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    FALTSLET 8
            FALTSLET
Sbjct: 2176 FALTSLET 2183
>NP_001035957
          Length = 2839

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    FALTSLET 8
            FALTSLET
Sbjct: 2197 FALTSLET 2204
>XP_006721990
          Length = 2823

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    FALTSLET 8
            FALTSLET
Sbjct: 2207 FALTSLET 2214
>XP_006721989
          Length = 2823

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    FALTSLET 8
            FALTSLET
Sbjct: 2207 FALTSLET 2214
>XP_005258041
          Length = 2836

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    FALTSLET 8
            FALTSLET
Sbjct: 2176 FALTSLET 2183
>XP_006721988
          Length = 2846

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    FALTSLET 8
            FALTSLET
Sbjct: 2186 FALTSLET 2193
>XP_006721987
          Length = 2849

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    FALTSLET 8
            FALTSLET
Sbjct: 2207 FALTSLET 2214
>XP_006721986
          Length = 2854

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    FALTSLET 8
            FALTSLET
Sbjct: 2194 FALTSLET 2201
>XP_005258040
          Length = 2857

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    FALTSLET 8
            FALTSLET
Sbjct: 2197 FALTSLET 2204
>XP_006721985
          Length = 2867

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    FALTSLET 8
            FALTSLET
Sbjct: 2207 FALTSLET 2214
>XP_005247366
          Length = 1495

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FALTSLET 8
           FALT L+T
Sbjct: 805 FALTELQT 812
>XP_006713639
          Length = 1579

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FALTSLET 8
           FALT L+T
Sbjct: 889 FALTELQT 896
>XP_005247365
          Length = 1607

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FALTSLET 8
           FALT L+T
Sbjct: 917 FALTELQT 924
>XP_005247364
          Length = 1637

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FALTSLET 8
           FALT L+T
Sbjct: 947 FALTELQT 954
>XP_005247363
          Length = 1657

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FALTSLET 8
           FALT L+T
Sbjct: 967 FALTELQT 974
>XP_006713637
          Length = 1659

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FALTSLET 8
           FALT L+T
Sbjct: 969 FALTELQT 976
>XP_006713636
          Length = 1673

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FALTSLET 8
           FALT L+T
Sbjct: 983 FALTELQT 990
>XP_005247362
          Length = 1678

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FALTSLET 8
           FALT L+T
Sbjct: 988 FALTELQT 995
>XP_005247360
          Length = 1706

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    FALTSLET 8
            FALT L+T
Sbjct: 1016 FALTELQT 1023
>XP_006713635
          Length = 1719

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    FALTSLET 8
            FALT L+T
Sbjct: 1029 FALTELQT 1036
>XP_006713634
          Length = 1721

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    FALTSLET 8
            FALT L+T
Sbjct: 1031 FALTELQT 1038
>XP_005247359
          Length = 1725

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    FALTSLET 8
            FALT L+T
Sbjct: 1035 FALTELQT 1042
>XP_005247358
          Length = 1726

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    FALTSLET 8
            FALT L+T
Sbjct: 1103 FALTELQT 1110
>XP_006713633
          Length = 1731

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    FALTSLET 8
            FALT L+T
Sbjct: 1041 FALTELQT 1048
>XP_005247357
          Length = 1735

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    FALTSLET 8
            FALT L+T
Sbjct: 1045 FALTELQT 1052
>XP_006713632
          Length = 1742

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    FALTSLET 8
            FALT L+T
Sbjct: 1103 FALTELQT 1110
>XP_005247356
          Length = 1744

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    FALTSLET 8
            FALT L+T
Sbjct: 1054 FALTELQT 1061
>XP_005247355
          Length = 1745

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    FALTSLET 8
            FALT L+T
Sbjct: 1055 FALTELQT 1062
>XP_005247354
          Length = 1745

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    FALTSLET 8
            FALT L+T
Sbjct: 1055 FALTELQT 1062
>XP_005247353
          Length = 1746

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    FALTSLET 8
            FALT L+T
Sbjct: 1056 FALTELQT 1063
>XP_005247352
          Length = 1747

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    FALTSLET 8
            FALT L+T
Sbjct: 1057 FALTELQT 1064
>XP_005247351
          Length = 1754

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    FALTSLET 8
            FALT L+T
Sbjct: 1064 FALTELQT 1071
>XP_005247350
          Length = 1757

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    FALTSLET 8
            FALT L+T
Sbjct: 1103 FALTELQT 1110
>XP_005247348
          Length = 1767

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    FALTSLET 8
            FALT L+T
Sbjct: 1103 FALTELQT 1110
>XP_005247347
          Length = 1768

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    FALTSLET 8
            FALT L+T
Sbjct: 1103 FALTELQT 1110
>XP_005247346
          Length = 1768

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    FALTSLET 8
            FALT L+T
Sbjct: 1078 FALTELQT 1085
>XP_005247345
          Length = 1769

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    FALTSLET 8
            FALT L+T
Sbjct: 1079 FALTELQT 1086
>XP_005247344
          Length = 1771

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    FALTSLET 8
            FALT L+T
Sbjct: 1103 FALTELQT 1110
>XP_005247343
          Length = 1771

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    FALTSLET 8
            FALT L+T
Sbjct: 1081 FALTELQT 1088
>XP_005247342
          Length = 1773

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    FALTSLET 8
            FALT L+T
Sbjct: 1083 FALTELQT 1090
>XP_005247341
          Length = 1774

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    FALTSLET 8
            FALT L+T
Sbjct: 1084 FALTELQT 1091
>XP_005247340
          Length = 1777

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    FALTSLET 8
            FALT L+T
Sbjct: 1103 FALTELQT 1110
>XP_005247339
          Length = 1779

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    FALTSLET 8
            FALT L+T
Sbjct: 1089 FALTELQT 1096
>XP_006713631
          Length = 1789

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    FALTSLET 8
            FALT L+T
Sbjct: 1099 FALTELQT 1106
>XP_005247338
          Length = 1793

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    FALTSLET 8
            FALT L+T
Sbjct: 1103 FALTELQT 1110
>NP_114091
          Length = 721

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   FALTSLETA 9
           +ALTSL+ A
Sbjct: 484 YALTSLDPA 492
>NP_001274152
          Length = 1791

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   ALTSLETA 9
           +LTSLET+
Sbjct: 444 SLTSLETS 451
>NP_001269285
          Length = 656

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   FALTSLETA 9
           FALTSL +A
Sbjct: 104 FALTSLISA 112
>NP_054858
          Length = 1791

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   ALTSLETA 9
           +LTSLET+
Sbjct: 444 SLTSLETS 451
>NP_599028
          Length = 663

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   FALTSLETA 9
           FALTSL +A
Sbjct: 104 FALTSLISA 112
>NP_439897
          Length = 656

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   FALTSLETA 9
           FALTSL +A
Sbjct: 104 FALTSLISA 112
>XP_005256139
          Length = 509

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   FALTSLETA 9
           +ALTSL+ A
Sbjct: 484 YALTSLDPA 492
>XP_005256138
          Length = 510

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   FALTSLETA 9
           +ALTSL+ A
Sbjct: 484 YALTSLDPA 492
>XP_005256137
          Length = 721

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   FALTSLETA 9
           +ALTSL+ A
Sbjct: 484 YALTSLDPA 492
>NP_001073883
          Length = 2028

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LTSLETA 9
           +TSLETA
Sbjct: 651 VTSLETA 657
>NP_775901
          Length = 2226

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    ALTSLET 8
            ALT+LET
Sbjct: 1392 ALTTLET 1398
>XP_005266736
          Length = 1314

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   ALTSLET 8
           ALT+LET
Sbjct: 480 ALTTLET 486
>XP_006722498
          Length = 2059

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    ALTSLET 8
            ALT+LET
Sbjct: 1393 ALTTLET 1399
>XP_006722497
          Length = 2227

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    ALTSLET 8
            ALT+LET
Sbjct: 1393 ALTTLET 1399
>XP_005267748
          Length = 1535

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LTSLETA 9
           +TSLETA
Sbjct: 651 VTSLETA 657
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.316    0.120    0.289 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 272,865
Number of extensions: 404
Number of successful extensions: 60
Number of sequences better than 10000.0: 60
Number of HSP's gapped: 60
Number of HSP's successfully gapped: 60
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)

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