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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_055365                                                              20     299
NP_060626                                                              18    1135
NP_056193                                                              18    1135
XP_005271970                                                           17    3301
NP_434701                                                              17    4312
NP_434700                                                              17    4312
NP_001025006                                                           16    7355
NP_113631                                                              16    7355
NP_001161407                                                           16    7355
NP_001161406                                                           16    7355
NP_997698                                                              16    7355
NP_001597                                                              16    7355
XP_006717059                                                           16    7355
XP_006715849                                                           16    7355
XP_006715848                                                           16    7355
NP_001006658                                                           15    9605
NP_065830                                                              15    9605
NP_001107604                                                           15    9605
XP_005253558                                                           15    9605
XP_006719219                                                           15    9605
XP_005253556                                                           15    9605
XP_005253555                                                           15    9605
XP_005253210                                                           15    9605
XP_006712765                                                           15    9605
XP_006712764                                                           15    9605
XP_005246844                                                           15    9605
XP_006712763                                                           15    9605
XP_006712762                                                           15    9605
XP_006712761                                                           15    9605
NP_110410                                                              15    9605

>NP_055365
          Length = 1032

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ENYMITMRL 9
           ENYMI MRL
Sbjct: 205 ENYMIAMRL 213



>NP_060626
          Length = 4363

 Score = 18.5 bits (36), Expect =  1135
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    ENYMITMRL 9
            +NYM+ MRL
Sbjct: 3221 QNYMVRMRL 3229



>NP_056193
          Length = 4388

 Score = 18.5 bits (36), Expect =  1135
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    ENYMITMRL 9
            +NYM+ MRL
Sbjct: 3246 QNYMVRMRL 3254



>XP_005271970
          Length = 2931

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2    NYMITMR 8
            NYMIT R
Sbjct: 2906 NYMITFR 2912



>NP_434701
          Length = 492

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   ENYMITMRL 9
           ENY + MRL
Sbjct: 181 ENYDLAMRL 189



>NP_434700
          Length = 536

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   ENYMITMRL 9
           ENY + MRL
Sbjct: 181 ENYDLAMRL 189



>NP_001025006
          Length = 465

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   ENYMITMR 8
           ++YM+T+R
Sbjct: 321 QDYMLTLR 328



>NP_113631
          Length = 444

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   ENYMITMR 8
           ++YM+T+R
Sbjct: 300 QDYMLTLR 307



>NP_001161407
          Length = 353

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   ENYMITMR 8
           ++YM+T+R
Sbjct: 209 QDYMLTLR 216



>NP_001161406
          Length = 386

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   ENYMITMR 8
           ++YM+T+R
Sbjct: 242 QDYMLTLR 249



>NP_997698
          Length = 2466

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    ENYMITMR 8
            + YMIT+R
Sbjct: 2309 DGYMITVR 2316



>NP_001597
          Length = 2436

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    ENYMITMR 8
            + YMIT+R
Sbjct: 2279 DGYMITVR 2286



>XP_006717059
          Length = 2435

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    ENYMITMR 8
            + YMIT+R
Sbjct: 2278 DGYMITVR 2285



>XP_006715849
          Length = 374

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   ENYMITMR 8
           ++YM+T+R
Sbjct: 230 QDYMLTLR 237



>XP_006715848
          Length = 407

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   ENYMITMR 8
           ++YM+T+R
Sbjct: 263 QDYMLTLR 270



>NP_001006658
          Length = 1181

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   NYMITMRL 9
           N+MI M+L
Sbjct: 188 NFMIKMKL 195



>NP_065830
          Length = 1170

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   NYMITMRL 9
           N+MI M+L
Sbjct: 188 NFMIKMKL 195



>NP_001107604
          Length = 2694

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NYMITM 7
           NY+ITM
Sbjct: 117 NYVITM 122



>XP_005253558
          Length = 897

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   NYMITMR 8
           N+++TMR
Sbjct: 253 NFLLTMR 259



>XP_006719219
          Length = 910

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   NYMITMR 8
           N+++TMR
Sbjct: 322 NFLLTMR 328



>XP_005253556
          Length = 944

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   NYMITMR 8
           N+++TMR
Sbjct: 322 NFLLTMR 328



>XP_005253555
          Length = 966

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   NYMITMR 8
           N+++TMR
Sbjct: 322 NFLLTMR 328



>XP_005253210
          Length = 503

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   ENYMITMRL 9
           E+Y++ +RL
Sbjct: 359 EHYLVLLRL 367



>XP_006712765
          Length = 2679

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NYMITM 7
           NY+ITM
Sbjct: 117 NYVITM 122



>XP_006712764
          Length = 2697

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NYMITM 7
           NY+ITM
Sbjct: 117 NYVITM 122



>XP_005246844
          Length = 2707

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NYMITM 7
           NY+ITM
Sbjct: 117 NYVITM 122



>XP_006712763
          Length = 2716

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NYMITM 7
           NY+ITM
Sbjct: 117 NYVITM 122



>XP_006712762
          Length = 2723

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NYMITM 7
           NY+ITM
Sbjct: 117 NYVITM 122



>XP_006712761
          Length = 2736

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NYMITM 7
           NY+ITM
Sbjct: 117 NYVITM 122



>NP_110410
          Length = 487

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   ENYMITMRL 9
           E+Y++ +RL
Sbjct: 343 EHYLVLLRL 351



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.333    0.138    0.376 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 343,959
Number of extensions: 388
Number of successful extensions: 30
Number of sequences better than 10000.0: 30
Number of HSP's gapped: 30
Number of HSP's successfully gapped: 30
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)