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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005091                                                              19     665
NP_653080                                                              19     665
XP_005267292                                                           19     665
XP_005267291                                                           19     665
XP_005267290                                                           19     665
NP_055206                                                              17    4312
NP_001360                                                              15    9605
NP_109592                                                              15    9605
NP_002839                                                              15    9605
NP_065924                                                              15    9605
XP_005248319                                                           15    9605
XP_005265408                                                           15    9605

>NP_005091
          Length = 1782

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    EYETAVTVS 9
            E+ETAVTVS
Sbjct: 1359 EHETAVTVS 1367



>NP_653080
          Length = 1684

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    EYETAVTVS 9
            E+ETAVTVS
Sbjct: 1261 EHETAVTVS 1269



>XP_005267292
          Length = 1677

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    EYETAVTVS 9
            E+ETAVTVS
Sbjct: 1254 EHETAVTVS 1262



>XP_005267291
          Length = 1684

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    EYETAVTVS 9
            E+ETAVTVS
Sbjct: 1261 EHETAVTVS 1269



>XP_005267290
          Length = 1782

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    EYETAVTVS 9
            E+ETAVTVS
Sbjct: 1359 EHETAVTVS 1367



>NP_055206
          Length = 319

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  EYETAVTV 8
          EYE AVT+
Sbjct: 32 EYEAAVTL 39



>NP_001360
          Length = 4624

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1    EYETAVTV 8
            +YET +T+
Sbjct: 1885 KYETLITI 1892



>NP_109592
          Length = 1216

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   YETAVTVS 9
           YE A TVS
Sbjct: 581 YEIAATVS 588



>NP_002839
          Length = 1188

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   YETAVTVS 9
           YE A TVS
Sbjct: 581 YEIAATVS 588



>NP_065924
          Length = 716

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   EYETAVTVS 9
           +YE  +TVS
Sbjct: 591 DYEVCLTVS 599



>XP_005248319
          Length = 4609

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1    EYETAVTV 8
            +YET +T+
Sbjct: 1870 KYETLITI 1877



>XP_005265408
          Length = 716

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   EYETAVTVS 9
           +YE  +TVS
Sbjct: 591 DYEVCLTVS 599



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.294    0.107    0.253 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 356,960
Number of extensions: 557
Number of successful extensions: 12
Number of sequences better than 10000.0: 12
Number of HSP's gapped: 12
Number of HSP's successfully gapped: 12
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.1 bits)
S2: 28 (15.4 bits)