Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001268421                                                           19     869
NP_000170                                                              19     869
NP_001268422                                                           19     869
NP_001268423                                                           19     869
XP_005264328                                                           19     869
NP_001371                                                              16    7355
NP_001277152                                                           16    7355
NP_001277131                                                           16    7355
NP_849191                                                              16    7355
NP_001092105                                                           16    7355
NP_998771                                                              16    7355
NP_056392                                                              16    7355
NP_001138997                                                           16    7355
NP_001138995                                                           16    7355
NP_001138994                                                           16    7355
NP_001138993                                                           16    7355
NP_001138992                                                           16    7355
XP_006714532                                                           16    7355
NP_115511                                                              16    7355
NP_001136277                                                           15    9605
NP_005620                                                              15    9605
NP_055043                                                              15    9605
NP_001269665                                                           15    9605
NP_001020016                                                           15    9605
NP_001139807                                                           15    9605
NP_060527                                                              15    9605
NP_116201                                                              15    9605
NP_877499                                                              15    9605
NP_964012                                                              15    9605
NP_008865                                                              15    9605
NP_001248309                                                           15    9605
XP_006713311                                                           15    9605
XP_006713310                                                           15    9605
XP_005264819                                                           15    9605
XP_006713309                                                           15    9605
XP_006710903                                                           15    9605
XP_006710057                                                           15    9605
NP_001139669                                                           15    9605
>NP_001268421
          Length = 1230

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ESIFFVELS 9
            ES FFVELS
Sbjct: 1057 ESTFFVELS 1065
>NP_000170
          Length = 1360

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ESIFFVELS 9
            ES FFVELS
Sbjct: 1187 ESTFFVELS 1195
>NP_001268422
          Length = 1058

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ESIFFVELS 9
           ES FFVELS
Sbjct: 885 ESTFFVELS 893
>NP_001268423
          Length = 1058

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ESIFFVELS 9
           ES FFVELS
Sbjct: 885 ESTFFVELS 893
>XP_005264328
          Length = 1261

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ESIFFVELS 9
            ES FFVELS
Sbjct: 1088 ESTFFVELS 1096
>NP_001371
          Length = 1865

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    ESIFFVELS 9
            +S+F VE+S
Sbjct: 1480 KSVFMVEIS 1488
>NP_001277152
          Length = 1886

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    ESIFFVELS 9
            +S+F VE+S
Sbjct: 1501 KSVFMVEIS 1509
>NP_001277131
          Length = 869

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   FFVELS 9
           FFVELS
Sbjct: 380 FFVELS 385
>NP_849191
          Length = 994

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   FFVELS 9
           FFVELS
Sbjct: 505 FFVELS 510
>NP_001092105
          Length = 993

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   FFVELS 9
           FFVELS
Sbjct: 505 FFVELS 510
>NP_998771
          Length = 516

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  SIFFVE 7
          SIFFVE
Sbjct: 70 SIFFVE 75
>NP_056392
          Length = 980

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   ESIFFVE 7
           ES+FF E
Sbjct: 303 ESVFFAE 309
>NP_001138997
          Length = 983

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   ESIFFVE 7
           ES+FF E
Sbjct: 306 ESVFFAE 312
>NP_001138995
          Length = 972

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   ESIFFVE 7
           ES+FF E
Sbjct: 295 ESVFFAE 301
>NP_001138994
          Length = 951

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   ESIFFVE 7
           ES+FF E
Sbjct: 274 ESVFFAE 280
>NP_001138993
          Length = 980

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   ESIFFVE 7
           ES+FF E
Sbjct: 303 ESVFFAE 309
>NP_001138992
          Length = 980

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   ESIFFVE 7
           ES+FF E
Sbjct: 303 ESVFFAE 309
>XP_006714532
          Length = 980

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   ESIFFVE 7
           ES+FF E
Sbjct: 303 ESVFFAE 309
>NP_115511
          Length = 825

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   ESIFFVEL 8
           ES+F +EL
Sbjct: 811 ESVFHIEL 818
>NP_001136277
          Length = 625

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   IFFVELS 9
           IFF+E+S
Sbjct: 105 IFFLEIS 111
>NP_005620
          Length = 635

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   IFFVELS 9
           IFF+E+S
Sbjct: 105 IFFLEIS 111
>NP_055043
          Length = 636

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  IFFVELS 9
          +FF+ELS
Sbjct: 90 LFFLELS 96
>NP_001269665
          Length = 480

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   SIFFVE 7
           SIFF+E
Sbjct: 437 SIFFIE 442
>NP_001020016
          Length = 633

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  IFFVELS 9
          +FF+ELS
Sbjct: 80 LFFMELS 86
>NP_001139807
          Length = 623

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  IFFVELS 9
          +FF+ELS
Sbjct: 7  LFFLELS 13
>NP_060527
          Length = 289

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   IFFVELS 9
           +FF+ELS
Sbjct: 114 LFFLELS 120
>NP_116201
          Length = 529

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   SIFFVE 7
           SIFF+E
Sbjct: 486 SIFFIE 491
>NP_877499
          Length = 730

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   IFFVELS 9
           +FF+ELS
Sbjct: 114 LFFLELS 120
>NP_964012
          Length = 706

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   IFFVELS 9
           +FF+ELS
Sbjct: 153 LFFMELS 159
>NP_008865
          Length = 652

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   IFFVELS 9
           +FF+ELS
Sbjct: 99  LFFMELS 105
>NP_001248309
          Length = 637

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  IFFVELS 9
          +FF+ELS
Sbjct: 84 LFFMELS 90
>XP_006713311
          Length = 939

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SIFFVELS 9
           SIFF+E S
Sbjct: 185 SIFFLERS 192
>XP_006713310
          Length = 1038

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SIFFVELS 9
           SIFF+E S
Sbjct: 284 SIFFLERS 291
>XP_005264819
          Length = 1128

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SIFFVELS 9
           SIFF+E S
Sbjct: 397 SIFFLERS 404
>XP_006713309
          Length = 1151

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SIFFVELS 9
           SIFF+E S
Sbjct: 397 SIFFLERS 404
>XP_006710903
          Length = 649

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   IFFVELS 9
           +FF+ELS
Sbjct: 153 LFFMELS 159
>XP_006710057
          Length = 99

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  IFFVELS 9
          IFF+E+S
Sbjct: 47 IFFLEIS 53
>NP_001139669
          Length = 7081

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    ESIFFVEL 8
            ++IFF EL
Sbjct: 3001 QNIFFTEL 3008
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.328    0.144    0.361 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 320,139
Number of extensions: 247
Number of successful extensions: 38
Number of sequences better than 10000.0: 38
Number of HSP's gapped: 38
Number of HSP's successfully gapped: 38
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)

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