Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004988                                                              16    7355
NP_114161                                                              16    7355
NP_115894                                                              16    7355
XP_006713841                                                           16    7355
XP_005264828                                                           16    7355
XP_005245254                                                           16    7355
XP_005245253                                                           16    7355
NP_878918                                                              15    9605
NP_055995                                                              15    9605
NP_620168                                                              15    9605
NP_001171473                                                           15    9605
XP_006722720                                                           15    9605
XP_006722719                                                           15    9605
XP_006722718                                                           15    9605
XP_006722717                                                           15    9605
XP_006720147                                                           15    9605
XP_005267517                                                           15    9605
XP_005267516                                                           15    9605
XP_005267515                                                           15    9605
XP_005267514                                                           15    9605
XP_005267513                                                           15    9605
XP_005267512                                                           15    9605
XP_005267511                                                           15    9605
>NP_004988
          Length = 477

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   MLFIRSTQ 9
           +LFIRS Q
Sbjct: 230 ILFIRSAQ 237
>NP_114161
          Length = 569

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   FIRSTQ 9
           FIRSTQ
Sbjct: 439 FIRSTQ 444
>NP_115894
          Length = 601

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   LFIRSTQ 9
           LF+R+TQ
Sbjct: 536 LFVRATQ 542
>XP_006713841
          Length = 540

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   FIRSTQ 9
           FIRSTQ
Sbjct: 439 FIRSTQ 444
>XP_005264828
          Length = 524

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   LFIRSTQ 9
           LF+R+TQ
Sbjct: 459 LFVRATQ 465
>XP_005245254
          Length = 315

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  MLFIRSTQ 9
          +LFIRS Q
Sbjct: 68 ILFIRSAQ 75
>XP_005245253
          Length = 477

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   MLFIRSTQ 9
           +LFIRS Q
Sbjct: 230 ILFIRSAQ 237
>NP_878918
          Length = 6907

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    EMLFIRSTQ 9
            +ML ++STQ
Sbjct: 2816 QMLVLKSTQ 2824
>NP_055995
          Length = 6885

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    EMLFIRSTQ 9
            +ML ++STQ
Sbjct: 2816 QMLVLKSTQ 2824
>NP_620168
          Length = 1300

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   MLFIRSTQ 9
           +LFIR+T+
Sbjct: 192 LLFIRATR 199
>NP_001171473
          Length = 1263

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   MLFIRSTQ 9
           +LFIR+T+
Sbjct: 139 LLFIRATR 146
>XP_006722720
          Length = 820

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   MLFIRSTQ 9
           +LFIR+T+
Sbjct: 192 LLFIRATR 199
>XP_006722719
          Length = 1224

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   MLFIRSTQ 9
           +LFIR+T+
Sbjct: 106 LLFIRATR 113
>XP_006722718
          Length = 1310

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   MLFIRSTQ 9
           +LFIR+T+
Sbjct: 192 LLFIRATR 199
>XP_006722717
          Length = 1310

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   MLFIRSTQ 9
           +LFIR+T+
Sbjct: 192 LLFIRATR 199
>XP_006720147
          Length = 4788

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    EMLFIRSTQ 9
            +ML ++STQ
Sbjct: 2816 QMLVLKSTQ 2824
>XP_005267517
          Length = 6883

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    EMLFIRSTQ 9
            +ML ++STQ
Sbjct: 2816 QMLVLKSTQ 2824
>XP_005267516
          Length = 6884

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    EMLFIRSTQ 9
            +ML ++STQ
Sbjct: 2816 QMLVLKSTQ 2824
>XP_005267515
          Length = 6899

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    EMLFIRSTQ 9
            +ML ++STQ
Sbjct: 2816 QMLVLKSTQ 2824
>XP_005267514
          Length = 6908

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    EMLFIRSTQ 9
            +ML ++STQ
Sbjct: 2816 QMLVLKSTQ 2824
>XP_005267513
          Length = 6921

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    EMLFIRSTQ 9
            +ML ++STQ
Sbjct: 2816 QMLVLKSTQ 2824
>XP_005267512
          Length = 6922

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    EMLFIRSTQ 9
            +ML ++STQ
Sbjct: 2816 QMLVLKSTQ 2824
>XP_005267511
          Length = 6922

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    EMLFIRSTQ 9
            +ML ++STQ
Sbjct: 2816 QMLVLKSTQ 2824
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.334    0.137    0.346 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 320,832
Number of extensions: 426
Number of successful extensions: 23
Number of sequences better than 10000.0: 23
Number of HSP's gapped: 23
Number of HSP's successfully gapped: 23
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.4 bits)
S2: 28 (15.4 bits)

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