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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              20     509
NP_001075109                                                           20     509
NP_005413                                                              16    5631
XP_005271710                                                           16    5631
NP_000384                                                              16    7355
NP_002499                                                              16    7355
XP_006711834                                                           16    7355
NP_001933                                                              15    9605
NP_998820                                                              15    9605
NP_001171914                                                           15    9605
NP_775833                                                              15    9605
NP_001273508                                                           15    9605
NP_001273509                                                           15    9605
NP_001265321                                                           15    9605
XP_006715086                                                           15    9605
XP_005248997                                                           15    9605

>NP_008835
          Length = 4128

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ELSIGIRGY 9
           ELSI IRGY
Sbjct: 358 ELSIAIRGY 366



>NP_001075109
          Length = 4097

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ELSIGIRGY 9
           ELSI IRGY
Sbjct: 358 ELSIAIRGY 366



>NP_005413
          Length = 2155

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   ELSIGIRGY 9
           ELS+ + GY
Sbjct: 380 ELSVEVNGY 388



>XP_005271710
          Length = 2150

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   ELSIGIRGY 9
           ELS+ + GY
Sbjct: 380 ELSVEVNGY 388



>NP_000384
          Length = 1499

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   SIGIRG 8
           SIGIRG
Sbjct: 604 SIGIRG 609



>NP_002499
          Length = 1247

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   ELSIGIRG 8
           E SIG RG
Sbjct: 696 ECSIGFRG 703



>XP_006711834
          Length = 1156

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   ELSIGIRG 8
           E SIG RG
Sbjct: 696 ECSIGFRG 703



>NP_001933
          Length = 1049

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   ELSIGIR 7
           +LSIG+R
Sbjct: 346 QLSIGVR 352



>NP_998820
          Length = 2634

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 3    SIGIRGY 9
            S+G+ GY
Sbjct: 1093 SVGLHGY 1099



>NP_001171914
          Length = 2642

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 3    SIGIRGY 9
            S+G+ GY
Sbjct: 1008 SVGLHGY 1014



>NP_775833
          Length = 419

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   LSIGIRGY 9
           + IGI GY
Sbjct: 317 IRIGIEGY 324



>NP_001273508
          Length = 253

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   LSIGIRGY 9
           + IGI GY
Sbjct: 151 IRIGIEGY 158



>NP_001273509
          Length = 274

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   LSIGIRGY 9
           + IGI GY
Sbjct: 172 IRIGIEGY 179



>NP_001265321
          Length = 374

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3  SIGIRGY 9
          ++GI+GY
Sbjct: 37 TLGIKGY 43



>XP_006715086
          Length = 419

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   LSIGIRGY 9
           + IGI GY
Sbjct: 317 IRIGIEGY 324



>XP_005248997
          Length = 419

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   LSIGIRGY 9
           + IGI GY
Sbjct: 317 IRIGIEGY 324



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.322    0.154    0.435 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 426,887
Number of extensions: 562
Number of successful extensions: 16
Number of sequences better than 10000.0: 16
Number of HSP's gapped: 16
Number of HSP's successfully gapped: 16
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)