Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000305                                                              20     390
XP_006717989                                                           20     390
NP_001035114                                                           18    1935
NP_001276324                                                           17    3301
NP_003313                                                              17    3301
NP_813977                                                              17    3301
NP_003311                                                              17    3301
XP_006718366                                                           17    3301
XP_005253166                                                           17    3301
NP_000247                                                              17    4312
NP_663771                                                              17    4312
XP_006718301                                                           17    4312
NP_001258931                                                           16    5631
NP_212132                                                              16    5631
NP_001258929                                                           16    5631
NP_001258930                                                           16    5631
NP_001258928                                                           16    5631
NP_079410                                                              16    5631
XP_005256233                                                           16    5631
XP_005256232                                                           16    5631
XP_005256229                                                           16    5631
XP_005256228                                                           16    5631
XP_005256227                                                           16    5631
XP_006721346                                                           16    5631
XP_005256226                                                           16    5631
XP_006721345                                                           16    5631
XP_006721344                                                           16    5631
XP_006721343                                                           16    5631
XP_005256225                                                           16    5631
XP_005271350                                                           16    5631
XP_005271349                                                           16    5631
NP_001866                                                              16    7355
NP_001156002                                                           16    7355
NP_001161710                                                           16    7355
NP_001161709                                                           16    7355
NP_001161708                                                           16    7355
NP_001116105                                                           16    7355
NP_009298                                                              16    7355
NP_005149                                                              16    7355
NP_001161711                                                           16    7355
XP_005245145                                                           16    7355
XP_005271262                                                           16    7355
XP_006710965                                                           16    7355
XP_006710964                                                           16    7355
XP_005271261                                                           16    7355
NP_001129472                                                           16    7355
NP_078952                                                              16    7355
NP_001003712                                                           15    9605
NP_065892                                                              15    9605
NP_115784                                                              15    9605
NP_001138537                                                           15    9605
NP_006548                                                              15    9605
NP_001120936                                                           15    9605
NP_006495                                                              15    9605
NP_870987                                                              15    9605
NP_001124337                                                           15    9605
XP_005268680                                                           15    9605
XP_006719287                                                           15    9605
XP_005268679                                                           15    9605
XP_005268678                                                           15    9605
XP_006719286                                                           15    9605
XP_006719285                                                           15    9605
XP_006718528                                                           15    9605
XP_006717995                                                           15    9605
XP_005252749                                                           15    9605
XP_005252748                                                           15    9605
XP_005251411                                                           15    9605
XP_005251409                                                           15    9605
XP_005251408                                                           15    9605
XP_006716780                                                           15    9605
NP_569119                                                              15    9605
>NP_000305
          Length = 403

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   EVSTRDKKG 9
           EV TRDKKG
Sbjct: 157 EVRTRDKKG 165
>XP_006717989
          Length = 388

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   EVSTRDKKG 9
           EV TRDKKG
Sbjct: 142 EVRTRDKKG 150
>NP_001035114
          Length = 197

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  EVSTRDKKG 9
          EVS+RD KG
Sbjct: 45 EVSSRDGKG 53
>NP_001276324
          Length = 489

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   TRDKKG 9
           TRDKKG
Sbjct: 257 TRDKKG 262
>NP_003313
          Length = 542

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   TRDKKG 9
           TRDKKG
Sbjct: 310 TRDKKG 315
>NP_813977
          Length = 506

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   TRDKKG 9
           TRDKKG
Sbjct: 270 TRDKKG 275
>NP_003311
          Length = 561

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   TRDKKG 9
           TRDKKG
Sbjct: 325 TRDKKG 330
>XP_006718366
          Length = 451

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   TRDKKG 9
           TRDKKG
Sbjct: 312 TRDKKG 317
>XP_005253166
          Length = 548

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   TRDKKG 9
           TRDKKG
Sbjct: 312 TRDKKG 317
>NP_000247
          Length = 1274

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   EVSTRDK 7
           EVST+DK
Sbjct: 240 EVSTKDK 246
>NP_663771
          Length = 471

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   EVSTRDKKG 9
           E+ T D+KG
Sbjct: 411 EIGTEDEKG 419
>XP_006718301
          Length = 1268

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   EVSTRDK 7
           EVST+DK
Sbjct: 240 EVSTKDK 246
>NP_001258931
          Length = 632

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   EVSTRDKKG 9
           E+ST ++KG
Sbjct: 423 EISTGERKG 431
>NP_212132
          Length = 2109

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   EVSTRDKKG 9
           E+ST ++KG
Sbjct: 423 EISTGERKG 431
>NP_001258929
          Length = 2100

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   EVSTRDKKG 9
           E+ST ++KG
Sbjct: 423 EISTGERKG 431
>NP_001258930
          Length = 2098

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   EVSTRDKKG 9
           E+ST ++KG
Sbjct: 423 EISTGERKG 431
>NP_001258928
          Length = 2129

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   EVSTRDKKG 9
           E+ST ++KG
Sbjct: 423 EISTGERKG 431
>NP_079410
          Length = 2881

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    EVSTRDKKG 9
            E  T DKKG
Sbjct: 2720 ESGTEDKKG 2728
>XP_005256233
          Length = 2391

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    EVSTRDKKG 9
            E  T DKKG
Sbjct: 2230 ESGTEDKKG 2238
>XP_005256232
          Length = 2424

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    EVSTRDKKG 9
            E  T DKKG
Sbjct: 2263 ESGTEDKKG 2271
>XP_005256229
          Length = 2881

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    EVSTRDKKG 9
            E  T DKKG
Sbjct: 2720 ESGTEDKKG 2728
>XP_005256228
          Length = 2882

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    EVSTRDKKG 9
            E  T DKKG
Sbjct: 2721 ESGTEDKKG 2729
>XP_005256227
          Length = 2897

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    EVSTRDKKG 9
            E  T DKKG
Sbjct: 2736 ESGTEDKKG 2744
>XP_006721346
          Length = 2898

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    EVSTRDKKG 9
            E  T DKKG
Sbjct: 2737 ESGTEDKKG 2745
>XP_005256226
          Length = 2898

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    EVSTRDKKG 9
            E  T DKKG
Sbjct: 2737 ESGTEDKKG 2745
>XP_006721345
          Length = 2898

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    EVSTRDKKG 9
            E  T DKKG
Sbjct: 2737 ESGTEDKKG 2745
>XP_006721344
          Length = 2898

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    EVSTRDKKG 9
            E  T DKKG
Sbjct: 2737 ESGTEDKKG 2745
>XP_006721343
          Length = 2898

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    EVSTRDKKG 9
            E  T DKKG
Sbjct: 2737 ESGTEDKKG 2745
>XP_005256225
          Length = 2898

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    EVSTRDKKG 9
            E  T DKKG
Sbjct: 2737 ESGTEDKKG 2745
>XP_005271350
          Length = 1854

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   EVSTRDKKG 9
           E+ST ++KG
Sbjct: 423 EISTGERKG 431
>XP_005271349
          Length = 2131

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   EVSTRDKKG 9
           E+ST ++KG
Sbjct: 423 EISTGERKG 431
>NP_001866
          Length = 1500

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   EVSTRDKK 8
           EVST+D K
Sbjct: 203 EVSTKDVK 210
>NP_001156002
          Length = 756

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   EVSTRD 6
           EVSTRD
Sbjct: 494 EVSTRD 499
>NP_001161710
          Length = 1058

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   TRDKKG 9
           TRD+KG
Sbjct: 624 TRDRKG 629
>NP_001161709
          Length = 1079

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   TRDKKG 9
           TRD+KG
Sbjct: 645 TRDRKG 650
>NP_001161708
          Length = 1161

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   TRDKKG 9
           TRD+KG
Sbjct: 624 TRDRKG 629
>NP_001116105
          Length = 1506

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   EVSTRDKK 8
           EVST+D K
Sbjct: 209 EVSTKDVK 216
>NP_009298
          Length = 1182

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   TRDKKG 9
           TRD+KG
Sbjct: 645 TRDRKG 650
>NP_005149
          Length = 1167

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   TRDKKG 9
           TRD+KG
Sbjct: 630 TRDRKG 635
>NP_001161711
          Length = 1043

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   TRDKKG 9
           TRD+KG
Sbjct: 609 TRDRKG 614
>XP_005245145
          Length = 1146

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   TRDKKG 9
           TRD+KG
Sbjct: 609 TRDRKG 614
>XP_005271262
          Length = 603

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   EVSTRD 6
           EVSTRD
Sbjct: 291 EVSTRD 296
>XP_006710965
          Length = 770

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   EVSTRD 6
           EVSTRD
Sbjct: 494 EVSTRD 499
>XP_006710964
          Length = 781

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   EVSTRD 6
           EVSTRD
Sbjct: 494 EVSTRD 499
>XP_005271261
          Length = 806

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   EVSTRD 6
           EVSTRD
Sbjct: 494 EVSTRD 499
>NP_001129472
          Length = 1064

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   TRDKKG 9
           TRD+KG
Sbjct: 630 TRDRKG 635
>NP_078952
          Length = 645

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   EVSTRD 6
           EVSTRD
Sbjct: 383 EVSTRD 388
>NP_001003712
          Length = 847

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   STRDKKG 9
           STR KKG
Sbjct: 776 STRRKKG 782
>NP_065892
          Length = 889

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   STRDKKG 9
           STR KKG
Sbjct: 818 STRRKKG 824
>NP_115784
          Length = 1483

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    EVSTRDKKG 9
            EV+TR +KG
Sbjct: 1061 EVATRLQKG 1069
>NP_001138537
          Length = 900

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VSTRDKK 8
           +STRD+K
Sbjct: 751 LSTRDRK 757
>NP_006548
          Length = 226

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   STRDKKG 9
           +T DKKG
Sbjct: 173 ATEDKKG 179
>NP_001120936
          Length = 3925

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    EVSTRDKKG 9
            E ST+ KKG
Sbjct: 3784 ESSTKRKKG 3792
>NP_006495
          Length = 700

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  VSTRDKK 8
          VST DKK
Sbjct: 79 VSTSDKK 85
>NP_870987
          Length = 561

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   STRDKKG 9
           +T DKKG
Sbjct: 173 ATEDKKG 179
>NP_001124337
          Length = 226

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   STRDKKG 9
           +T DKKG
Sbjct: 173 ATEDKKG 179
>XP_005268680
          Length = 847

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   STRDKKG 9
           STR KKG
Sbjct: 776 STRRKKG 782
>XP_006719287
          Length = 847

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   STRDKKG 9
           STR KKG
Sbjct: 776 STRRKKG 782
>XP_005268679
          Length = 886

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   STRDKKG 9
           STR KKG
Sbjct: 815 STRRKKG 821
>XP_005268678
          Length = 889

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   STRDKKG 9
           STR KKG
Sbjct: 818 STRRKKG 824
>XP_006719286
          Length = 909

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   STRDKKG 9
           STR KKG
Sbjct: 838 STRRKKG 844
>XP_006719285
          Length = 912

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   STRDKKG 9
           STR KKG
Sbjct: 841 STRRKKG 847
>XP_006718528
          Length = 161

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   TRDKKG 9
           TRDK+G
Sbjct: 146 TRDKQG 151
>XP_006717995
          Length = 699

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  VSTRDKK 8
          VST DKK
Sbjct: 79 VSTSDKK 85
>XP_005252749
          Length = 700

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  VSTRDKK 8
          VST DKK
Sbjct: 79 VSTSDKK 85
>XP_005252748
          Length = 700

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  VSTRDKK 8
          VST DKK
Sbjct: 79 VSTSDKK 85
>XP_005251411
          Length = 226

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   STRDKKG 9
           +T DKKG
Sbjct: 173 ATEDKKG 179
>XP_005251409
          Length = 561

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   STRDKKG 9
           +T DKKG
Sbjct: 173 ATEDKKG 179
>XP_005251408
          Length = 561

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   STRDKKG 9
           +T DKKG
Sbjct: 173 ATEDKKG 179
>XP_006716780
          Length = 561

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   STRDKKG 9
           +T DKKG
Sbjct: 173 ATEDKKG 179
>NP_569119
          Length = 642

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  VSTRDKK 8
          VST DKK
Sbjct: 21 VSTSDKK 27
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.296    0.119    0.299 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 460,575
Number of extensions: 578
Number of successful extensions: 71
Number of sequences better than 10000.0: 71
Number of HSP's gapped: 71
Number of HSP's successfully gapped: 71
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.0 bits)
S2: 28 (15.4 bits)

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