Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001245210                                                           19     665
NP_000242                                                              19     665
XP_005264389                                                           19     665
NP_056227                                                              17    4312
NP_001107859                                                           17    4312
NP_872412                                                              17    4312
XP_006714269                                                           17    4312
XP_005247335                                                           17    4312
XP_005247333                                                           17    4312
XP_005247330                                                           17    4312
NP_060719                                                              16    5631
NP_001258968                                                           16    5631
NP_001011649                                                           16    5631
NP_060988                                                              16    5631
NP_877428                                                              16    5631
XP_006717248                                                           16    5631
XP_006717247                                                           16    5631
XP_006717246                                                           16    5631
XP_006717245                                                           16    5631
XP_006717244                                                           16    5631
XP_006717243                                                           16    5631
XP_006717242                                                           16    5631
NP_660356                                                              16    7355
NP_659417                                                              16    7355
NP_001269791                                                           16    7355
XP_005271866                                                           16    7355
XP_005271865                                                           16    7355
XP_006715416                                                           16    7355
XP_006715415                                                           16    7355
XP_005266895                                                           16    7355
NP_000375                                                              15    9605
NP_001258691                                                           15    9605
NP_001258692                                                           15    9605
NP_694955                                                              15    9605
NP_001159698                                                           15    9605
NP_060273                                                              15    9605
NP_112202                                                              15    9605
NP_689478                                                              15    9605
NP_001230696                                                           15    9605
XP_005271958                                                           15    9605
>NP_001245210
          Length = 868

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   EKKIQSTLI 9
           EKK+QSTLI
Sbjct: 423 EKKMQSTLI 431
>NP_000242
          Length = 934

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   EKKIQSTLI 9
           EKK+QSTLI
Sbjct: 489 EKKMQSTLI 497
>XP_005264389
          Length = 921

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   EKKIQSTLI 9
           EKK+QSTLI
Sbjct: 489 EKKMQSTLI 497
>NP_056227
          Length = 567

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   EKKIQSTLI 9
           ++K+QSTL+
Sbjct: 458 QRKLQSTLL 466
>NP_001107859
          Length = 418

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   EKKIQSTL 8
           EKKIQS L
Sbjct: 133 EKKIQSIL 140
>NP_872412
          Length = 421

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   EKKIQSTL 8
           EKKIQS L
Sbjct: 136 EKKIQSIL 143
>XP_006714269
          Length = 387

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   EKKIQSTL 8
           EKKIQS L
Sbjct: 115 EKKIQSIL 122
>XP_005247335
          Length = 400

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   EKKIQSTLI 9
           ++K+QSTL+
Sbjct: 291 QRKLQSTLL 299
>XP_005247333
          Length = 431

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   EKKIQSTLI 9
           ++K+QSTL+
Sbjct: 322 QRKLQSTLL 330
>XP_005247330
          Length = 497

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   EKKIQSTLI 9
           ++K+QSTL+
Sbjct: 388 QRKLQSTLL 396
>NP_060719
          Length = 1893

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    EKKIQSTL 8
            EKK+Q+T+
Sbjct: 1836 EKKLQNTM 1843
>NP_001258968
          Length = 1663

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    EKKIQSTL 8
            EKK+Q+T+
Sbjct: 1606 EKKLQNTM 1613
>NP_001011649
          Length = 1814

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    EKKIQSTL 8
            EKK+Q+T+
Sbjct: 1757 EKKLQNTM 1764
>NP_060988
          Length = 874

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   EKKIQSTL 8
           +KKIQ+TL
Sbjct: 479 KKKIQTTL 486
>NP_877428
          Length = 875

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   EKKIQSTL 8
           +KKIQ+TL
Sbjct: 480 KKKIQTTL 487
>XP_006717248
          Length = 1664

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    EKKIQSTL 8
            EKK+Q+T+
Sbjct: 1607 EKKLQNTM 1614
>XP_006717247
          Length = 1670

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    EKKIQSTL 8
            EKK+Q+T+
Sbjct: 1607 EKKLQNTM 1614
>XP_006717246
          Length = 1820

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    EKKIQSTL 8
            EKK+Q+T+
Sbjct: 1757 EKKLQNTM 1764
>XP_006717245
          Length = 1861

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    EKKIQSTL 8
            EKK+Q+T+
Sbjct: 1804 EKKLQNTM 1811
>XP_006717244
          Length = 1867

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    EKKIQSTL 8
            EKK+Q+T+
Sbjct: 1804 EKKLQNTM 1811
>XP_006717243
          Length = 1889

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    EKKIQSTL 8
            EKK+Q+T+
Sbjct: 1826 EKKLQNTM 1833
>XP_006717242
          Length = 1898

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    EKKIQSTL 8
            EKK+Q+T+
Sbjct: 1835 EKKLQNTM 1842
>NP_660356
          Length = 481

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   EKKIQSTLI 9
           +KKIQS L+
Sbjct: 433 DKKIQSYLL 441
>NP_659417
          Length = 469

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   EKKIQSTLI 9
           EK+I++TL+
Sbjct: 410 EKEIKTTLV 418
>NP_001269791
          Length = 489

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   EKKIQSTLI 9
           +KKIQS L+
Sbjct: 441 DKKIQSYLL 449
>XP_005271866
          Length = 401

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   EKKIQSTLI 9
           +KKIQS L+
Sbjct: 353 DKKIQSYLL 361
>XP_005271865
          Length = 481

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   EKKIQSTLI 9
           +KKIQS L+
Sbjct: 433 DKKIQSYLL 441
>XP_006715416
          Length = 501

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   EKKIQSTLI 9
           EK+I++TL+
Sbjct: 410 EKEIKTTLV 418
>XP_006715415
          Length = 501

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   EKKIQSTLI 9
           EK+I++TL+
Sbjct: 410 EKEIKTTLV 418
>XP_005266895
          Length = 501

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   EKKIQSTLI 9
           EK+I++TL+
Sbjct: 410 EKEIKTTLV 418
>NP_000375
          Length = 4563

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    KKIQSTLI 9
            KK+QST +
Sbjct: 4540 KKLQSTTV 4547
>NP_001258691
          Length = 493

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   EKKIQSTL 8
           E+K QSTL
Sbjct: 407 EQKYQSTL 414
>NP_001258692
          Length = 458

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   EKKIQSTL 8
           E+K QSTL
Sbjct: 372 EQKYQSTL 379
>NP_694955
          Length = 986

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EKKIQSTLI 9
           E K+Q TLI
Sbjct: 709 EGKLQKTLI 717
>NP_001159698
          Length = 960

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EKKIQSTLI 9
           E K+Q TLI
Sbjct: 683 EGKLQKTLI 691
>NP_060273
          Length = 493

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   EKKIQSTL 8
           E+K QSTL
Sbjct: 407 EQKYQSTL 414
>NP_112202
          Length = 129

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  EKKIQSTLI 9
          EKK Q TL+
Sbjct: 87 EKKAQLTLL 95
>NP_689478
          Length = 158

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  EKKIQSTL 8
          +KK+QS+L
Sbjct: 36 DKKLQSSL 43
>NP_001230696
          Length = 78

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  EKKIQSTL 8
          +KK+QS+L
Sbjct: 36 DKKLQSSL 43
>XP_005271958
          Length = 941

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EKKIQSTLI 9
           E K+Q TLI
Sbjct: 664 EGKLQKTLI 672
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.306    0.124    0.277 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 438,186
Number of extensions: 565
Number of successful extensions: 40
Number of sequences better than 10000.0: 40
Number of HSP's gapped: 40
Number of HSP's successfully gapped: 40
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.4 bits)
S2: 28 (15.4 bits)

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