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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_722576                                                              20     390
XP_005262424                                                           20     390
NP_510880                                                              17    3301
NP_976063                                                              17    3301
NP_067047                                                              16    5631
XP_006717281                                                           16    5631
XP_006717280                                                           16    5631
XP_006717279                                                           16    5631
XP_006717278                                                           16    5631
XP_006717277                                                           16    5631
XP_006717276                                                           16    5631
XP_006717275                                                           16    5631
XP_006717274                                                           16    5631
XP_006717273                                                           16    5631
XP_006717272                                                           16    5631
NP_001070865                                                           16    7355
NP_892017                                                              16    7355
XP_006710008                                                           16    7355
NP_001186930                                                           15    9605
NP_077719                                                              15    9605
NP_689678                                                              15    9605
NP_149052                                                              15    9605
XP_005257989                                                           15    9605
XP_006721787                                                           15    9605
XP_005257987                                                           15    9605
XP_005257986                                                           15    9605
XP_005257985                                                           15    9605
XP_006721786                                                           15    9605
XP_005257984                                                           15    9605
XP_005270959                                                           15    9605
XP_005270958                                                           15    9605
XP_006710730                                                           15    9605
NP_112217                                                              15    9605

>NP_722576
          Length = 3080

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    ESAMATSTP 9
            ES+MATSTP
Sbjct: 1501 ESSMATSTP 1509



>XP_005262424
          Length = 2875

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    ESAMATSTP 9
            ES+MATSTP
Sbjct: 1296 ESSMATSTP 1304



>NP_510880
          Length = 2054

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   ESAMATSTP 9
           ESA  TSTP
Sbjct: 148 ESASETSTP 156



>NP_976063
          Length = 2039

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   ESAMATSTP 9
           ESA  TSTP
Sbjct: 148 ESASETSTP 156



>NP_067047
          Length = 2506

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   MATSTP 9
           MATSTP
Sbjct: 988 MATSTP 993



>XP_006717281
          Length = 1702

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   MATSTP 9
           MATSTP
Sbjct: 988 MATSTP 993



>XP_006717280
          Length = 2221

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   MATSTP 9
           MATSTP
Sbjct: 988 MATSTP 993



>XP_006717279
          Length = 2506

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   MATSTP 9
           MATSTP
Sbjct: 988 MATSTP 993



>XP_006717278
          Length = 2566

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   MATSTP 9
           MATSTP
Sbjct: 988 MATSTP 993



>XP_006717277
          Length = 2567

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   MATSTP 9
           MATSTP
Sbjct: 988 MATSTP 993



>XP_006717276
          Length = 2567

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   MATSTP 9
           MATSTP
Sbjct: 988 MATSTP 993



>XP_006717275
          Length = 2567

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   MATSTP 9
           MATSTP
Sbjct: 988 MATSTP 993



>XP_006717274
          Length = 2567

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   MATSTP 9
           MATSTP
Sbjct: 988 MATSTP 993



>XP_006717273
          Length = 2567

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   MATSTP 9
           MATSTP
Sbjct: 988 MATSTP 993



>XP_006717272
          Length = 2567

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   MATSTP 9
           MATSTP
Sbjct: 988 MATSTP 993



>NP_001070865
          Length = 571

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ESAMATSTP 9
           +S++ATS P
Sbjct: 479 DSSLATSAP 487



>NP_892017
          Length = 587

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ESAMATSTP 9
           +S++ATS P
Sbjct: 495 DSSLATSAP 503



>XP_006710008
          Length = 2014

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    SAMATSTP 9
            S+M+T+TP
Sbjct: 1551 SSMSTTTP 1558



>NP_001186930
          Length = 1235

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   ESAMATSTP 9
           E AMA S P
Sbjct: 418 ECAMANSNP 426



>NP_077719
          Length = 2471

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   ESAMATSTP 9
           E AMA S P
Sbjct: 418 ECAMANSNP 426



>NP_689678
          Length = 336

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SAMATSTP 9
           ++MAT TP
Sbjct: 174 TSMATGTP 181



>NP_149052
          Length = 813

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   ESAMATS 7
           ESAMAT+
Sbjct: 650 ESAMATA 656



>XP_005257989
          Length = 338

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SAMATSTP 9
           ++MAT TP
Sbjct: 176 TSMATGTP 183



>XP_006721787
          Length = 264

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SAMATSTP 9
           ++MAT TP
Sbjct: 102 TSMATGTP 109



>XP_005257987
          Length = 264

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SAMATSTP 9
           ++MAT TP
Sbjct: 102 TSMATGTP 109



>XP_005257986
          Length = 264

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SAMATSTP 9
           ++MAT TP
Sbjct: 102 TSMATGTP 109



>XP_005257985
          Length = 264

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SAMATSTP 9
           ++MAT TP
Sbjct: 102 TSMATGTP 109



>XP_006721786
          Length = 361

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SAMATSTP 9
           ++MAT TP
Sbjct: 199 TSMATGTP 206



>XP_005257984
          Length = 364

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SAMATSTP 9
           ++MAT TP
Sbjct: 202 TSMATGTP 209



>XP_005270959
          Length = 2432

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   ESAMATSTP 9
           E AMA S P
Sbjct: 379 ECAMANSNP 387



>XP_005270958
          Length = 2432

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   ESAMATSTP 9
           E AMA S P
Sbjct: 379 ECAMANSNP 387



>XP_006710730
          Length = 2432

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   ESAMATSTP 9
           E AMA S P
Sbjct: 379 ECAMANSNP 387



>NP_112217
          Length = 1594

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    AMATSTP 9
            +M+TSTP
Sbjct: 1040 SMSTSTP 1046



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.284   0.0912    0.218 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 325,204
Number of extensions: 348
Number of successful extensions: 33
Number of sequences better than 10000.0: 33
Number of HSP's gapped: 33
Number of HSP's successfully gapped: 33
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 18 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (14.9 bits)
S2: 28 (15.4 bits)