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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005742                                                              18    1935
NP_671714                                                              18    1935
XP_006715890                                                           18    1935
XP_005250160                                                           18    1935
XP_005250159                                                           18    1935
XP_006723106                                                           16    7355
XP_006723105                                                           16    7355
NP_149024                                                              15    9605
NP_001122074                                                           15    9605
NP_005730                                                              15    9605
XP_005254171                                                           15    9605

>NP_005742
          Length = 3907

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    EKVAAAFVS 9
            EKVAAA VS
Sbjct: 2549 EKVAAALVS 2557



>NP_671714
          Length = 3899

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    EKVAAAFVS 9
            EKVAAA VS
Sbjct: 2541 EKVAAALVS 2549



>XP_006715890
          Length = 3860

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    EKVAAAFVS 9
            EKVAAA VS
Sbjct: 2502 EKVAAALVS 2510



>XP_005250160
          Length = 3939

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    EKVAAAFVS 9
            EKVAAA VS
Sbjct: 2581 EKVAAALVS 2589



>XP_005250159
          Length = 3952

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    EKVAAAFVS 9
            EKVAAA VS
Sbjct: 2594 EKVAAALVS 2602



>XP_006723106
          Length = 971

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  EKVAAAFVS 9
          EK+AA+F S
Sbjct: 33 EKMAASFFS 41



>XP_006723105
          Length = 1028

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  EKVAAAFVS 9
          EK+AA+F S
Sbjct: 33 EKMAASFFS 41



>NP_149024
          Length = 159

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  EKVAAAFVS 9
          EK+ AA++S
Sbjct: 37 EKIKAAYLS 45



>NP_001122074
          Length = 762

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   EKVAAAF 7
           EK+AA+F
Sbjct: 461 EKIAASF 467



>NP_005730
          Length = 797

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   EKVAAAF 7
           EK+AA+F
Sbjct: 496 EKIAASF 502



>XP_005254171
          Length = 746

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   EKVAAAF 7
           EK+AA+F
Sbjct: 496 EKIAASF 502



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.314    0.117    0.273 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 300,366
Number of extensions: 453
Number of successful extensions: 11
Number of sequences better than 10000.0: 11
Number of HSP's gapped: 11
Number of HSP's successfully gapped: 11
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)