Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_006715                                                              20     390
NP_005913                                                              20     390
XP_005267046                                                           20     390
XP_005267045                                                           20     390
NP_057006                                                              17    4312
NP_001123237                                                           17    4312
XP_006724887                                                           17    4312
NP_689634                                                              16    5631
XP_006717017                                                           16    5631
NP_004068                                                              16    7355
NP_006178                                                              16    7355
XP_005253946                                                           16    7355
XP_005271224                                                           16    7355
NP_001138528                                                           15    9605
NP_065161                                                              15    9605
XP_006726390                                                           15    9605
XP_006726389                                                           15    9605
XP_006726388                                                           15    9605
XP_006726387                                                           15    9605
XP_005260056                                                           15    9605
XP_005260055                                                           15    9605
XP_005260054                                                           15    9605
XP_006711142                                                           15    9605
XP_006711141                                                           15    9605
XP_006711140                                                           15    9605
XP_006710131                                                           15    9605
XP_006710130                                                           15    9605
XP_006710129                                                           15    9605
XP_006710128                                                           15    9605
>NP_006715
          Length = 1558

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   ERASRALGF 9
           ERAS+ALGF
Sbjct: 810 ERASKALGF 818
>NP_005913
          Length = 1608

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   ERASRALGF 9
           ERAS+ALGF
Sbjct: 810 ERASKALGF 818
>XP_005267046
          Length = 1554

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   ERASRALGF 9
           ERAS+ALGF
Sbjct: 810 ERASKALGF 818
>XP_005267045
          Length = 1604

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   ERASRALGF 9
           ERAS+ALGF
Sbjct: 810 ERASKALGF 818
>NP_057006
          Length = 373

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ERASRALGF 9
           ERA +A+G+
Sbjct: 341 ERAKKAMGY 349
>NP_001123237
          Length = 373

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ERASRALGF 9
           ERA +A+G+
Sbjct: 341 ERAKKAMGY 349
>XP_006724887
          Length = 373

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ERASRALGF 9
           ERA +A+G+
Sbjct: 341 ERAKKAMGY 349
>NP_689634
          Length = 431

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   ERASRALGF 9
           E ASR LG+
Sbjct: 216 EHASRLLGY 224
>XP_006717017
          Length = 434

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   ERASRALGF 9
           E ASR LG+
Sbjct: 219 EHASRLLGY 227
>NP_004068
          Length = 466

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   SRALGF 9
           SRALGF
Sbjct: 439 SRALGF 444
>NP_006178
          Length = 1087

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  ERASRALG 8
          E+A RALG
Sbjct: 27 EKARRALG 34
>XP_005253946
          Length = 1089

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  ERASRALG 8
          E+A RALG
Sbjct: 27 EKARRALG 34
>XP_005271224
          Length = 102

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  ERASRALG 8
          ERA RA+G
Sbjct: 83 ERAMRAVG 90
>NP_001138528
          Length = 704

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ERASRALGF 9
           + ASRA+G+
Sbjct: 346 KEASRAVGY 354
>NP_065161
          Length = 706

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ERASRALGF 9
           + ASRA+G+
Sbjct: 348 KEASRAVGY 356
>XP_006726390
          Length = 1353

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    ASRALGF 9
            ASRA GF
Sbjct: 1191 ASRAAGF 1197
>XP_006726389
          Length = 1471

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    ASRALGF 9
            ASRA GF
Sbjct: 1309 ASRAAGF 1315
>XP_006726388
          Length = 1489

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    ASRALGF 9
            ASRA GF
Sbjct: 1327 ASRAAGF 1333
>XP_006726387
          Length = 1497

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    ASRALGF 9
            ASRA GF
Sbjct: 1375 ASRAAGF 1381
>XP_005260056
          Length = 709

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ERASRALGF 9
           + ASRA+G+
Sbjct: 346 KEASRAVGY 354
>XP_005260055
          Length = 711

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ERASRALGF 9
           + ASRA+G+
Sbjct: 348 KEASRAVGY 356
>XP_005260054
          Length = 711

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ERASRALGF 9
           + ASRA+G+
Sbjct: 348 KEASRAVGY 356
>XP_006711142
          Length = 1471

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    ASRALGF 9
            ASRA GF
Sbjct: 1309 ASRAAGF 1315
>XP_006711141
          Length = 1489

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    ASRALGF 9
            ASRA GF
Sbjct: 1327 ASRAAGF 1333
>XP_006711140
          Length = 1497

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    ASRALGF 9
            ASRA GF
Sbjct: 1375 ASRAAGF 1381
>XP_006710131
          Length = 1353

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    ASRALGF 9
            ASRA GF
Sbjct: 1191 ASRAAGF 1197
>XP_006710130
          Length = 1471

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    ASRALGF 9
            ASRA GF
Sbjct: 1309 ASRAAGF 1315
>XP_006710129
          Length = 1489

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    ASRALGF 9
            ASRA GF
Sbjct: 1327 ASRAAGF 1333
>XP_006710128
          Length = 1497

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    ASRALGF 9
            ASRA GF
Sbjct: 1375 ASRAAGF 1381
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.326    0.135    0.365 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 256,295
Number of extensions: 389
Number of successful extensions: 29
Number of sequences better than 10000.0: 29
Number of HSP's gapped: 29
Number of HSP's successfully gapped: 29
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)

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