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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_002430                                                              20     390
NP_056261                                                              17    2528
XP_006711822                                                           17    2528
XP_006711821                                                           17    2528
NP_005712                                                              16    5631
NP_001139248                                                           16    5631
NP_004108                                                              16    5631
NP_001139249                                                           16    5631
NP_001139247                                                           16    5631
NP_001278253                                                           16    7355
NP_005488                                                              16    7355
XP_006722053                                                           16    7355
XP_006722052                                                           16    7355
XP_005256978                                                           16    7355
XP_005256977                                                           16    7355
NP_001073852                                                           16    7355
NP_001035830                                                           15    9605
NP_570123                                                              15    9605
NP_006678                                                              15    9605
XP_006714595                                                           15    9605
XP_006714594                                                           15    9605

>NP_002430
          Length = 1137

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   EQTAKVGDK 9
           EQ AKVGDK
Sbjct: 691 EQAAKVGDK 699



>NP_056261
          Length = 2275

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    EQTAKVGDK 9
            EQT K GDK
Sbjct: 1362 EQTEKDGDK 1370



>XP_006711822
          Length = 2266

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    EQTAKVGDK 9
            EQT K GDK
Sbjct: 1353 EQTEKDGDK 1361



>XP_006711821
          Length = 2301

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    EQTAKVGDK 9
            EQT K GDK
Sbjct: 1388 EQTEKDGDK 1396



>NP_005712
          Length = 418

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1  EQTAKVGDK 9
          +++AKVGD+
Sbjct: 38 KESAKVGDQ 46



>NP_001139248
          Length = 457

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  EQTAKVGDK 9
          E+T  +GDK
Sbjct: 44 EETPMIGDK 52



>NP_004108
          Length = 457

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  EQTAKVGDK 9
          E+T  +GDK
Sbjct: 44 EETPMIGDK 52



>NP_001139249
          Length = 268

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  EQTAKVGDK 9
          E+T  +GDK
Sbjct: 44 EETPMIGDK 52



>NP_001139247
          Length = 457

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  EQTAKVGDK 9
          E+T  +GDK
Sbjct: 44 EETPMIGDK 52



>NP_001278253
          Length = 2639

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    EQTAKVGD 8
            E+T +VGD
Sbjct: 2512 EETLRVGD 2519



>NP_005488
          Length = 396

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EQTAKVGDK 9
           E+ AKVG K
Sbjct: 367 EKKAKVGSK 375



>XP_006722053
          Length = 2608

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    EQTAKVGD 8
            E+T +VGD
Sbjct: 2481 EETLRVGD 2488



>XP_006722052
          Length = 2631

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    EQTAKVGD 8
            E+T +VGD
Sbjct: 2504 EETLRVGD 2511



>XP_005256978
          Length = 396

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EQTAKVGDK 9
           E+ AKVG K
Sbjct: 367 EKKAKVGSK 375



>XP_005256977
          Length = 396

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EQTAKVGDK 9
           E+ AKVG K
Sbjct: 367 EKKAKVGSK 375



>NP_001073852
          Length = 396

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EQTAKVGDK 9
           E+ AKVG K
Sbjct: 367 EKKAKVGSK 375



>NP_001035830
          Length = 321

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   EQTAKVGDK 9
           E+T  VGD+
Sbjct: 253 ERTVMVGDR 261



>NP_570123
          Length = 555

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  EQTAKVG 7
          E+TA+VG
Sbjct: 62 EETARVG 68



>NP_006678
          Length = 435

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   QTAKVGD 8
           +TAK GD
Sbjct: 105 ETAKTGD 111



>XP_006714595
          Length = 378

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  EQTAKVG 7
          E+TA+VG
Sbjct: 62 EETARVG 68



>XP_006714594
          Length = 527

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  EQTAKVG 7
          E+TA+VG
Sbjct: 62 EETARVG 68



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.293    0.116    0.288 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 438,400
Number of extensions: 490
Number of successful extensions: 21
Number of sequences better than 10000.0: 21
Number of HSP's gapped: 21
Number of HSP's successfully gapped: 21
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.0 bits)
S2: 28 (15.4 bits)