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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_055365                                                              18    1135
NP_036558                                                              18    1482
NP_001413                                                              18    1935
NP_938195                                                              18    1935
NP_001186623                                                           17    2528
NP_001186622                                                           17    2528
NP_001186621                                                           17    2528
NP_003031                                                              17    2528
NP_006222                                                              16    7355
NP_001278253                                                           16    7355
NP_005188                                                              16    7355
XP_006722053                                                           16    7355
XP_006722052                                                           16    7355
NP_001078940                                                           16    7355

>NP_055365
          Length = 1032

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   EQMVEAAGL 9
           EQ VEAAGL
Sbjct: 649 EQRVEAAGL 657



>NP_036558
          Length = 1217

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 1   EQMVEAAG 8
           E+MVEAAG
Sbjct: 821 EEMVEAAG 828



>NP_001413
          Length = 255

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   EQMVEAAGL 9
           E+ VEAAGL
Sbjct: 95  EEFVEAAGL 103



>NP_938195
          Length = 265

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   EQMVEAAGL 9
           E+ VEAAGL
Sbjct: 105 EEFVEAAGL 113



>NP_001186623
          Length = 1227

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   QMVEAAG 8
           QMVEAAG
Sbjct: 648 QMVEAAG 654



>NP_001186622
          Length = 1232

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   QMVEAAG 8
           QMVEAAG
Sbjct: 653 QMVEAAG 659



>NP_001186621
          Length = 1241

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   QMVEAAG 8
           QMVEAAG
Sbjct: 662 QMVEAAG 668



>NP_003031
          Length = 1241

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   QMVEAAG 8
           QMVEAAG
Sbjct: 662 QMVEAAG 668



>NP_006222
          Length = 2286

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    EQMVEAAGL 9
            +QMV+ AGL
Sbjct: 1066 DQMVKDAGL 1074



>NP_001278253
          Length = 2639

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    MVEAAGL 9
            +VEAAGL
Sbjct: 1379 LVEAAGL 1385



>NP_005188
          Length = 468

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   EQMVEAAG 8
           E+M EAAG
Sbjct: 429 EEMKEAAG 436



>XP_006722053
          Length = 2608

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    MVEAAGL 9
            +VEAAGL
Sbjct: 1348 LVEAAGL 1354



>XP_006722052
          Length = 2631

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    MVEAAGL 9
            +VEAAGL
Sbjct: 1379 LVEAAGL 1385



>NP_001078940
          Length = 490

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   EQMVEAAG 8
           E+M EAAG
Sbjct: 451 EEMKEAAG 458



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.307    0.122    0.292 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 307,018
Number of extensions: 523
Number of successful extensions: 14
Number of sequences better than 10000.0: 14
Number of HSP's gapped: 14
Number of HSP's successfully gapped: 14
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.4 bits)
S2: 28 (15.4 bits)