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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_689957                                                              17    3301
NP_001264214                                                           17    3301
XP_006718858                                                           17    3301
NP_001092909                                                           15    9605
NP_001607                                                              15    9605
NP_001265509                                                           15    9605
NP_001265508                                                           15    9605
XP_006710647                                                           15    9605

>NP_689957
          Length = 2213

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   EVVTVITLA 9
           E VTV+T+A
Sbjct: 916 EAVTVVTIA 924



>NP_001264214
          Length = 1437

 Score = 16.9 bits (32), Expect =  3301
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   EVVTVITLA 9
           EVVTV TLA
Sbjct: 595 EVVTVNTLA 603



>XP_006718858
          Length = 913

 Score = 16.9 bits (32), Expect =  3301
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  EVVTVITLA 9
          EVVTV TLA
Sbjct: 71 EVVTVNTLA 79



>NP_001092909
          Length = 1008

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   VVTVITLA 9
           VVTV+T++
Sbjct: 567 VVTVVTIS 574



>NP_001607
          Length = 513

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   EVVTVITL 8
           ++VTV+T+
Sbjct: 493 DIVTVVTM 500



>NP_001265509
          Length = 405

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   EVVTVITL 8
           ++VTV+T+
Sbjct: 385 DIVTVVTM 392



>NP_001265508
          Length = 513

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   EVVTVITL 8
           ++VTV+T+
Sbjct: 493 DIVTVVTM 500



>XP_006710647
          Length = 509

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  VVTVITLA 9
          VVTV+T++
Sbjct: 62 VVTVVTIS 69



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.322    0.133    0.304 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 279,812
Number of extensions: 296
Number of successful extensions: 8
Number of sequences better than 10000.0: 8
Number of HSP's gapped: 8
Number of HSP's successfully gapped: 8
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)