Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_064450                                                              21     229
XP_005273257                                                           21     229
NP_079046                                                              19     869
XP_006712828                                                           19     869
XP_006712827                                                           19     869
XP_006712826                                                           19     869
XP_006712825                                                           19     869
NP_612450                                                              18    1135
XP_006715390                                                           18    1135
NP_003117                                                              17    3301
NP_001269512                                                           16    5631
NP_001269514                                                           16    5631
NP_001269511                                                           16    5631
NP_001269513                                                           16    5631
NP_115724                                                              16    5631
NP_689609                                                              16    5631
NP_775808                                                              16    5631
XP_005277669                                                           16    5631
XP_005277638                                                           16    5631
XP_005277637                                                           16    5631
XP_005277636                                                           16    5631
XP_006713034                                                           16    5631
XP_006713033                                                           16    5631
XP_006713032                                                           16    5631
XP_005264924                                                           16    5631
XP_006710765                                                           16    5631
XP_005244815                                                           16    5631
NP_001003795                                                           16    5631
NP_036413                                                              16    7355
NP_067675                                                              16    7355
NP_079471                                                              16    7355
NP_060866                                                              16    7355
XP_006716559                                                           16    7355
XP_005250379                                                           16    7355
XP_006711828                                                           16    7355
XP_006711827                                                           16    7355
XP_006711826                                                           16    7355
NP_859076                                                              16    7355
NP_001001966                                                           16    7355
YP_003024028                                                           16    7355
NP_001136272                                                           15    9605
NP_055660                                                              15    9605
NP_060338                                                              15    9605
NP_006370                                                              15    9605
XP_005257947                                                           15    9605
XP_005271664                                                           15    9605
XP_006718929                                                           15    9605
XP_005271663                                                           15    9605
XP_005271662                                                           15    9605
XP_005271661                                                           15    9605
XP_005271660                                                           15    9605
XP_005250170                                                           15    9605
XP_005273189                                                           15    9605
XP_005273188                                                           15    9605
NP_001004734                                                           15    9605
>NP_064450
          Length = 1722

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ETRAYFFMK 9
            ET AYFFMK
Sbjct: 1640 ETTAYFFMK 1648
>XP_005273257
          Length = 1726

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ETRAYFFMK 9
            ET AYFFMK
Sbjct: 1644 ETTAYFFMK 1652
>NP_079046
          Length = 407

 Score = 18.9 bits (37), Expect =   869
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   TRAYFFMK 9
           TRAYFF K
Sbjct: 337 TRAYFFTK 344
>XP_006712828
          Length = 357

 Score = 18.9 bits (37), Expect =   869
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   TRAYFFMK 9
           TRAYFF K
Sbjct: 287 TRAYFFTK 294
>XP_006712827
          Length = 362

 Score = 18.9 bits (37), Expect =   869
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   TRAYFFMK 9
           TRAYFF K
Sbjct: 292 TRAYFFTK 299
>XP_006712826
          Length = 407

 Score = 18.9 bits (37), Expect =   869
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   TRAYFFMK 9
           TRAYFF K
Sbjct: 337 TRAYFFTK 344
>XP_006712825
          Length = 455

 Score = 18.9 bits (37), Expect =   869
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   TRAYFFMK 9
           TRAYFF K
Sbjct: 385 TRAYFFTK 392
>NP_612450
          Length = 522

 Score = 18.5 bits (36), Expect =  1135
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   TRAYFFMK 9
           TRAY+F+K
Sbjct: 245 TRAYYFVK 252
>XP_006715390
          Length = 445

 Score = 18.5 bits (36), Expect =  1135
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   TRAYFFMK 9
           TRAY+F+K
Sbjct: 245 TRAYYFVK 252
>NP_003117
          Length = 2419

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    ETRAYF 6
            ETRAYF
Sbjct: 2175 ETRAYF 2180
>NP_001269512
          Length = 433

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ETRAYFFMK 9
           E+RA+FF +
Sbjct: 324 ESRAHFFQQ 332
>NP_001269514
          Length = 450

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ETRAYFFMK 9
           E+RA+FF +
Sbjct: 333 ESRAHFFQQ 341
>NP_001269511
          Length = 442

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ETRAYFFMK 9
           E+RA+FF +
Sbjct: 333 ESRAHFFQQ 341
>NP_001269513
          Length = 341

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ETRAYFFMK 9
           E+RA+FF +
Sbjct: 232 ESRAHFFQQ 240
>NP_115724
          Length = 442

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ETRAYFFMK 9
           E+RA+FF +
Sbjct: 333 ESRAHFFQQ 341
>NP_689609
          Length = 284

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   TRAYFF 7
           TR+YFF
Sbjct: 217 TRSYFF 222
>NP_775808
          Length = 949

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   RAYFFMK 9
           RAYFF++
Sbjct: 434 RAYFFVE 440
>XP_005277669
          Length = 949

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   RAYFFMK 9
           RAYFF++
Sbjct: 434 RAYFFVE 440
>XP_005277638
          Length = 755

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   RAYFFMK 9
           RAYFF++
Sbjct: 240 RAYFFVE 246
>XP_005277637
          Length = 878

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   RAYFFMK 9
           RAYFF++
Sbjct: 363 RAYFFVE 369
>XP_005277636
          Length = 949

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   RAYFFMK 9
           RAYFF++
Sbjct: 434 RAYFFVE 440
>XP_006713034
          Length = 239

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   TRAYFF 7
           TR+YFF
Sbjct: 172 TRSYFF 177
>XP_006713033
          Length = 284

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   TRAYFF 7
           TR+YFF
Sbjct: 217 TRSYFF 222
>XP_006713032
          Length = 284

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   TRAYFF 7
           TR+YFF
Sbjct: 217 TRSYFF 222
>XP_005264924
          Length = 284

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   TRAYFF 7
           TR+YFF
Sbjct: 217 TRSYFF 222
>XP_006710765
          Length = 343

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ETRAYFFMK 9
           E+RA+FF +
Sbjct: 232 ESRAHFFQQ 240
>XP_005244815
          Length = 444

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ETRAYFFMK 9
           E+RA+FF +
Sbjct: 333 ESRAHFFQQ 341
>NP_001003795
          Length = 949

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   RAYFFMK 9
           RAYFF++
Sbjct: 434 RAYFFVE 440
>NP_036413
          Length = 630

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  ETRAYFF 7
          ET+ YFF
Sbjct: 79 ETQQYFF 85
>NP_067675
          Length = 343

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   ETRAYFFMK 9
           ET+AY F +
Sbjct: 123 ETKAYVFFE 131
>NP_079471
          Length = 372

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   ETRAYFFMK 9
           ET+AY F +
Sbjct: 153 ETKAYVFFE 161
>NP_060866
          Length = 378

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 2   TRAYFF 7
           TR YFF
Sbjct: 320 TRVYFF 325
>XP_006716559
          Length = 262

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  ETRAYFFMK 9
          ET+AY F +
Sbjct: 43 ETKAYVFFE 51
>XP_005250379
          Length = 372

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  ETRAYFF 7
          ET+ YFF
Sbjct: 79 ETQQYFF 85
>XP_006711828
          Length = 332

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2  TRAYFFMK 9
          T  YFF+K
Sbjct: 78 TPVYFFLK 85
>XP_006711827
          Length = 309

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2  TRAYFFMK 9
          T  YFF+K
Sbjct: 55 TPVYFFLK 62
>XP_006711826
          Length = 332

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2  TRAYFFMK 9
          T  YFF+K
Sbjct: 78 TPVYFFLK 85
>NP_859076
          Length = 378

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 2   TRAYFF 7
           TR YFF
Sbjct: 320 TRVYFF 325
>NP_001001966
          Length = 309

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2  TRAYFFMK 9
          T  YFF+K
Sbjct: 55 TPVYFFLK 62
>YP_003024028
          Length = 513

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   ETRAYF 6
           +TRAYF
Sbjct: 300 DTRAYF 305
>NP_001136272
          Length = 3144

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 3   RAYFFMK 9
           R YFF K
Sbjct: 662 RGYFFRK 668
>NP_055660
          Length = 907

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  ETRAYFFM 8
          ETRA +F+
Sbjct: 19 ETRARYFL 26
>NP_060338
          Length = 705

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   TRAYFFM 8
           T+AYF M
Sbjct: 174 TKAYFHM 180
>NP_006370
          Length = 751

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 6/9 (66%)

Query: 1   ETRAYFFMK 9
           + + YFF K
Sbjct: 237 DAKVYFFFK 245
>XP_005257947
          Length = 1935

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   ETRAYF 6
           ETR YF
Sbjct: 717 ETRVYF 722
>XP_005271664
          Length = 732

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   TRAYFFM 8
           T+AYF M
Sbjct: 174 TKAYFHM 180
>XP_006718929
          Length = 756

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   TRAYFFM 8
           T+AYF M
Sbjct: 174 TKAYFHM 180
>XP_005271663
          Length = 774

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   TRAYFFM 8
           T+AYF M
Sbjct: 174 TKAYFHM 180
>XP_005271662
          Length = 777

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   TRAYFFM 8
           T+AYF M
Sbjct: 174 TKAYFHM 180
>XP_005271661
          Length = 777

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   TRAYFFM 8
           T+AYF M
Sbjct: 174 TKAYFHM 180
>XP_005271660
          Length = 777

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   TRAYFFM 8
           T+AYF M
Sbjct: 174 TKAYFHM 180
>XP_005250170
          Length = 693

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 6/9 (66%)

Query: 1   ETRAYFFMK 9
           + + YFF K
Sbjct: 179 DAKVYFFFK 187
>XP_005273189
          Length = 311

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2  TRAYFFMK 9
          T  YFF+K
Sbjct: 55 TPMYFFLK 62
>XP_005273188
          Length = 328

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2  TRAYFFMK 9
          T  YFF+K
Sbjct: 72 TPMYFFLK 79
>NP_001004734
          Length = 311

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2  TRAYFFMK 9
          T  YFF+K
Sbjct: 55 TPMYFFLK 62
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.342    0.142    0.429 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 439,360
Number of extensions: 472
Number of successful extensions: 55
Number of sequences better than 10000.0: 55
Number of HSP's gapped: 55
Number of HSP's successfully gapped: 55
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.6 bits)
S2: 28 (15.4 bits)

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