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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004251                                                              18    1135
NP_001003845                                                           17    4312
NP_079335                                                              17    4312
XP_006715631                                                           17    4312
XP_005246599                                                           17    4312
NP_006436                                                              16    5631
NP_001135886                                                           16    5631
NP_055175                                                              16    5631
NP_001035738                                                           16    5631
NP_001035737                                                           16    5631
NP_001230131                                                           16    5631
NP_001254505                                                           16    5631
NP_694942                                                              16    5631
NP_036548                                                              16    5631
NP_071403                                                              16    5631
NP_055293                                                              16    5631
NP_783165                                                              16    7355
XP_005268276                                                           16    7355
NP_004265                                                              16    7355
NP_004143                                                              15    9605

>NP_004251
          Length = 1208

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   EQIAALLRT 9
           E+IAALLRT
Sbjct: 714 ERIAALLRT 722



>NP_001003845
          Length = 398

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   QIAALLRT 9
           QIAALL+T
Sbjct: 253 QIAALLQT 260



>NP_079335
          Length = 715

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 1   EQIAALLR 8
           E+IAALLR
Sbjct: 328 EKIAALLR 335



>XP_006715631
          Length = 648

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 1   EQIAALLR 8
           E+IAALLR
Sbjct: 328 EKIAALLR 335



>XP_005246599
          Length = 442

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   QIAALLRT 9
           QIAALL+T
Sbjct: 297 QIAALLQT 304



>NP_006436
          Length = 2335

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1    EQIAALLRT 9
            E++AAL+R+
Sbjct: 1843 EEVAALIRS 1851



>NP_001135886
          Length = 141

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   EQIAALLRT 9
           EQI ALL T
Sbjct: 104 EQIEALLHT 112



>NP_055175
          Length = 239

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   EQIAALLR 8
           EQ+A+LLR
Sbjct: 115 EQLASLLR 122



>NP_001035738
          Length = 71

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1  EQIAALLRT 9
          EQI ALL T
Sbjct: 34 EQIEALLHT 42



>NP_001035737
          Length = 115

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1  EQIAALLRT 9
          EQI ALL T
Sbjct: 78 EQIEALLHT 86



>NP_001230131
          Length = 753

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   IAALLRT 9
           IAALLRT
Sbjct: 99  IAALLRT 105



>NP_001254505
          Length = 1118

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   EQIAALLR 8
           EQ+AAL+R
Sbjct: 145 EQLAALVR 152



>NP_694942
          Length = 1123

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   EQIAALLR 8
           EQ+AAL+R
Sbjct: 145 EQLAALVR 152



>NP_036548
          Length = 1105

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   IAALLRT 9
           IAALLRT
Sbjct: 451 IAALLRT 457



>NP_071403
          Length = 517

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3  IAALLRT 9
          IAALLRT
Sbjct: 74 IAALLRT 80



>NP_055293
          Length = 148

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   EQIAALLRT 9
           EQI ALL T
Sbjct: 111 EQIEALLHT 119



>NP_783165
          Length = 576

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  EQIAALL 7
          EQ+AALL
Sbjct: 14 EQVAALL 20



>XP_005268276
          Length = 2320

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   EQIAALLRT 9
           EQI ALL T
Sbjct: 119 EQIHALLET 127



>NP_004265
          Length = 2319

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   EQIAALLRT 9
           EQI ALL T
Sbjct: 119 EQIHALLET 127



>NP_004143
          Length = 228

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   EQIAALLRT 9
           E  AALLRT
Sbjct: 181 EDRAALLRT 189



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.323    0.131    0.305 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 290,701
Number of extensions: 464
Number of successful extensions: 20
Number of sequences better than 10000.0: 20
Number of HSP's gapped: 20
Number of HSP's successfully gapped: 20
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)