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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_056078                                                              17    3301
NP_001167587                                                           17    3301
NP_001167588                                                           17    3301
XP_005254315                                                           17    3301
XP_005254314                                                           17    3301
XP_006720509                                                           17    3301
XP_005254313                                                           17    3301
XP_005254312                                                           17    3301
NP_001095841                                                           17    4312
NP_079231                                                              17    4312
XP_006724383                                                           17    4312
NP_116023                                                              16    7355
NP_694955                                                              16    7355
NP_001159698                                                           16    7355
XP_005251676                                                           16    7355
XP_005271958                                                           16    7355
NP_064592                                                              15    9605
NP_777596                                                              15    9605
NP_001121361                                                           15    9605
NP_057714                                                              15    9605
NP_001121362                                                           15    9605
NP_001239049                                                           15    9605
NP_055963                                                              15    9605
NP_001165422                                                           15    9605
NP_001239053                                                           15    9605
NP_001239051                                                           15    9605
NP_001239048                                                           15    9605
NP_001008271                                                           15    9605
XP_005254494                                                           15    9605
XP_005254493                                                           15    9605
XP_006720627                                                           15    9605
XP_005252384                                                           15    9605
XP_005250004                                                           15    9605
XP_005250003                                                           15    9605
XP_005247048                                                           15    9605
XP_005247047                                                           15    9605
NP_002144                                                              15    9605

>NP_056078
          Length = 3036

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    EVFFSDTSV 9
            + FFSDT +
Sbjct: 2488 DAFFSDTQI 2496



>NP_001167587
          Length = 3037

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    EVFFSDTSV 9
            + FFSDT +
Sbjct: 2489 DAFFSDTQI 2497



>NP_001167588
          Length = 2400

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    EVFFSDTSV 9
            + FFSDT +
Sbjct: 1852 DAFFSDTQI 1860



>XP_005254315
          Length = 2576

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    EVFFSDTSV 9
            + FFSDT +
Sbjct: 2489 DAFFSDTQI 2497



>XP_005254314
          Length = 2605

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    EVFFSDTSV 9
            + FFSDT +
Sbjct: 2489 DAFFSDTQI 2497



>XP_006720509
          Length = 2646

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    EVFFSDTSV 9
            + FFSDT +
Sbjct: 2489 DAFFSDTQI 2497



>XP_005254313
          Length = 3057

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    EVFFSDTSV 9
            + FFSDT +
Sbjct: 2488 DAFFSDTQI 2496



>XP_005254312
          Length = 3058

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    EVFFSDTSV 9
            + FFSDT +
Sbjct: 2489 DAFFSDTQI 2497



>NP_001095841
          Length = 684

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EVFFSDTSV 9
           +VFF D SV
Sbjct: 509 DVFFDDRSV 517



>NP_079231
          Length = 684

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EVFFSDTSV 9
           +VFF D SV
Sbjct: 509 DVFFDDRSV 517



>XP_006724383
          Length = 594

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EVFFSDTSV 9
           +VFF D SV
Sbjct: 419 DVFFDDRSV 427



>NP_116023
          Length = 1089

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   VFFSDTSV 9
           VFFSD+ V
Sbjct: 559 VFFSDSFV 566



>NP_694955
          Length = 986

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   EVFFSDTS 8
           E+F SD+S
Sbjct: 612 EIFISDSS 619



>NP_001159698
          Length = 960

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   EVFFSDTS 8
           E+F SD+S
Sbjct: 586 EIFISDSS 593



>XP_005251676
          Length = 1089

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   VFFSDTSV 9
           VFFSD+ V
Sbjct: 559 VFFSDSFV 566



>XP_005271958
          Length = 941

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   EVFFSDTS 8
           E+F SD+S
Sbjct: 567 EIFISDSS 574



>NP_064592
          Length = 1561

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    EVFFSD 6
            EVFF+D
Sbjct: 1359 EVFFND 1364



>NP_777596
          Length = 692

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   EVFFSDTSV 9
           EV+  DTS+
Sbjct: 181 EVYLLDTSI 189



>NP_001121361
          Length = 308

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   FSDTSV 9
           FSDTS+
Sbjct: 137 FSDTSI 142



>NP_057714
          Length = 308

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   FSDTSV 9
           FSDTS+
Sbjct: 137 FSDTSI 142



>NP_001121362
          Length = 281

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   FSDTSV 9
           FSDTS+
Sbjct: 110 FSDTSI 115



>NP_001239049
          Length = 1323

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   EVFFSD 6
           E+FFSD
Sbjct: 745 ELFFSD 750



>NP_055963
          Length = 1323

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   EVFFSD 6
           E+FFSD
Sbjct: 745 ELFFSD 750



>NP_001165422
          Length = 234

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  FFSDTSV 9
          FF+D+SV
Sbjct: 15 FFADSSV 21



>NP_001239053
          Length = 1143

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   EVFFSD 6
           E+FFSD
Sbjct: 745 ELFFSD 750



>NP_001239051
          Length = 1288

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   EVFFSD 6
           E+FFSD
Sbjct: 710 ELFFSD 715



>NP_001239048
          Length = 1330

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   EVFFSD 6
           E+FFSD
Sbjct: 745 ELFFSD 750



>NP_001008271
          Length = 235

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  FFSDTSV 9
          FF+D+SV
Sbjct: 15 FFADSSV 21



>XP_005254494
          Length = 308

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   FSDTSV 9
           FSDTS+
Sbjct: 137 FSDTSI 142



>XP_005254493
          Length = 308

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   FSDTSV 9
           FSDTS+
Sbjct: 137 FSDTSI 142



>XP_006720627
          Length = 308

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   FSDTSV 9
           FSDTS+
Sbjct: 137 FSDTSI 142



>XP_005252384
          Length = 841

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   EVFFSD 6
           EVFF+D
Sbjct: 639 EVFFND 644



>XP_005250004
          Length = 265

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  FFSDTSV 9
          FF+D+SV
Sbjct: 15 FFADSSV 21



>XP_005250003
          Length = 265

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  FFSDTSV 9
          FF+D+SV
Sbjct: 15 FFADSSV 21



>XP_005247048
          Length = 1107

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   EVFFSD 6
           E+FFSD
Sbjct: 529 ELFFSD 534



>XP_005247047
          Length = 1137

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   EVFFSD 6
           E+FFSD
Sbjct: 559 ELFFSD 564



>NP_002144
          Length = 387

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3 FFSDTS 8
         FFSDT+
Sbjct: 4 FFSDTA 9



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.322    0.132    0.349 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 472,303
Number of extensions: 719
Number of successful extensions: 37
Number of sequences better than 10000.0: 37
Number of HSP's gapped: 37
Number of HSP's successfully gapped: 37
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)