Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_443179                                                              22     134
NP_001139320                                                           18    1935
NP_001139321                                                           18    1935
NP_001139323                                                           18    1935
NP_006008                                                              18    1935
NP_001139322                                                           18    1935
NP_001139324                                                           18    1935
NP_001139319                                                           18    1935
XP_005248253                                                           18    1935
XP_005248252                                                           18    1935
XP_006714036                                                           18    1935
NP_002998                                                              17    2528
NP_002999                                                              17    2528
NP_079279                                                              16    7355
NP_001165599                                                           16    7355
NP_757366                                                              16    7355
XP_005254400                                                           16    7355
XP_005254399                                                           16    7355
XP_006719245                                                           16    7355
XP_006719244                                                           16    7355
NP_005471                                                              16    7355
NP_001080                                                              15    9605
NP_059111                                                              15    9605
NP_001244123                                                           15    9605
NP_001244124                                                           15    9605
NP_036549                                                              15    9605
NP_858057                                                              15    9605
NP_005291                                                              15    9605
XP_005272654                                                           15    9605
XP_005257273                                                           15    9605
XP_005269023                                                           15    9605
XP_005269022                                                           15    9605
XP_005269021                                                           15    9605
XP_006719516                                                           15    9605
XP_005269019                                                           15    9605
XP_006719515                                                           15    9605
NP_057572                                                              15    9605
NP_001137260                                                           15    9605
NP_001137261                                                           15    9605
NP_001137259                                                           15    9605
NP_958847                                                              15    9605
NP_001091048                                                           15    9605
NP_958923                                                              15    9605
NP_958846                                                              15    9605
NP_958848                                                              15    9605
>NP_443179
          Length = 2170

 Score = 21.6 bits (44), Expect =   134
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   ETRDSHQAK 9
           ETRDSHQA+
Sbjct: 468 ETRDSHQAR 476
>NP_001139320
          Length = 856

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  ETRDSHQA 8
          ET+DSH+A
Sbjct: 42 ETQDSHKA 49
>NP_001139321
          Length = 842

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  ETRDSHQA 8
          ET+DSH+A
Sbjct: 42 ETQDSHKA 49
>NP_001139323
          Length = 833

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  ETRDSHQA 8
          ET+DSH+A
Sbjct: 42 ETQDSHKA 49
>NP_006008
          Length = 865

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  ETRDSHQA 8
          ET+DSH+A
Sbjct: 42 ETQDSHKA 49
>NP_001139322
          Length = 834

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  ETRDSHQA 8
          ET+DSH+A
Sbjct: 42 ETQDSHKA 49
>NP_001139324
          Length = 825

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  ETRDSHQA 8
          ET+DSH+A
Sbjct: 42 ETQDSHKA 49
>NP_001139319
          Length = 856

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  ETRDSHQA 8
          ET+DSH+A
Sbjct: 42 ETQDSHKA 49
>XP_005248253
          Length = 825

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  ETRDSHQA 8
          ET+DSH+A
Sbjct: 42 ETQDSHKA 49
>XP_005248252
          Length = 833

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  ETRDSHQA 8
          ET+DSH+A
Sbjct: 42 ETQDSHKA 49
>XP_006714036
          Length = 856

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  ETRDSHQA 8
          ET+DSH+A
Sbjct: 42 ETQDSHKA 49
>NP_002998
          Length = 462

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ETRDSHQAK 9
           ET++SHQ K
Sbjct: 209 ETKNSHQNK 217
>NP_002999
          Length = 582

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ETRDSHQAK 9
           ET++SHQ K
Sbjct: 209 ETKNSHQNK 217
>NP_079279
          Length = 1910

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1    ETRDSHQ 7
            E +DSHQ
Sbjct: 1548 ERKDSHQ 1554
>NP_001165599
          Length = 1331

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   ETRDSH 6
           E+RDSH
Sbjct: 571 ESRDSH 576
>NP_757366
          Length = 1332

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   ETRDSH 6
           E+RDSH
Sbjct: 571 ESRDSH 576
>XP_005254400
          Length = 1378

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   ETRDSH 6
           E+RDSH
Sbjct: 618 ESRDSH 623
>XP_005254399
          Length = 1379

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   ETRDSH 6
           E+RDSH
Sbjct: 618 ESRDSH 623
>XP_006719245
          Length = 776

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   TRDSHQA 8
           TRDSH +
Sbjct: 296 TRDSHDS 302
>XP_006719244
          Length = 868

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   TRDSHQA 8
           TRDSH +
Sbjct: 296 TRDSHDS 302
>NP_005471
          Length = 778

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   TRDSHQA 8
           TRDSH +
Sbjct: 296 TRDSHDS 302
>NP_001080
          Length = 1704

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 2    TRDSHQA 8
            TRD H A
Sbjct: 1173 TRDGHMA 1179
>NP_059111
          Length = 587

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   ETRDSHQAK 9
           ETR  H AK
Sbjct: 226 ETRLEHMAK 234
>NP_001244123
          Length = 555

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   ETRDSHQAK 9
           ETR  H AK
Sbjct: 194 ETRLEHMAK 202
>NP_001244124
          Length = 505

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   ETRDSHQAK 9
           ETR  H AK
Sbjct: 144 ETRLEHMAK 152
>NP_036549
          Length = 332

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 3   RDSHQ 7
           RDSHQ
Sbjct: 174 RDSHQ 178
>NP_858057
          Length = 268

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 3   RDSHQ 7
           RDSHQ
Sbjct: 174 RDSHQ 178
>NP_005291
          Length = 381

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 3   RDSHQAK 9
           RD H AK
Sbjct: 208 RDKHNAK 214
>XP_005272654
          Length = 381

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 3   RDSHQAK 9
           RD H AK
Sbjct: 208 RDKHNAK 214
>XP_005257273
          Length = 309

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 3   RDSHQ 7
           RDSHQ
Sbjct: 151 RDSHQ 155
>XP_005269023
          Length = 300

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 2  TRDSHQA 8
          TRD H A
Sbjct: 39 TRDDHSA 45
>XP_005269022
          Length = 340

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 2  TRDSHQA 8
          TRD H A
Sbjct: 32 TRDDHSA 38
>XP_005269021
          Length = 355

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 2   TRDSHQA 8
           TRD H A
Sbjct: 94  TRDDHSA 100
>XP_006719516
          Length = 366

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 2   TRDSHQA 8
           TRD H A
Sbjct: 94  TRDDHSA 100
>XP_005269019
          Length = 409

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 2  TRDSHQA 8
          TRD H A
Sbjct: 32 TRDDHSA 38
>XP_006719515
          Length = 423

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 2   TRDSHQA 8
           TRD H A
Sbjct: 94  TRDDHSA 100
>NP_057572
          Length = 458

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 2  TRDSHQA 8
          TRD H A
Sbjct: 87 TRDDHSA 93
>NP_001137260
          Length = 348

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 2  TRDSHQA 8
          TRD H A
Sbjct: 87 TRDDHSA 93
>NP_001137261
          Length = 293

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 2  TRDSHQA 8
          TRD H A
Sbjct: 32 TRDDHSA 38
>NP_001137259
          Length = 434

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 2  TRDSHQA 8
          TRD H A
Sbjct: 32 TRDDHSA 38
>NP_958847
          Length = 367

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 2  TRDSHQA 8
          TRD H A
Sbjct: 87 TRDDHSA 93
>NP_001091048
          Length = 381

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 3   RDSHQAK 9
           RD H AK
Sbjct: 208 RDKHNAK 214
>NP_958923
          Length = 374

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 2   TRDSHQA 8
           TRD H A
Sbjct: 94  TRDDHSA 100
>NP_958846
          Length = 258

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 2  TRDSHQA 8
          TRD H A
Sbjct: 39 TRDDHSA 45
>NP_958848
          Length = 319

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 2  TRDSHQA 8
          TRD H A
Sbjct: 39 TRDDHSA 45
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.298    0.107    0.280 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 653,137
Number of extensions: 813
Number of successful extensions: 45
Number of sequences better than 10000.0: 45
Number of HSP's gapped: 45
Number of HSP's successfully gapped: 45
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.2 bits)
S2: 28 (15.4 bits)

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