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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005742                                                              19     665
NP_671714                                                              19     665
XP_006715890                                                           19     665
XP_005250160                                                           19     665
XP_005250159                                                           19     665
XP_006721506                                                           17    2528
XP_005256500                                                           17    2528
XP_006721505                                                           17    2528
XP_006721504                                                           17    2528
NP_998762                                                              17    2528
NP_004963                                                              16    7355
NP_060440                                                              16    7355
XP_005251512                                                           16    7355
NP_000217                                                              15    9605
NP_006218                                                              15    9605
NP_872617                                                              15    9605
NP_001229849                                                           15    9605
NP_055426                                                              15    9605
NP_002266                                                              15    9605
NP_001229850                                                           15    9605
XP_005260496                                                           15    9605
XP_005257402                                                           15    9605
XP_006721954                                                           15    9605
XP_005248757                                                           15    9605
XP_006715468                                                           15    9605
XP_005248756                                                           15    9605
XP_006715467                                                           15    9605

>NP_005742
          Length = 3907

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    EMEVELSSM 9
            EM+VELSSM
Sbjct: 3135 EMQVELSSM 3143



>NP_671714
          Length = 3899

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    EMEVELSSM 9
            EM+VELSSM
Sbjct: 3127 EMQVELSSM 3135



>XP_006715890
          Length = 3860

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    EMEVELSSM 9
            EM+VELSSM
Sbjct: 3088 EMQVELSSM 3096



>XP_005250160
          Length = 3939

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    EMEVELSSM 9
            EM+VELSSM
Sbjct: 3167 EMQVELSSM 3175



>XP_005250159
          Length = 3952

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    EMEVELSSM 9
            EM+VELSSM
Sbjct: 3180 EMQVELSSM 3188



>XP_006721506
          Length = 410

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1  EMEVELSSM 9
          EME+E+S+M
Sbjct: 18 EMEMEISNM 26



>XP_005256500
          Length = 458

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1  EMEVELSSM 9
          EME+E+S+M
Sbjct: 66 EMEMEISNM 74



>XP_006721505
          Length = 492

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   EMEVELSSM 9
           EME+E+S+M
Sbjct: 100 EMEMEISNM 108



>XP_006721504
          Length = 492

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   EMEVELSSM 9
           EME+E+S+M
Sbjct: 100 EMEMEISNM 108



>NP_998762
          Length = 492

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   EMEVELSSM 9
           EME+E+S+M
Sbjct: 100 EMEMEISNM 108



>NP_004963
          Length = 1132

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   MEVELSSM 9
           +E+ELSS+
Sbjct: 352 LEIELSSL 359



>NP_060440
          Length = 1164

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   EMEVELS 7
           E+EVELS
Sbjct: 829 ELEVELS 835



>XP_005251512
          Length = 1132

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   MEVELSSM 9
           +E+ELSS+
Sbjct: 352 LEIELSSL 359



>NP_000217
          Length = 623

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   EMEVELSS 8
           E+E+EL S
Sbjct: 379 ELEIELQS 386



>NP_006218
          Length = 493

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   EVELSSM 9
           EV+LSSM
Sbjct: 361 EVQLSSM 367



>NP_872617
          Length = 441

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   EVELSSM 9
           EV+LSSM
Sbjct: 309 EVQLSSM 315



>NP_001229849
          Length = 398

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   EVELSSM 9
           EV+LSSM
Sbjct: 266 EVQLSSM 272



>NP_055426
          Length = 5596

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    EMEVELSSM 9
            EMEVE+ ++
Sbjct: 5224 EMEVEIQTV 5232



>NP_002266
          Length = 456

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   EMEVELSS 8
           E+E+EL S
Sbjct: 331 ELEIELQS 338



>NP_001229850
          Length = 405

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   EVELSSM 9
           EV+LSSM
Sbjct: 273 EVQLSSM 279



>XP_005260496
          Length = 513

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   EVELSSM 9
           EV+LSSM
Sbjct: 381 EVQLSSM 387



>XP_005257402
          Length = 456

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   EMEVELSS 8
           E+E+EL S
Sbjct: 331 ELEIELQS 338



>XP_006721954
          Length = 480

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   EMEVELSS 8
           E+E+EL S
Sbjct: 331 ELEIELQS 338



>XP_005248757
          Length = 3591

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    EMEVELSSM 9
            EMEVE+ ++
Sbjct: 3219 EMEVEIQTV 3227



>XP_006715468
          Length = 3744

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    EMEVELSSM 9
            EMEVE+ ++
Sbjct: 3372 EMEVEIQTV 3380



>XP_005248756
          Length = 3905

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    EMEVELSSM 9
            EMEVE+ ++
Sbjct: 3533 EMEVEIQTV 3541



>XP_006715467
          Length = 5550

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    EMEVELSSM 9
            EMEVE+ ++
Sbjct: 5224 EMEVEIQTV 5232



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.295    0.109    0.230 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 370,704
Number of extensions: 360
Number of successful extensions: 27
Number of sequences better than 10000.0: 27
Number of HSP's gapped: 27
Number of HSP's successfully gapped: 27
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.1 bits)
S2: 28 (15.4 bits)