Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_542166                                                              17    3301
NP_056357                                                              17    3301
NP_001005234                                                           17    3301
NP_001245135                                                           15    9605
NP_001245136                                                           15    9605
NP_005419                                                              15    9605
NP_003451                                                              15    9605
NP_001268451                                                           15    9605
NP_001268449                                                           15    9605
NP_877954                                                              15    9605
NP_001268450                                                           15    9605
NP_115874                                                              15    9605
NP_001277033                                                           15    9605
NP_001276091                                                           15    9605
NP_005086                                                              15    9605
XP_006721364                                                           15    9605
XP_005255619                                                           15    9605
XP_005271388                                                           15    9605
XP_006711098                                                           15    9605
XP_006711097                                                           15    9605
XP_005271387                                                           15    9605
XP_005271385                                                           15    9605
NP_001276092                                                           15    9605
NP_115645                                                              15    9605
>NP_542166
          Length = 1272

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  EKRTISSAE 9
          E+RT+SS E
Sbjct: 27 ERRTVSSKE 35
>NP_056357
          Length = 1272

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  EKRTISSAE 9
          E+RT+SS E
Sbjct: 27 ERRTVSSKE 35
>NP_001005234
          Length = 324

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  EKRTISSAE 9
          EK+TIS AE
Sbjct: 88 EKKTISYAE 96
>NP_001245135
          Length = 823

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   EKRTIS 6
           EKRTIS
Sbjct: 747 EKRTIS 752
>NP_001245136
          Length = 813

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   EKRTIS 6
           EKRTIS
Sbjct: 737 EKRTIS 742
>NP_005419
          Length = 845

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   EKRTIS 6
           EKRTIS
Sbjct: 769 EKRTIS 774
>NP_003451
          Length = 745

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   EKRTISS 7
           EKRT+S+
Sbjct: 738 EKRTVSN 744
>NP_001268451
          Length = 793

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   EKRTISSA 8
           ++RTISSA
Sbjct: 569 KQRTISSA 576
>NP_001268449
          Length = 840

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   EKRTISSA 8
           ++RTISSA
Sbjct: 616 KQRTISSA 623
>NP_877954
          Length = 824

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   EKRTISSA 8
           ++RTISSA
Sbjct: 600 KQRTISSA 607
>NP_001268450
          Length = 880

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   EKRTISSA 8
           ++RTISSA
Sbjct: 656 KQRTISSA 663
>NP_115874
          Length = 840

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   EKRTISSA 8
           ++RTISSA
Sbjct: 616 KQRTISSA 623
>NP_001277033
          Length = 736

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   EKRTISS 7
           EKRT+S+
Sbjct: 729 EKRTVSN 735
>NP_001276091
          Length = 874

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   EKRTISSAE 9
           +K T+SSAE
Sbjct: 571 KKLTLSSAE 579
>NP_005086
          Length = 1548

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    EKRTISSAE 9
            EK+T+S  E
Sbjct: 1059 EKKTLSQGE 1067
>XP_006721364
          Length = 831

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   EKRTISSAE 9
           +K T+SSAE
Sbjct: 528 KKLTLSSAE 536
>XP_005255619
          Length = 834

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   EKRTISS 7
           EKRT+S+
Sbjct: 827 EKRTVSN 833
>XP_005271388
          Length = 1224

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   EKRTISSAE 9
           EK+T+S  E
Sbjct: 735 EKKTLSQGE 743
>XP_006711098
          Length = 1516

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    EKRTISSAE 9
            EK+T+S  E
Sbjct: 1027 EKKTLSQGE 1035
>XP_006711097
          Length = 1542

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    EKRTISSAE 9
            EK+T+S  E
Sbjct: 1053 EKKTLSQGE 1061
>XP_005271387
          Length = 1545

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    EKRTISSAE 9
            EK+T+S  E
Sbjct: 1056 EKKTLSQGE 1064
>XP_005271385
          Length = 1551

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    EKRTISSAE 9
            EK+T+S  E
Sbjct: 1062 EKKTLSQGE 1070
>NP_001276092
          Length = 809

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   EKRTISSAE 9
           +K T+SSAE
Sbjct: 506 KKLTLSSAE 514
>NP_115645
          Length = 874

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   EKRTISSAE 9
           +K T+SSAE
Sbjct: 571 KKLTLSSAE 579
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.286    0.102    0.218 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 347,563
Number of extensions: 307
Number of successful extensions: 24
Number of sequences better than 10000.0: 24
Number of HSP's gapped: 24
Number of HSP's successfully gapped: 24
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (14.8 bits)
S2: 28 (15.4 bits)

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