Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001135941                                                           18    1135
NP_001154819                                                           18    1482
NP_001154817                                                           18    1482
NP_060693                                                              18    1482
NP_001154818                                                           18    1482
NP_001154816                                                           18    1482
NP_055921                                                              18    1482
XP_006713125                                                           18    1482
XP_006713124                                                           18    1482
XP_005255896                                                           18    1935
XP_005255895                                                           18    1935
NP_001073961                                                           18    1935
NP_000966                                                              17    2528
NP_001186731                                                           17    2528
NP_001159694                                                           17    2528
XP_005274182                                                           17    2528
NP_001549                                                              17    3301
XP_006718896                                                           17    3301
NP_001034302                                                           16    5631
NP_001099007                                                           16    5631
XP_006712793                                                           16    5631
NP_001367                                                              16    7355
NP_001398                                                              16    7355
NP_001230396                                                           16    7355
NP_000701                                                              16    7355
NP_061834                                                              16    7355
NP_689530                                                              16    7355
NP_065810                                                              16    7355
NP_001001895                                                           16    7355
XP_006724076                                                           16    7355
XP_006720684                                                           16    7355
XP_006711547                                                           16    7355
XP_006711545                                                           16    7355
XP_006711544                                                           16    7355
NP_005895                                                              15    9605
NP_001177750                                                           15    9605
NP_001091995                                                           15    9605
NP_001399                                                              15    9605
NP_060674                                                              15    9605
XP_005266401                                                           15    9605
XP_005266400                                                           15    9605
XP_006710468                                                           15    9605
XP_005270637                                                           15    9605
XP_006710375                                                           15    9605
XP_006710374                                                           15    9605
XP_005270464                                                           15    9605
XP_005270463                                                           15    9605
XP_005270462                                                           15    9605
XP_006710373                                                           15    9605
XP_005270461                                                           15    9605
NP_443724                                                              15    9605
NP_001005289                                                           15    9605
NP_001273615                                                           15    9605
>NP_001135941
          Length = 187

 Score = 18.5 bits (36), Expect =  1135
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  EVTRTGCLG 9
          ++T TGC+G
Sbjct: 4  DLTETGCMG 12
>NP_001154819
          Length = 572

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   TRTGCLG 9
           TRTGC G
Sbjct: 433 TRTGCYG 439
>NP_001154817
          Length = 663

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   TRTGCLG 9
           TRTGC G
Sbjct: 524 TRTGCYG 530
>NP_060693
          Length = 623

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   TRTGCLG 9
           TRTGC G
Sbjct: 484 TRTGCYG 490
>NP_001154818
          Length = 652

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   TRTGCLG 9
           TRTGC G
Sbjct: 513 TRTGCYG 519
>NP_001154816
          Length = 664

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   TRTGCLG 9
           TRTGC G
Sbjct: 525 TRTGCYG 531
>NP_055921
          Length = 1467

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   TRTGCLG 9
           TR GCLG
Sbjct: 816 TRAGCLG 822
>XP_006713125
          Length = 1467

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   TRTGCLG 9
           TR GCLG
Sbjct: 816 TRAGCLG 822
>XP_006713124
          Length = 1467

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   TRTGCLG 9
           TR GCLG
Sbjct: 816 TRAGCLG 822
>XP_005255896
          Length = 296

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EVTRTGCLG 9
           E T+T CLG
Sbjct: 112 EETKTACLG 120
>XP_005255895
          Length = 395

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EVTRTGCLG 9
           E T+T CLG
Sbjct: 211 EETKTACLG 219
>NP_001073961
          Length = 395

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EVTRTGCLG 9
           E T+T CLG
Sbjct: 211 EETKTACLG 219
>NP_000966
          Length = 178

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   RTGCLG 9
           RTGC+G
Sbjct: 147 RTGCIG 152
>NP_001186731
          Length = 177

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   RTGCLG 9
           RTGC+G
Sbjct: 146 RTGCIG 151
>NP_001159694
          Length = 950

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  EVTRTGCLG 9
          +V RTGC G
Sbjct: 87 QVPRTGCPG 95
>XP_005274182
          Length = 966

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EVTRTGCLG 9
           +V RTGC G
Sbjct: 103 QVPRTGCPG 111
>NP_001549
          Length = 578

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   EVTRTGCL 8
           + T+TGCL
Sbjct: 456 KATKTGCL 463
>XP_006718896
          Length = 558

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   EVTRTGCL 8
           + T+TGCL
Sbjct: 436 KATKTGCL 443
>NP_001034302
          Length = 425

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 3   TRTGC 7
           TRTGC
Sbjct: 234 TRTGC 238
>NP_001099007
          Length = 1687

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   TRTGCLG 9
           +RTGC G
Sbjct: 855 SRTGCQG 861
>XP_006712793
          Length = 1887

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3    TRTGCLG 9
            +RTGC G
Sbjct: 1055 SRTGCQG 1061
>NP_001367
          Length = 4646

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    EVTRTGCLG 9
            ++TR  CLG
Sbjct: 2448 DLTRLRCLG 2456
>NP_001398
          Length = 3312

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1    EVTRTGC 7
            EVT +GC
Sbjct: 2116 EVTASGC 2122
>NP_001230396
          Length = 526

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   RTGCLG 9
           RTGC G
Sbjct: 286 RTGCRG 291
>NP_000701
          Length = 353

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EVTRTGCLG 9
           EV+RT C G
Sbjct: 234 EVSRTRCGG 242
>NP_061834
          Length = 661

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   RTGCLG 9
           RTGC G
Sbjct: 324 RTGCRG 329
>NP_689530
          Length = 294

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4  RTGCLG 9
          RTGC G
Sbjct: 51 RTGCSG 56
>NP_065810
          Length = 4700

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 4   RTGCL 8
           RTGCL
Sbjct: 886 RTGCL 890
>NP_001001895
          Length = 623

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   RTGCLG 9
           RTGC G
Sbjct: 286 RTGCRG 291
>XP_006724076
          Length = 642

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   RTGCLG 9
           RTGC G
Sbjct: 305 RTGCRG 310
>XP_006720684
          Length = 4713

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 4   RTGCL 8
           RTGCL
Sbjct: 899 RTGCL 903
>XP_006711547
          Length = 572

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 4  RTGCL 8
          RTGCL
Sbjct: 63 RTGCL 67
>XP_006711545
          Length = 247

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 2   VTRTGC 7
           VTR GC
Sbjct: 186 VTRAGC 191
>XP_006711544
          Length = 251

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 2   VTRTGC 7
           VTR GC
Sbjct: 186 VTRAGC 191
>NP_005895
          Length = 426

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 3   TRTGCL 8
           TRT CL
Sbjct: 121 TRTACL 126
>NP_001177750
          Length = 425

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 3   TRTGCL 8
           TRT CL
Sbjct: 121 TRTACL 126
>NP_001091995
          Length = 683

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 3   TRTGCL 8
           TRT CL
Sbjct: 584 TRTSCL 589
>NP_001399
          Length = 2923

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 1    EVTRTGC 7
            EVT  GC
Sbjct: 1963 EVTTNGC 1969
>NP_060674
          Length = 682

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 3   TRTGCL 8
           TRT CL
Sbjct: 583 TRTSCL 588
>XP_005266401
          Length = 635

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 3   TRTGCL 8
           TRT CL
Sbjct: 536 TRTSCL 541
>XP_005266400
          Length = 677

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 3   TRTGCL 8
           TRT CL
Sbjct: 578 TRTSCL 583
>XP_006710468
          Length = 2916

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 1    EVTRTGC 7
            EVT  GC
Sbjct: 1963 EVTTNGC 1969
>XP_005270637
          Length = 2921

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 1    EVTRTGC 7
            EVT  GC
Sbjct: 1963 EVTTNGC 1969
>XP_006710375
          Length = 309

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   EVTRTGCLG 9
           E+T T C+G
Sbjct: 292 EITDTLCVG 300
>XP_006710374
          Length = 309

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   EVTRTGCLG 9
           E+T T C+G
Sbjct: 292 EITDTLCVG 300
>XP_005270464
          Length = 365

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   EVTRTGCLG 9
           E+T T C+G
Sbjct: 348 EITDTLCVG 356
>XP_005270463
          Length = 402

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   EVTRTGCLG 9
           E+T T C+G
Sbjct: 385 EITDTLCVG 393
>XP_005270462
          Length = 460

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   EVTRTGCLG 9
           E+T T C+G
Sbjct: 443 EITDTLCVG 451
>XP_006710373
          Length = 460

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   EVTRTGCLG 9
           E+T T C+G
Sbjct: 443 EITDTLCVG 451
>XP_005270461
          Length = 460

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   EVTRTGCLG 9
           E+T T C+G
Sbjct: 443 EITDTLCVG 451
>NP_443724
          Length = 460

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   EVTRTGCLG 9
           E+T T C+G
Sbjct: 443 EITDTLCVG 451
>NP_001005289
          Length = 320

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   EVTRTGCL 8
           E+T  GCL
Sbjct: 99  EITFPGCL 106
>NP_001273615
          Length = 634

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 3   TRTGCL 8
           TRT CL
Sbjct: 535 TRTSCL 540
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.321    0.141    0.455 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 590,973
Number of extensions: 715
Number of successful extensions: 53
Number of sequences better than 10000.0: 53
Number of HSP's gapped: 53
Number of HSP's successfully gapped: 53
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)

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