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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005091                                                              18    1135
NP_653080                                                              18    1135
XP_005267292                                                           18    1135
XP_005267291                                                           18    1135
XP_005267290                                                           18    1135
NP_002801                                                              16    5631
XP_006711527                                                           16    5631
XP_005245411                                                           16    5631
NP_001035194                                                           16    5631
NP_057366                                                              16    7355
NP_001186781                                                           16    7355
NP_001186785                                                           16    7355
NP_001186782                                                           16    7355
NP_001186784                                                           16    7355
NP_056234                                                              15    9605
XP_005274542                                                           15    9605

>NP_005091
          Length = 1782

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   EVVSEKLTA 9
           EV SEKLTA
Sbjct: 344 EVASEKLTA 352



>NP_653080
          Length = 1684

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   EVVSEKLTA 9
           EV SEKLTA
Sbjct: 246 EVASEKLTA 254



>XP_005267292
          Length = 1677

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   EVVSEKLTA 9
           EV SEKLTA
Sbjct: 239 EVASEKLTA 247



>XP_005267291
          Length = 1684

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   EVVSEKLTA 9
           EV SEKLTA
Sbjct: 246 EVASEKLTA 254



>XP_005267290
          Length = 1782

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   EVVSEKLTA 9
           EV SEKLTA
Sbjct: 344 EVASEKLTA 352



>NP_002801
          Length = 377

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   EVVSEKLTA 9
           EV +EKLTA
Sbjct: 147 EVNTEKLTA 155



>XP_006711527
          Length = 333

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   EVVSEKLTA 9
           EV +EKLTA
Sbjct: 100 EVNTEKLTA 108



>XP_005245411
          Length = 380

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   EVVSEKLTA 9
           EV +EKLTA
Sbjct: 147 EVNTEKLTA 155



>NP_001035194
          Length = 4493

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    EVVSEKLT 8
            EVV EK+T
Sbjct: 4270 EVVKEKIT 4277



>NP_057366
          Length = 227

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   EVVSEKLTA 9
           EV+ ++LTA
Sbjct: 119 EVIKQRLTA 127



>NP_001186781
          Length = 187

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  EVVSEKLTA 9
          EV+ ++LTA
Sbjct: 79 EVIKQRLTA 87



>NP_001186785
          Length = 157

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  EVVSEKLTA 9
          EV+ ++LTA
Sbjct: 49 EVIKQRLTA 57



>NP_001186782
          Length = 157

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  EVVSEKLTA 9
          EV+ ++LTA
Sbjct: 49 EVIKQRLTA 57



>NP_001186784
          Length = 157

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  EVVSEKLTA 9
          EV+ ++LTA
Sbjct: 49 EVIKQRLTA 57



>NP_056234
          Length = 2828

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    VSEKLTA 9
            +SEK+TA
Sbjct: 2548 ISEKITA 2554



>XP_005274542
          Length = 2853

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    VSEKLTA 9
            +SEK+TA
Sbjct: 2573 ISEKITA 2579



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.294    0.111    0.240 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 368,832
Number of extensions: 393
Number of successful extensions: 16
Number of sequences better than 10000.0: 16
Number of HSP's gapped: 16
Number of HSP's successfully gapped: 16
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.0 bits)
S2: 28 (15.4 bits)