Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              19     665
NP_001075109                                                           19     665
NP_597677                                                              18    1935
NP_056199                                                              18    1935
XP_005248339                                                           18    1935
XP_005248337                                                           18    1935
XP_006714531                                                           18    1935
XP_006714530                                                           18    1935
NP_037523                                                              17    4312
XP_006714661                                                           17    4312
XP_006714660                                                           17    4312
NP_982272                                                              16    5631
NP_001177387                                                           16    5631
NP_001180465                                                           16    5631
NP_056100                                                              16    5631
NP_443066                                                              16    5631
XP_005266731                                                           16    5631
XP_006722494                                                           16    5631
NP_085126                                                              16    7355
NP_001136116                                                           16    7355
XP_006712841                                                           16    7355
XP_006712840                                                           16    7355
NP_001275929                                                           15    9605
NP_001275930                                                           15    9605
NP_705837                                                              15    9605
NP_055504                                                              15    9605
NP_001277192                                                           15    9605
XP_006724226                                                           15    9605
XP_006724225                                                           15    9605
XP_006712687                                                           15    9605
XP_006712686                                                           15    9605
XP_006712685                                                           15    9605
XP_006712684                                                           15    9605
XP_006712683                                                           15    9605
XP_006712682                                                           15    9605
XP_006712681                                                           15    9605
XP_005246730                                                           15    9605
XP_005246729                                                           15    9605
XP_006712680                                                           15    9605
XP_006712679                                                           15    9605
NP_113689                                                              15    9605
NP_061731                                                              15    9605
>NP_008835
          Length = 4128

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    ESIAMYDII 9
            ESIAM+DII
Sbjct: 1299 ESIAMHDII 1307
>NP_001075109
          Length = 4097

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    ESIAMYDII 9
            ESIAM+DII
Sbjct: 1299 ESIAMHDII 1307
>NP_597677
          Length = 2804

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   ESIAMYDII 9
           E  AMYDII
Sbjct: 340 EKAAMYDII 348
>NP_056199
          Length = 2697

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   ESIAMYDII 9
           E  AMYDII
Sbjct: 340 EKAAMYDII 348
>XP_005248339
          Length = 2584

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   ESIAMYDII 9
           E  AMYDII
Sbjct: 120 EKAAMYDII 128
>XP_005248337
          Length = 2698

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   ESIAMYDII 9
           E  AMYDII
Sbjct: 340 EKAAMYDII 348
>XP_006714531
          Length = 2738

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   ESIAMYDII 9
           E  AMYDII
Sbjct: 340 EKAAMYDII 348
>XP_006714530
          Length = 2755

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   ESIAMYDII 9
           E  AMYDII
Sbjct: 340 EKAAMYDII 348
>NP_037523
          Length = 866

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ESIAMYDII 9
           +S+A+YD I
Sbjct: 690 DSVALYDAI 698
>XP_006714661
          Length = 486

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ESIAMYDII 9
           +S+A+YD I
Sbjct: 310 DSVALYDAI 318
>XP_006714660
          Length = 774

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ESIAMYDII 9
           +S+A+YD I
Sbjct: 690 DSVALYDAI 698
>NP_982272
          Length = 2099

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   SIAMYDI 8
           SIA+YD+
Sbjct: 287 SIALYDV 293
>NP_001177387
          Length = 1999

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   SIAMYDI 8
           SIA+YD+
Sbjct: 219 SIALYDV 225
>NP_001180465
          Length = 2031

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   SIAMYDI 8
           SIA+YD+
Sbjct: 219 SIALYDV 225
>NP_056100
          Length = 1490

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    SIAMYDI 8
            S+A+YDI
Sbjct: 1376 SVALYDI 1382
>NP_443066
          Length = 1457

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    SIAMYDI 8
            S+A+YDI
Sbjct: 1343 SVALYDI 1349
>XP_005266731
          Length = 982

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   SIAMYDI 8
           S+A+YDI
Sbjct: 868 SVALYDI 874
>XP_006722494
          Length = 1490

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    SIAMYDI 8
            S+A+YDI
Sbjct: 1376 SVALYDI 1382
>NP_085126
          Length = 1671

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  ESIAMYDII 9
          ES  MYD++
Sbjct: 14 ESSRMYDVL 22
>NP_001136116
          Length = 1700

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  ESIAMYDII 9
          ES  MYD++
Sbjct: 14 ESSRMYDVL 22
>XP_006712841
          Length = 1696

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  ESIAMYDII 9
          ES  MYD++
Sbjct: 39 ESSRMYDVL 47
>XP_006712840
          Length = 1725

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  ESIAMYDII 9
          ES  MYD++
Sbjct: 39 ESSRMYDVL 47
>NP_001275929
          Length = 824

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   ESIAMYDI 8
           + +AMYD+
Sbjct: 261 DRLAMYDV 268
>NP_001275930
          Length = 802

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   ESIAMYDI 8
           + +AMYD+
Sbjct: 261 DRLAMYDV 268
>NP_705837
          Length = 811

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   ESIAMYDI 8
           + +AMYD+
Sbjct: 270 DRLAMYDV 277
>NP_055504
          Length = 2186

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   SIAMYDI 8
           S+A+YD+
Sbjct: 426 SVALYDL 432
>NP_001277192
          Length = 2180

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   SIAMYDI 8
           S+A+YD+
Sbjct: 420 SVALYDL 426
>XP_006724226
          Length = 802

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   ESIAMYDI 8
           + +AMYD+
Sbjct: 261 DRLAMYDV 268
>XP_006724225
          Length = 802

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   ESIAMYDI 8
           + +AMYD+
Sbjct: 261 DRLAMYDV 268
>XP_006712687
          Length = 2166

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   SIAMYDI 8
           S+A+YD+
Sbjct: 439 SVALYDL 445
>XP_006712686
          Length = 2173

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   SIAMYDI 8
           S+A+YD+
Sbjct: 439 SVALYDL 445
>XP_006712685
          Length = 2175

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   SIAMYDI 8
           S+A+YD+
Sbjct: 439 SVALYDL 445
>XP_006712684
          Length = 2189

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   SIAMYDI 8
           S+A+YD+
Sbjct: 420 SVALYDL 426
>XP_006712683
          Length = 2195

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   SIAMYDI 8
           S+A+YD+
Sbjct: 426 SVALYDL 432
>XP_006712682
          Length = 2199

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   SIAMYDI 8
           S+A+YD+
Sbjct: 439 SVALYDL 445
>XP_006712681
          Length = 2199

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   SIAMYDI 8
           S+A+YD+
Sbjct: 439 SVALYDL 445
>XP_005246730
          Length = 2199

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   SIAMYDI 8
           S+A+YD+
Sbjct: 439 SVALYDL 445
>XP_005246729
          Length = 2201

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   SIAMYDI 8
           S+A+YD+
Sbjct: 439 SVALYDL 445
>XP_006712680
          Length = 2206

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   SIAMYDI 8
           S+A+YD+
Sbjct: 439 SVALYDL 445
>XP_006712679
          Length = 2208

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   SIAMYDI 8
           S+A+YD+
Sbjct: 439 SVALYDL 445
>NP_113689
          Length = 816

 Score = 15.4 bits (28), Expect =  9605
 Identities = 3/9 (33%), Positives = 9/9 (100%)

Query: 1   ESIAMYDII 9
           ES+++Y+++
Sbjct: 415 ESVSVYELV 423
>NP_061731
          Length = 936

 Score = 15.4 bits (28), Expect =  9605
 Identities = 3/9 (33%), Positives = 9/9 (100%)

Query: 1   ESIAMYDII 9
           ES+++Y+++
Sbjct: 415 ESVSVYELV 423
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.327    0.142    0.362 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 364,792
Number of extensions: 425
Number of successful extensions: 42
Number of sequences better than 10000.0: 42
Number of HSP's gapped: 42
Number of HSP's successfully gapped: 42
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)

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