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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005091                                                              19     665
NP_653080                                                              19     665
XP_005267292                                                           19     665
XP_005267291                                                           19     665
XP_005267290                                                           19     665
NP_060876                                                              16    7355
NP_008952                                                              15    9605
NP_055523                                                              15    9605
XP_005259634                                                           15    9605
XP_006719769                                                           15    9605
NP_060401                                                              15    9605

>NP_005091
          Length = 1782

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ETTTEMLTS 9
            ET TEMLTS
Sbjct: 1566 ETATEMLTS 1574



>NP_653080
          Length = 1684

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ETTTEMLTS 9
            ET TEMLTS
Sbjct: 1468 ETATEMLTS 1476



>XP_005267292
          Length = 1677

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ETTTEMLTS 9
            ET TEMLTS
Sbjct: 1461 ETATEMLTS 1469



>XP_005267291
          Length = 1684

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ETTTEMLTS 9
            ET TEMLTS
Sbjct: 1468 ETATEMLTS 1476



>XP_005267290
          Length = 1782

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ETTTEMLTS 9
            ET TEMLTS
Sbjct: 1566 ETATEMLTS 1574



>NP_060876
          Length = 5412

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  ETTTEMLTS 9
          +T T+M+TS
Sbjct: 91 DTLTQMMTS 99



>NP_008952
          Length = 212

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  ETTTEML 7
          ETT EML
Sbjct: 17 ETTEEML 23



>NP_055523
          Length = 2209

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   ETTTEML 7
           ETT EML
Sbjct: 484 ETTQEML 490



>XP_005259634
          Length = 248

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   TTTEMLTS 9
           +TT MLTS
Sbjct: 232 STTAMLTS 239



>XP_006719769
          Length = 2209

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   ETTTEML 7
           ETT EML
Sbjct: 484 ETTQEML 490



>NP_060401
          Length = 469

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ETTTEMLTS 9
           +TT E+LT+
Sbjct: 347 QTTQELLTT 355



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.285   0.0965    0.214 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 349,304
Number of extensions: 497
Number of successful extensions: 11
Number of sequences better than 10000.0: 11
Number of HSP's gapped: 11
Number of HSP's successfully gapped: 11
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (14.9 bits)
S2: 28 (15.4 bits)