Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_060101                                                              18    1135
NP_115695                                                              17    3301
NP_001012985                                                           17    4312
NP_001009182                                                           17    4312
NP_003607                                                              17    4312
XP_005263404                                                           17    4312
XP_005263403                                                           17    4312
XP_006714474                                                           17    4312
XP_006714473                                                           17    4312
XP_006714472                                                           17    4312
XP_005263402                                                           17    4312
XP_005263401                                                           17    4312
XP_005263399                                                           17    4312
XP_005263400                                                           17    4312
XP_006714471                                                           17    4312
XP_005263398                                                           17    4312
NP_001866                                                              16    5631
NP_001116105                                                           16    5631
NP_848613                                                              16    5631
NP_997296                                                              16    5631
XP_005256671                                                           16    5631
NP_002105                                                              16    7355
NP_001243000                                                           16    7355
NP_001138508                                                           16    7355
NP_031383                                                              16    7355
NP_060496                                                              16    7355
NP_079544                                                              16    7355
XP_005257603                                                           16    7355
XP_006722058                                                           16    7355
XP_005257602                                                           16    7355
XP_006720324                                                           16    7355
XP_005268158                                                           16    7355
XP_005249099                                                           16    7355
NP_000739                                                              15    9605
NP_003142                                                              15    9605
NP_001230764                                                           15    9605
NP_001027452                                                           15    9605
NP_000915                                                              15    9605
NP_003638                                                              15    9605
NP_001138783                                                           15    9605
NP_001257972                                                           15    9605
NP_002657                                                              15    9605
NP_001274168                                                           15    9605
NP_079467                                                              15    9605
NP_001159447                                                           15    9605
NP_001275698                                                           15    9605
NP_001275697                                                           15    9605
XP_005259977                                                           15    9605
XP_005259975                                                           15    9605
XP_005259974                                                           15    9605
XP_005257806                                                           15    9605
XP_006722206                                                           15    9605
XP_005254991                                                           15    9605
XP_006719514                                                           15    9605
XP_005246877                                                           15    9605
XP_006712782                                                           15    9605
XP_005246874                                                           15    9605
XP_005245556                                                           15    9605
XP_006711206                                                           15    9605
>NP_060101
          Length = 1712

 Score = 18.5 bits (36), Expect =  1135
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    EKVPALFLL 9
            EK+PALF L
Sbjct: 1125 EKLPALFFL 1133
>NP_115695
          Length = 188

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   VPALFLL 9
           +PALFLL
Sbjct: 139 IPALFLL 145
>NP_001012985
          Length = 1706

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    EKVPALFLL 9
            +K+PA+F L
Sbjct: 1105 DKLPAIFFL 1113
>NP_001009182
          Length = 265

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   EKVPALFLL 9
           E+VPAL LL
Sbjct: 239 ERVPALNLL 247
>NP_003607
          Length = 280

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   EKVPALFLL 9
           E+VPAL LL
Sbjct: 254 ERVPALNLL 262
>XP_005263404
          Length = 1308

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    EKVPALFLL 9
            +K+PA+F L
Sbjct: 1105 DKLPAIFFL 1113
>XP_005263403
          Length = 1672

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    EKVPALFLL 9
            +K+PA+F L
Sbjct: 1105 DKLPAIFFL 1113
>XP_006714474
          Length = 1674

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    EKVPALFLL 9
            +K+PA+F L
Sbjct: 1073 DKLPAIFFL 1081
>XP_006714473
          Length = 1675

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    EKVPALFLL 9
            +K+PA+F L
Sbjct: 1073 DKLPAIFFL 1081
>XP_006714472
          Length = 1706

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    EKVPALFLL 9
            +K+PA+F L
Sbjct: 1105 DKLPAIFFL 1113
>XP_005263402
          Length = 1707

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    EKVPALFLL 9
            +K+PA+F L
Sbjct: 1105 DKLPAIFFL 1113
>XP_005263401
          Length = 1707

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    EKVPALFLL 9
            +K+PA+F L
Sbjct: 1105 DKLPAIFFL 1113
>XP_005263399
          Length = 1707

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    EKVPALFLL 9
            +K+PA+F L
Sbjct: 1105 DKLPAIFFL 1113
>XP_005263400
          Length = 1707

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    EKVPALFLL 9
            +K+PA+F L
Sbjct: 1105 DKLPAIFFL 1113
>XP_006714471
          Length = 1707

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    EKVPALFLL 9
            +K+PA+F L
Sbjct: 1105 DKLPAIFFL 1113
>XP_005263398
          Length = 1707

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    EKVPALFLL 9
            +K+PA+F L
Sbjct: 1105 DKLPAIFFL 1113
>NP_001866
          Length = 1500

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   EKVPALF 7
           EKVPA++
Sbjct: 156 EKVPAIY 162
>NP_001116105
          Length = 1506

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   EKVPALF 7
           EKVPA++
Sbjct: 162 EKVPAIY 168
>NP_848613
          Length = 508

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   EKVPALFL 8
           E++PAL+L
Sbjct: 417 ERLPALYL 424
>NP_997296
          Length = 129

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  VPALFLL 9
          +PALFLL
Sbjct: 10 LPALFLL 16
>XP_005256671
          Length = 508

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   EKVPALFL 8
           E++PAL+L
Sbjct: 417 ERLPALYL 424
>NP_002105
          Length = 2718

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    EKVPALFLL 9
            +KVP+ F+L
Sbjct: 1628 QKVPSSFML 1636
>NP_001243000
          Length = 5207

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    EKVPALFLL 9
            +KVP LF L
Sbjct: 2048 QKVPVLFHL 2056
>NP_001138508
          Length = 737

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   EKVPALF 7
           EK+P+LF
Sbjct: 326 EKLPSLF 332
>NP_031383
          Length = 738

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   EKVPALF 7
           EK+P+LF
Sbjct: 326 EKLPSLF 332
>NP_060496
          Length = 963

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   EKVPALFL 8
           +KVP +FL
Sbjct: 884 KKVPTIFL 891
>NP_079544
          Length = 319

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   VPALFLL 9
           VP LFLL
Sbjct: 121 VPGLFLL 127
>XP_005257603
          Length = 5256

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    EKVPALFLL 9
            +KVP LF L
Sbjct: 2097 QKVPVLFHL 2105
>XP_006722058
          Length = 5256

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    EKVPALFLL 9
            +KVP LF L
Sbjct: 2097 QKVPVLFHL 2105
>XP_005257602
          Length = 5256

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    EKVPALFLL 9
            +KVP LF L
Sbjct: 2097 QKVPVLFHL 2105
>XP_006720324
          Length = 319

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   VPALFLL 9
           VP LFLL
Sbjct: 121 VPGLFLL 127
>XP_005268158
          Length = 319

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   VPALFLL 9
           VP LFLL
Sbjct: 121 VPGLFLL 127
>XP_005249099
          Length = 2026

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    EKVPALFLL 9
            +KVP+ F+L
Sbjct: 1628 QKVPSSFML 1636
>NP_000739
          Length = 502

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   EKVPALFLL 9
           EK+PAL  +
Sbjct: 341 EKLPALLFM 349
>NP_003142
          Length = 748

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   EKVPALFLL 9
           +K+P  FLL
Sbjct: 589 DKMPGTFLL 597
>NP_001230764
          Length = 748

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   EKVPALFLL 9
           +K+P  FLL
Sbjct: 589 DKMPGTFLL 597
>NP_001027452
          Length = 251

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   KVPALFL 8
           KVP+LF+
Sbjct: 204 KVPSLFV 210
>NP_000915
          Length = 536

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   KVPALFLL 9
           K+P +FL+
Sbjct: 198 KIPTVFLM 205
>NP_003638
          Length = 567

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   EKVPALF 7
           EK P+LF
Sbjct: 391 EKAPSLF 397
>NP_001138783
          Length = 522

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   EKVPAL 6
           EK+PAL
Sbjct: 100 EKIPAL 105
>NP_001257972
          Length = 510

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   KVPALFLL 9
           KVP  FLL
Sbjct: 434 KVPGSFLL 441
>NP_002657
          Length = 522

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   EKVPAL 6
           EK+PAL
Sbjct: 100 EKIPAL 105
>NP_001274168
          Length = 306

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KVPALFLL 9
           K P+LF+L
Sbjct: 266 KFPSLFIL 273
>NP_079467
          Length = 932

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   KVPALFL 8
           K+P LFL
Sbjct: 761 KIPMLFL 767
>NP_001159447
          Length = 516

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   KVPALFLL 9
           K+P +FL+
Sbjct: 178 KIPTVFLM 185
>NP_001275698
          Length = 495

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   KVPALFLL 9
           K+P +FL+
Sbjct: 157 KIPTVFLM 164
>NP_001275697
          Length = 399

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2  KVPALFLL 9
          K+P +FL+
Sbjct: 61 KIPTVFLM 68
>XP_005259977
          Length = 494

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   KVPALFLL 9
           KVP  FLL
Sbjct: 418 KVPGSFLL 425
>XP_005259975
          Length = 502

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   KVPALFLL 9
           KVP  FLL
Sbjct: 426 KVPGSFLL 433
>XP_005259974
          Length = 520

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   KVPALFLL 9
           KVP  FLL
Sbjct: 444 KVPGSFLL 451
>XP_005257806
          Length = 409

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   EKVPALF 7
           EK P+LF
Sbjct: 391 EKAPSLF 397
>XP_006722206
          Length = 434

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   EKVPALF 7
           EK P+LF
Sbjct: 391 EKAPSLF 397
>XP_005254991
          Length = 522

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   EKVPAL 6
           EK+PAL
Sbjct: 100 EKIPAL 105
>XP_006719514
          Length = 399

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2  KVPALFLL 9
          K+P +FL+
Sbjct: 61 KIPTVFLM 68
>XP_005246877
          Length = 251

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   KVPALFL 8
           KVP+LF+
Sbjct: 204 KVPSLFV 210
>XP_006712782
          Length = 748

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   EKVPALFLL 9
           +K+P  FLL
Sbjct: 589 DKMPGTFLL 597
>XP_005246874
          Length = 757

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   EKVPALFLL 9
           +K+P  FLL
Sbjct: 598 DKMPGTFLL 606
>XP_005245556
          Length = 933

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   KVPALFL 8
           K+P LFL
Sbjct: 762 KIPMLFL 768
>XP_006711206
          Length = 502

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   EKVPALFLL 9
           EK+PAL  +
Sbjct: 341 EKLPALLFM 349
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.331    0.151    0.421 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 665,949
Number of extensions: 1062
Number of successful extensions: 59
Number of sequences better than 10000.0: 59
Number of HSP's gapped: 59
Number of HSP's successfully gapped: 59
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)

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