Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_006197                                                              19     665
XP_006714104                                                           19     665
XP_005265800                                                           19     665
XP_006714103                                                           19     665
XP_006714102                                                           19     665
NP_001005178                                                           17    3301
NP_055031                                                              16    5631
NP_001136065                                                           16    5631
NP_001136066                                                           16    5631
NP_002799                                                              16    5631
NP_001265637                                                           16    5631
NP_001265638                                                           16    5631
NP_002175                                                              16    7355
NP_786943                                                              16    7355
NP_001177910                                                           16    7355
NP_009162                                                              16    7355
NP_060033                                                              16    7355
XP_005248553                                                           16    7355
XP_005265295                                                           16    7355
NP_036292                                                              15    9605
NP_001265645                                                           15    9605
NP_060009                                                              15    9605
XP_006721125                                                           15    9605
XP_005266987                                                           15    9605
XP_006715521                                                           15    9605
NP_060422                                                              15    9605
>NP_006197
          Length = 1089

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  EVVQLNSSF 9
          +VVQLNSSF
Sbjct: 37 KVVQLNSSF 45
>XP_006714104
          Length = 832

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  EVVQLNSSF 9
          +VVQLNSSF
Sbjct: 62 KVVQLNSSF 70
>XP_005265800
          Length = 1089

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  EVVQLNSSF 9
          +VVQLNSSF
Sbjct: 37 KVVQLNSSF 45
>XP_006714103
          Length = 1111

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  EVVQLNSSF 9
          +VVQLNSSF
Sbjct: 59 KVVQLNSSF 67
>XP_006714102
          Length = 1114

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  EVVQLNSSF 9
          +VVQLNSSF
Sbjct: 62 KVVQLNSSF 70
>NP_001005178
          Length = 320

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  EVVQLNSSF 9
          E +QLNS+F
Sbjct: 3  ETLQLNSTF 11
>NP_055031
          Length = 414

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1  EVVQLNSS 8
          EVVQLN S
Sbjct: 37 EVVQLNLS 44
>NP_001136065
          Length = 414

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1  EVVQLNSS 8
          EVVQLN S
Sbjct: 37 EVVQLNLS 44
>NP_001136066
          Length = 314

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1  EVVQLNSS 8
          EVVQLN S
Sbjct: 37 EVVQLNLS 44
>NP_002799
          Length = 908

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   VQLNSSF 9
           VQLNS+F
Sbjct: 324 VQLNSNF 330
>NP_001265637
          Length = 778

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   VQLNSSF 9
           VQLNS+F
Sbjct: 194 VQLNSNF 200
>NP_001265638
          Length = 749

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   VQLNSSF 9
           VQLNS+F
Sbjct: 165 VQLNSNF 171
>NP_002175
          Length = 918

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  VVQLNSSF 9
          VVQL+S+F
Sbjct: 37 VVQLHSNF 44
>NP_786943
          Length = 329

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  VVQLNSSF 9
          VVQL+S+F
Sbjct: 37 VVQLHSNF 44
>NP_001177910
          Length = 857

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  VVQLNSSF 9
          VVQL+S+F
Sbjct: 37 VVQLHSNF 44
>NP_009162
          Length = 642

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   EVVQLNSSF 9
           E++Q+N S+
Sbjct: 184 EIIQMNKSW 192
>NP_060033
          Length = 739

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   QLNSSF 9
           QLNSSF
Sbjct: 135 QLNSSF 140
>XP_005248553
          Length = 333

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  VVQLNSSF 9
          VVQL+S+F
Sbjct: 37 VVQLHSNF 44
>XP_005265295
          Length = 711

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   QLNSSF 9
           QLNSSF
Sbjct: 107 QLNSSF 112
>NP_036292
          Length = 621

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   VVQLNSSF 9
           V++LN+SF
Sbjct: 603 VLELNASF 610
>NP_001265645
          Length = 621

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   VVQLNSSF 9
           V++LN+SF
Sbjct: 603 VLELNASF 610
>NP_060009
          Length = 4116

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1    EVVQLNS 7
            E++Q+NS
Sbjct: 1820 EIIQMNS 1826
>XP_006721125
          Length = 4070

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1    EVVQLNS 7
            E++Q+NS
Sbjct: 1774 EIIQMNS 1780
>XP_005266987
          Length = 597

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   VVQLNSSF 9
           V++LN+SF
Sbjct: 579 VLELNASF 586
>XP_006715521
          Length = 621

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   VVQLNSSF 9
           V++LN+SF
Sbjct: 603 VLELNASF 610
>NP_060422
          Length = 689

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   EVVQLNSSF 9
           EVV+L S+F
Sbjct: 629 EVVELASAF 637
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.311    0.124    0.295 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 329,563
Number of extensions: 332
Number of successful extensions: 26
Number of sequences better than 10000.0: 26
Number of HSP's gapped: 26
Number of HSP's successfully gapped: 26
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)

CANCERTOPE    |     Raghava's Group    |     IMTECH    |     CSIR    |     CRDD    |     GPSR Package