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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001120972                                                           18    1135
XP_006716051                                                           18    1135
NP_060141                                                              17    3301
NP_932765                                                              16    5631
NP_056437                                                              16    5631
NP_001743                                                              16    7355
NP_775809                                                              16    7355
XP_006716575                                                           16    7355
NP_001013425                                                           15    9605
NP_056473                                                              15    9605
NP_006353                                                              15    9605
XP_006718483                                                           15    9605
XP_006718482                                                           15    9605
XP_006718481                                                           15    9605
NP_001010889                                                           15    9605

>NP_001120972
          Length = 1408

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ESLNIVSFL 9
           E LNIVSFL
Sbjct: 760 EPLNIVSFL 768



>XP_006716051
          Length = 1427

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ESLNIVSFL 9
           E LNIVSFL
Sbjct: 779 EPLNIVSFL 787



>NP_060141
          Length = 677

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   ESLNIVSFL 9
           E LNI+SFL
Sbjct: 485 EVLNILSFL 493



>NP_932765
          Length = 482

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   ESLNIVSF 8
           ESLNIV +
Sbjct: 215 ESLNIVKY 222



>NP_056437
          Length = 482

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   ESLNIVSF 8
           ESLNIV +
Sbjct: 215 ESLNIVKY 222



>NP_001743
          Length = 527

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   ESLNIVSFL 9
           ESL+ VSFL
Sbjct: 191 ESLHQVSFL 199



>NP_775809
          Length = 436

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ESLNIVSFL 9
           ES NI+SF+
Sbjct: 351 ESGNIISFV 359



>XP_006716575
          Length = 515

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ESLNIVSFL 9
           ES NI+SF+
Sbjct: 351 ESGNIISFV 359



>NP_001013425
          Length = 476

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SLNIVSFL 9
           S+N VSFL
Sbjct: 274 SMNSVSFL 281



>NP_056473
          Length = 749

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ESLNIVSFL 9
           ESL +++FL
Sbjct: 307 ESLRVLAFL 315



>NP_006353
          Length = 619

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   LNIVSFL 9
           LN+V+FL
Sbjct: 460 LNVVAFL 466



>XP_006718483
          Length = 605

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   LNIVSFL 9
           LN+V+FL
Sbjct: 460 LNVVAFL 466



>XP_006718482
          Length = 619

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   LNIVSFL 9
           LN+V+FL
Sbjct: 460 LNVVAFL 466



>XP_006718481
          Length = 633

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   LNIVSFL 9
           LN+V+FL
Sbjct: 460 LNVVAFL 466



>NP_001010889
          Length = 476

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SLNIVSFL 9
           S+N VSFL
Sbjct: 274 SMNSVSFL 281



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.322    0.139    0.337 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 352,656
Number of extensions: 470
Number of successful extensions: 15
Number of sequences better than 10000.0: 15
Number of HSP's gapped: 15
Number of HSP's successfully gapped: 15
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)