Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              21     229
NP_001075109                                                           21     229
NP_997639                                                              18    1482
NP_002017                                                              18    1482
NP_997643                                                              18    1482
NP_997647                                                              18    1482
NP_997641                                                              18    1482
XP_005246474                                                           18    1482
XP_005246473                                                           18    1482
XP_005246472                                                           18    1482
XP_005246471                                                           18    1482
XP_005246470                                                           18    1482
XP_005246469                                                           18    1482
XP_005246468                                                           18    1482
XP_005246467                                                           18    1482
XP_005246466                                                           18    1482
XP_005246465                                                           18    1482
XP_005246464                                                           18    1482
XP_005246463                                                           18    1482
XP_005246462                                                           18    1482
XP_005246461                                                           18    1482
XP_005246460                                                           18    1482
XP_005246459                                                           18    1482
XP_005246458                                                           18    1482
XP_005246457                                                           18    1482
XP_005246456                                                           18    1482
XP_005246455                                                           18    1482
XP_005246454                                                           18    1482
NP_055906                                                              17    2528
XP_006720146                                                           17    2528
XP_005267508                                                           17    2528
NP_006764                                                              16    7355
NP_065801                                                              15    9605
NP_006604                                                              15    9605
NP_872332                                                              15    9605
XP_006720125                                                           15    9605
XP_005267432                                                           15    9605
XP_005267431                                                           15    9605
XP_005267430                                                           15    9605
>NP_008835
          Length = 4128

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    EVSQFDFST 9
            E+SQFDFST
Sbjct: 2039 EMSQFDFST 2047
>NP_001075109
          Length = 4097

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    EVSQFDFST 9
            E+SQFDFST
Sbjct: 2039 EMSQFDFST 2047
>NP_997639
          Length = 2176

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   EVSQFDFST 9
           EV++FDF+T
Sbjct: 691 EVTRFDFTT 699
>NP_002017
          Length = 2355

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   EVSQFDFST 9
           EV++FDF+T
Sbjct: 691 EVTRFDFTT 699
>NP_997643
          Length = 2330

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   EVSQFDFST 9
           EV++FDF+T
Sbjct: 691 EVTRFDFTT 699
>NP_997647
          Length = 2477

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   EVSQFDFST 9
           EV++FDF+T
Sbjct: 691 EVTRFDFTT 699
>NP_997641
          Length = 2296

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   EVSQFDFST 9
           EV++FDF+T
Sbjct: 691 EVTRFDFTT 699
>XP_005246474
          Length = 2240

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   EVSQFDFST 9
           EV++FDF+T
Sbjct: 691 EVTRFDFTT 699
>XP_005246473
          Length = 2246

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   EVSQFDFST 9
           EV++FDF+T
Sbjct: 691 EVTRFDFTT 699
>XP_005246472
          Length = 2265

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   EVSQFDFST 9
           EV++FDF+T
Sbjct: 691 EVTRFDFTT 699
>XP_005246471
          Length = 2266

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   EVSQFDFST 9
           EV++FDF+T
Sbjct: 691 EVTRFDFTT 699
>XP_005246470
          Length = 2267

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   EVSQFDFST 9
           EV++FDF+T
Sbjct: 691 EVTRFDFTT 699
>XP_005246469
          Length = 2271

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   EVSQFDFST 9
           EV++FDF+T
Sbjct: 691 EVTRFDFTT 699
>XP_005246468
          Length = 2273

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   EVSQFDFST 9
           EV++FDF+T
Sbjct: 691 EVTRFDFTT 699
>XP_005246467
          Length = 2337

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   EVSQFDFST 9
           EV++FDF+T
Sbjct: 691 EVTRFDFTT 699
>XP_005246466
          Length = 2361

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   EVSQFDFST 9
           EV++FDF+T
Sbjct: 691 EVTRFDFTT 699
>XP_005246465
          Length = 2362

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   EVSQFDFST 9
           EV++FDF+T
Sbjct: 691 EVTRFDFTT 699
>XP_005246464
          Length = 2363

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   EVSQFDFST 9
           EV++FDF+T
Sbjct: 691 EVTRFDFTT 699
>XP_005246463
          Length = 2386

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   EVSQFDFST 9
           EV++FDF+T
Sbjct: 691 EVTRFDFTT 699
>XP_005246462
          Length = 2387

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   EVSQFDFST 9
           EV++FDF+T
Sbjct: 691 EVTRFDFTT 699
>XP_005246461
          Length = 2392

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   EVSQFDFST 9
           EV++FDF+T
Sbjct: 691 EVTRFDFTT 699
>XP_005246460
          Length = 2393

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   EVSQFDFST 9
           EV++FDF+T
Sbjct: 691 EVTRFDFTT 699
>XP_005246459
          Length = 2421

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   EVSQFDFST 9
           EV++FDF+T
Sbjct: 691 EVTRFDFTT 699
>XP_005246458
          Length = 2427

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   EVSQFDFST 9
           EV++FDF+T
Sbjct: 691 EVTRFDFTT 699
>XP_005246457
          Length = 2446

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   EVSQFDFST 9
           EV++FDF+T
Sbjct: 691 EVTRFDFTT 699
>XP_005246456
          Length = 2452

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   EVSQFDFST 9
           EV++FDF+T
Sbjct: 691 EVTRFDFTT 699
>XP_005246455
          Length = 2458

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   EVSQFDFST 9
           EV++FDF+T
Sbjct: 691 EVTRFDFTT 699
>XP_005246454
          Length = 2483

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   EVSQFDFST 9
           EV++FDF+T
Sbjct: 691 EVTRFDFTT 699
>NP_055906
          Length = 1720

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    EVSQFDFST 9
            ++S FDF+T
Sbjct: 1087 QISSFDFTT 1095
>XP_006720146
          Length = 1226

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    EVSQFDFST 9
            ++S FDF+T
Sbjct: 1087 QISSFDFTT 1095
>XP_005267508
          Length = 1773

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    EVSQFDFST 9
            ++S FDF+T
Sbjct: 1087 QISSFDFTT 1095
>NP_006764
          Length = 670

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VSQFDFS 8
           +S+FDFS
Sbjct: 548 LSEFDFS 554
>NP_065801
          Length = 1204

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   EVSQFDFST 9
           E S+FDF +
Sbjct: 420 EYSRFDFDS 428
>NP_006604
          Length = 217

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   SQFDFS 8
           +QFDFS
Sbjct: 165 AQFDFS 170
>NP_872332
          Length = 167

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   EVSQFDF 7
           + SQFDF
Sbjct: 104 DYSQFDF 110
>XP_006720125
          Length = 167

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   EVSQFDF 7
           + SQFDF
Sbjct: 104 DYSQFDF 110
>XP_005267432
          Length = 167

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   EVSQFDF 7
           + SQFDF
Sbjct: 104 DYSQFDF 110
>XP_005267431
          Length = 167

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   EVSQFDF 7
           + SQFDF
Sbjct: 104 DYSQFDF 110
>XP_005267430
          Length = 167

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   EVSQFDF 7
           + SQFDF
Sbjct: 104 DYSQFDF 110
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.316    0.126    0.331 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 420,834
Number of extensions: 497
Number of successful extensions: 39
Number of sequences better than 10000.0: 39
Number of HSP's gapped: 39
Number of HSP's successfully gapped: 39
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)

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