Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001135758                                                           17    4312
NP_078900                                                              17    4312
XP_005262685                                                           17    4312
NP_776168                                                              16    5631
NP_001124429                                                           16    5631
NP_000632                                                              16    5631
NP_996801                                                              16    5631
XP_006720106                                                           16    5631
NP_061720                                                              16    5631
NP_001008844                                                           16    7355
NP_004406                                                              16    7355
NP_116167                                                              16    7355
NP_694822                                                              16    7355
NP_001035998                                                           16    7355
NP_001248758                                                           16    7355
NP_001273479                                                           16    7355
NP_004791                                                              16    7355
NP_001245240                                                           16    7355
NP_997292                                                              16    7355
XP_005247895                                                           16    7355
NP_775837                                                              15    9605
NP_001275677                                                           15    9605
NP_473362                                                              15    9605
NP_001041677                                                           15    9605
XP_006724284                                                           15    9605
XP_006724283                                                           15    9605
XP_006724282                                                           15    9605
XP_005261537                                                           15    9605
XP_005258567                                                           15    9605
NP_789780                                                              15    9605
>NP_001135758
          Length = 1091

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    ILSLWS 9
            ILSLWS
Sbjct: 1000 ILSLWS 1005
>NP_078900
          Length = 1091

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    ILSLWS 9
            ILSLWS
Sbjct: 1000 ILSLWS 1005
>XP_005262685
          Length = 1113

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    ILSLWS 9
            ILSLWS
Sbjct: 1022 ILSLWS 1027
>NP_776168
          Length = 832

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  ELAILSLW 8
          E+A+L LW
Sbjct: 5  EVAVLLLW 12
>NP_001124429
          Length = 951

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  ELAILSLW 8
          E+A+L LW
Sbjct: 5  EVAVLLLW 12
>NP_000632
          Length = 199

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  LAILSLW 8
          L +LSLW
Sbjct: 9  LVVLSLW 15
>NP_996801
          Length = 323

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   ELAILSLW 8
           +LAI +LW
Sbjct: 197 DLAIFALW 204
>XP_006720106
          Length = 287

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   ELAILSLW 8
           +LAI +LW
Sbjct: 161 DLAIFALW 168
>NP_061720
          Length = 4024

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   LAILSLW 8
           LA+L LW
Sbjct: 279 LAVLDLW 285
>NP_001008844
          Length = 2272

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   AILSLWS 9
           AIL+LW+
Sbjct: 532 AILALWN 538
>NP_004406
          Length = 2871

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   AILSLWS 9
           AIL+LW+
Sbjct: 532 AILALWN 538
>NP_116167
          Length = 464

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   ELAILSLWS 9
           E  +LSLW+
Sbjct: 449 ESLVLSLWT 457
>NP_694822
          Length = 465

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   ELAILSLWS 9
           E  +LSLW+
Sbjct: 450 ESLVLSLWT 458
>NP_001035998
          Length = 465

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   ELAILSLWS 9
           E  +LSLW+
Sbjct: 450 ESLVLSLWT 458
>NP_001248758
          Length = 464

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   ELAILSLWS 9
           E  +LSLW+
Sbjct: 449 ESLVLSLWT 457
>NP_001273479
          Length = 153

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  AILSLWS 9
          A+L LWS
Sbjct: 15 AVLPLWS 21
>NP_004791
          Length = 663

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   LAILSLW 8
           +AIL+LW
Sbjct: 471 VAILALW 477
>NP_001245240
          Length = 524

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   AILSLWS 9
           ++LS+WS
Sbjct: 184 SVLSIWS 190
>NP_997292
          Length = 100

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  AILSLWS 9
          A+L LWS
Sbjct: 15 AVLPLWS 21
>XP_005247895
          Length = 465

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   ELAILSLWS 9
           E  +LSLW+
Sbjct: 450 ESLVLSLWT 458
>NP_775837
          Length = 2151

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 4    ILSLWS 9
            +LS+WS
Sbjct: 1884 VLSIWS 1889
>NP_001275677
          Length = 604

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   LAILSLW 8
           LA+LS W
Sbjct: 509 LAVLSWW 515
>NP_473362
          Length = 508

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   LAILSLW 8
           LA+L++W
Sbjct: 418 LAVLAVW 424
>NP_001041677
          Length = 247

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   ILSLWS 9
           ILSLW+
Sbjct: 129 ILSLWT 134
>XP_006724284
          Length = 1989

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 4    ILSLWS 9
            +LS+WS
Sbjct: 1722 VLSIWS 1727
>XP_006724283
          Length = 2151

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 4    ILSLWS 9
            +LS+WS
Sbjct: 1884 VLSIWS 1889
>XP_006724282
          Length = 2151

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 4    ILSLWS 9
            +LS+WS
Sbjct: 1884 VLSIWS 1889
>XP_005261537
          Length = 2151

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 4    ILSLWS 9
            +LS+WS
Sbjct: 1884 VLSIWS 1889
>XP_005258567
          Length = 1036

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   LAILSLWS 9
           LAI  LWS
Sbjct: 486 LAIEGLWS 493
>NP_789780
          Length = 1043

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   LAILSLWS 9
           LAI  LWS
Sbjct: 486 LAIEGLWS 493
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.320    0.130    0.414 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 493,825
Number of extensions: 475
Number of successful extensions: 30
Number of sequences better than 10000.0: 30
Number of HSP's gapped: 30
Number of HSP's successfully gapped: 30
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)

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