Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

• Home Page
• Cancer-specific
• Epitope Search
• Data Retrieval
• Browse Gene
• Browse Tissue
• Sequence Similarity
• Partially Personalized
• Submit a Protein
• Vaccine Target
• Proteome Data
• NGS data
• BLAST & Predict
• Fully Personalized
• Proteins Pair
• Proteome Pair
• Advanced Tools
• Epitope Mapping
• Cross Reactivity
• Important Information
• Acknowledgement
• Algorithm
• Help
• Developers
• Contact

Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005091                                                              18    1135
NP_653080                                                              18    1135
XP_005267292                                                           18    1135
XP_005267291                                                           18    1135
XP_005267290                                                           18    1135
NP_060003                                                              17    4312
XP_005256707                                                           17    4312
NP_060004                                                              16    5631
NP_002463                                                              16    5631
NP_001106970                                                           16    5631
NP_878250                                                              16    5631
NP_001093582                                                           16    5631
XP_006720131                                                           16    5631

>NP_005091
          Length = 1782

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   EMATKSAV 8
           EMATKSAV
Sbjct: 123 EMATKSAV 130



>NP_653080
          Length = 1684

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1  EMATKSAV 8
          EMATKSAV
Sbjct: 25 EMATKSAV 32



>XP_005267292
          Length = 1677

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1  EMATKSAV 8
          EMATKSAV
Sbjct: 18 EMATKSAV 25



>XP_005267291
          Length = 1684

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1  EMATKSAV 8
          EMATKSAV
Sbjct: 25 EMATKSAV 32



>XP_005267290
          Length = 1782

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   EMATKSAV 8
           EMATKSAV
Sbjct: 123 EMATKSAV 130



>NP_060003
          Length = 1939

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   MATKSAVD 9
           MAT SAVD
Sbjct: 333 MATDSAVD 340



>XP_005256707
          Length = 1940

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   MATKSAVD 9
           MAT SAVD
Sbjct: 333 MATDSAVD 340



>NP_060004
          Length = 1941

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   MATKSAVD 9
           MAT SA+D
Sbjct: 333 MATDSAID 340



>NP_002463
          Length = 1937

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   MATKSAVD 9
           MAT SA+D
Sbjct: 333 MATDSAID 340



>NP_001106970
          Length = 956

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   EMATKSAVD 9
           ++ATK++VD
Sbjct: 923 DLATKNSVD 931



>NP_878250
          Length = 727

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   EMATKSAVD 9
           ++ATK++VD
Sbjct: 694 DLATKNSVD 702



>NP_001093582
          Length = 1941

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   MATKSAVD 9
           MAT SA+D
Sbjct: 333 MATDSAID 340



>XP_006720131
          Length = 957

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   EMATKSAVD 9
           ++ATK++VD
Sbjct: 924 DLATKNSVD 932



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.295    0.103    0.235 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 205,832
Number of extensions: 138
Number of successful extensions: 13
Number of sequences better than 10000.0: 13
Number of HSP's gapped: 13
Number of HSP's successfully gapped: 13
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.2 bits)
S2: 28 (15.4 bits)