Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

• Home Page
• Cancer-specific
• Epitope Search
• Data Retrieval
• Browse Gene
• Browse Tissue
• Sequence Similarity
• Partially Personalized
• Submit a Protein
• Vaccine Target
• Proteome Data
• NGS data
• BLAST & Predict
• Fully Personalized
• Proteins Pair
• Proteome Pair
• Advanced Tools
• Epitope Mapping
• Cross Reactivity
• Important Information
• Acknowledgement
• Algorithm
• Help
• Developers
• Contact

Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004351                                                              20     390
NP_056073                                                              17    4312
NP_940865                                                              17    4312
XP_006717082                                                           17    4312
XP_006717081                                                           17    4312
XP_005264988                                                           17    4312
XP_005264986                                                           17    4312
XP_005264985                                                           17    4312
XP_006713076                                                           17    4312
NP_005387                                                              16    5631
NP_001274278                                                           16    7355
NP_056175                                                              16    7355
NP_001007534                                                           16    7355
NP_112224                                                              15    9605
NP_001274685                                                           15    9605
NP_001274679                                                           15    9605
NP_001274678                                                           15    9605
NP_001274680                                                           15    9605
NP_001274672                                                           15    9605
NP_001274673                                                           15    9605
NP_001274674                                                           15    9605
NP_004453                                                              15    9605
NP_001274677                                                           15    9605
NP_001274671                                                           15    9605
NP_001274676                                                           15    9605
NP_001074025                                                           15    9605
XP_005256532                                                           15    9605
XP_005253212                                                           15    9605
XP_005253211                                                           15    9605
XP_006716310                                                           15    9605
NP_659491                                                              15    9605

>NP_004351
          Length = 882

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   EVFKGSVMK 9
           EVFKGSVM+
Sbjct: 265 EVFKGSVME 273



>NP_056073
          Length = 1219

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    EVFKGSVMK 9
            E+FKG+ +K
Sbjct: 1170 ELFKGATLK 1178



>NP_940865
          Length = 563

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  EVFKGSVMK 9
          E+FKG+V +
Sbjct: 20 EIFKGAVFQ 28



>XP_006717082
          Length = 1151

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    EVFKGSVMK 9
            E+FKG+ +K
Sbjct: 1102 ELFKGATLK 1110



>XP_006717081
          Length = 1209

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    EVFKGSVMK 9
            E+FKG+ +K
Sbjct: 1160 ELFKGATLK 1168



>XP_005264988
          Length = 746

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   EVFKGSVMK 9
           E+FKG+V +
Sbjct: 145 EIFKGAVFQ 153



>XP_005264986
          Length = 801

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   EVFKGSVMK 9
           E+FKG+V +
Sbjct: 145 EIFKGAVFQ 153



>XP_005264985
          Length = 813

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   EVFKGSVMK 9
           E+FKG+V +
Sbjct: 145 EIFKGAVFQ 153



>XP_006713076
          Length = 814

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   EVFKGSVMK 9
           E+FKG+V +
Sbjct: 145 EIFKGAVFQ 153



>NP_005387
          Length = 470

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   EVFKGSV 7
           E+FKGS+
Sbjct: 391 EIFKGSL 397



>NP_001274278
          Length = 530

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  EVFKGS 6
          E+FKGS
Sbjct: 62 EIFKGS 67



>NP_056175
          Length = 1042

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   EVFKGSVMK 9
           +VF+GS+++
Sbjct: 987 DVFEGSIIR 995



>NP_001007534
          Length = 154

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   EVFKGSVM 8
           E+FKG+ M
Sbjct: 146 ELFKGTTM 153



>NP_112224
          Length = 1849

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    EVFKGSVMK 9
            E+FK  +MK
Sbjct: 994  EIFKKQLMK 1002



>NP_001274685
          Length = 250

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   EVFKGSV 7
           +VFKG+V
Sbjct: 141 QVFKGAV 147



>NP_001274679
          Length = 476

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   EVFKGSV 7
           +VFKG+V
Sbjct: 367 QVFKGAV 373



>NP_001274678
          Length = 353

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   EVFKGSV 7
           +VFKG+V
Sbjct: 244 QVFKGAV 250



>NP_001274680
          Length = 332

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   EVFKGSV 7
           +VFKG+V
Sbjct: 223 QVFKGAV 229



>NP_001274672
          Length = 417

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   EVFKGSV 7
           +VFKG+V
Sbjct: 308 QVFKGAV 314



>NP_001274673
          Length = 353

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   EVFKGSV 7
           +VFKG+V
Sbjct: 244 QVFKGAV 250



>NP_001274674
          Length = 353

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   EVFKGSV 7
           +VFKG+V
Sbjct: 244 QVFKGAV 250



>NP_004453
          Length = 417

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   EVFKGSV 7
           +VFKG+V
Sbjct: 308 QVFKGAV 314



>NP_001274677
          Length = 353

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   EVFKGSV 7
           +VFKG+V
Sbjct: 244 QVFKGAV 250



>NP_001274671
          Length = 417

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   EVFKGSV 7
           +VFKG+V
Sbjct: 308 QVFKGAV 314



>NP_001274676
          Length = 353

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   EVFKGSV 7
           +VFKG+V
Sbjct: 244 QVFKGAV 250



>NP_001074025
          Length = 552

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   EVFKGSVMK 9
           EVF G+V++
Sbjct: 462 EVFDGTVIR 470



>XP_005256532
          Length = 531

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   EVFKGSVMK 9
           EVF G+V++
Sbjct: 441 EVFDGTVIR 449



>XP_005253212
          Length = 1838

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   EVFKGSVMK 9
           E+FK  +MK
Sbjct: 951 EIFKKQLMK 959



>XP_005253211
          Length = 1881

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    EVFKGSVMK 9
            E+FK  +MK
Sbjct: 994  EIFKKQLMK 1002



>XP_006716310
          Length = 358

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   EVFKGSV 7
           +VFKG+V
Sbjct: 249 QVFKGAV 255



>NP_659491
          Length = 620

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   EVFKGSVMK 9
           EVF G+V++
Sbjct: 530 EVFDGTVIR 538



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.318    0.133    0.345 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 489,512
Number of extensions: 456
Number of successful extensions: 31
Number of sequences better than 10000.0: 31
Number of HSP's gapped: 31
Number of HSP's successfully gapped: 31
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)