Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001268421                                                           20     509
NP_000170                                                              20     509
NP_001268422                                                           20     509
NP_001268423                                                           20     509
XP_005264328                                                           20     509
NP_001549                                                              16    5631
NP_694992                                                              16    5631
NP_055989                                                              16    5631
NP_001035150                                                           16    5631
XP_006718896                                                           16    5631
XP_005247500                                                           16    5631
NP_004780                                                              16    7355
NP_001004334                                                           16    7355
NP_001014434                                                           16    7355
XP_006717386                                                           16    7355
XP_001717126                                                           16    7355
XP_005245407                                                           16    7355
XP_006711523                                                           16    7355
XP_005245406                                                           16    7355
XP_006711522                                                           16    7355
NP_839943                                                              16    7355
NP_064589                                                              16    7355
NP_001128715                                                           15    9605
NP_001128717                                                           15    9605
NP_001128716                                                           15    9605
NP_001008657                                                           15    9605
NP_000347                                                              15    9605
NP_001599                                                              15    9605
NP_001166957                                                           15    9605
NP_001229768                                                           15    9605
NP_001182070                                                           15    9605
NP_056386                                                              15    9605
NP_001152863                                                           15    9605
XP_005276585                                                           15    9605
XP_370934                                                              15    9605
XP_006715520                                                           15    9605
XP_005268566                                                           15    9605
XP_005268565                                                           15    9605
XP_005268564                                                           15    9605
XP_005268563                                                           15    9605
XP_005268562                                                           15    9605
XP_005268561                                                           15    9605
XP_005268560                                                           15    9605
XP_005268559                                                           15    9605
XP_005246574                                                           15    9605
XP_005273161                                                           15    9605
XP_001716413                                                           15    9605
NP_001152862                                                           15    9605
NP_000849                                                              15    9605
NP_001093879                                                           15    9605
>NP_001268421
          Length = 1230

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   EKAQKTGLI 9
           EKA+KTGLI
Sbjct: 789 EKARKTGLI 797
>NP_000170
          Length = 1360

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   EKAQKTGLI 9
           EKA+KTGLI
Sbjct: 919 EKARKTGLI 927
>NP_001268422
          Length = 1058

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   EKAQKTGLI 9
           EKA+KTGLI
Sbjct: 617 EKARKTGLI 625
>NP_001268423
          Length = 1058

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   EKAQKTGLI 9
           EKA+KTGLI
Sbjct: 617 EKARKTGLI 625
>XP_005264328
          Length = 1261

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   EKAQKTGLI 9
           EKA+KTGLI
Sbjct: 820 EKARKTGLI 828
>NP_001549
          Length = 578

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EKAQKTGLI 9
           EKA KTG +
Sbjct: 455 EKATKTGCL 463
>NP_694992
          Length = 239

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  EKAQKTGL 8
          E AQKTG+
Sbjct: 11 ETAQKTGV 18
>NP_055989
          Length = 939

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   EKAQKTGL 8
           E AQKTG+
Sbjct: 299 EAAQKTGV 306
>NP_001035150
          Length = 175

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  KAQKTG 7
          KAQKTG
Sbjct: 69 KAQKTG 74
>XP_006718896
          Length = 558

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EKAQKTGLI 9
           EKA KTG +
Sbjct: 435 EKATKTGCL 443
>XP_005247500
          Length = 152

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  KAQKTG 7
          KAQKTG
Sbjct: 46 KAQKTG 51
>NP_004780
          Length = 406

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   AQKTGL 8
           AQKTGL
Sbjct: 300 AQKTGL 305
>NP_001004334
          Length = 2367

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    EKAQKTGL 8
            E+A KTGL
Sbjct: 1193 ERAGKTGL 1200
>NP_001014434
          Length = 388

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   AQKTGL 8
           AQKTGL
Sbjct: 292 AQKTGL 297
>XP_006717386
          Length = 314

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   AQKTGL 8
           AQKTGL
Sbjct: 300 AQKTGL 305
>XP_001717126
          Length = 1029

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   EKAQKTG 7
           E+AQ+TG
Sbjct: 412 ERAQRTG 418
>XP_005245407
          Length = 330

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   AQKTGL 8
           AQKTGL
Sbjct: 301 AQKTGL 306
>XP_006711523
          Length = 339

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   AQKTGL 8
           AQKTGL
Sbjct: 310 AQKTGL 315
>XP_005245406
          Length = 396

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   AQKTGL 8
           AQKTGL
Sbjct: 300 AQKTGL 305
>XP_006711522
          Length = 406

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   AQKTGL 8
           AQKTGL
Sbjct: 310 AQKTGL 315
>NP_839943
          Length = 1631

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   EKAQKTGLI 9
           +KAQ+ GL+
Sbjct: 344 QKAQELGLV 352
>NP_064589
          Length = 397

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   AQKTGL 8
           AQKTGL
Sbjct: 301 AQKTGL 306
>NP_001128715
          Length = 1488

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   EKAQKTG 7
           EKA KTG
Sbjct: 130 EKAGKTG 136
>NP_001128717
          Length = 1412

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   EKAQKTG 7
           EKA KTG
Sbjct: 130 EKAGKTG 136
>NP_001128716
          Length = 1451

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   EKAQKTG 7
           EKA KTG
Sbjct: 130 EKAGKTG 136
>NP_001008657
          Length = 958

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   EKAQKTG 7
           EKA KTG
Sbjct: 130 EKAGKTG 136
>NP_000347
          Length = 1411

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   EKAQKTG 7
           EKA KTG
Sbjct: 130 EKAGKTG 136
>NP_001599
          Length = 430

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  EKAQKTGLI 9
          EKA K GL+
Sbjct: 91 EKAGKQGLL 99
>NP_001166957
          Length = 82

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  EKAQKTGLI 9
          E  +KTGL+
Sbjct: 52 ENNEKTGLV 60
>NP_001229768
          Length = 197

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   EKAQKTG 7
           +KAQ+TG
Sbjct: 190 KKAQRTG 196
>NP_001182070
          Length = 1450

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   EKAQKTG 7
           EKA KTG
Sbjct: 130 EKAGKTG 136
>NP_056386
          Length = 1112

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   EKAQKTGL 8
           +K+Q TGL
Sbjct: 263 QKSQNTGL 270
>NP_001152863
          Length = 197

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   EKAQKTG 7
           +KAQ+TG
Sbjct: 190 KKAQRTG 196
>XP_005276585
          Length = 741

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KAQKTGLI 9
           KAQ+ GL+
Sbjct: 122 KAQRVGLL 129
>XP_370934
          Length = 741

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KAQKTGLI 9
           KAQ+ GL+
Sbjct: 122 KAQRVGLL 129
>XP_006715520
          Length = 1056

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   EKAQKTGL 8
           +K+Q TGL
Sbjct: 207 QKSQNTGL 214
>XP_005268566
          Length = 994

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   EKAQKTG 7
           EKA KTG
Sbjct: 130 EKAGKTG 136
>XP_005268565
          Length = 995

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   EKAQKTG 7
           EKA KTG
Sbjct: 130 EKAGKTG 136
>XP_005268564
          Length = 1449

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   EKAQKTG 7
           EKA KTG
Sbjct: 130 EKAGKTG 136
>XP_005268563
          Length = 1488

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   EKAQKTG 7
           EKA KTG
Sbjct: 130 EKAGKTG 136
>XP_005268562
          Length = 1489

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   EKAQKTG 7
           EKA KTG
Sbjct: 130 EKAGKTG 136
>XP_005268561
          Length = 1525

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   EKAQKTG 7
           EKA KTG
Sbjct: 130 EKAGKTG 136
>XP_005268560
          Length = 1525

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   EKAQKTG 7
           EKA KTG
Sbjct: 130 EKAGKTG 136
>XP_005268559
          Length = 1526

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   EKAQKTG 7
           EKA KTG
Sbjct: 130 EKAGKTG 136
>XP_005246574
          Length = 409

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  EKAQKTGLI 9
          EKA K GL+
Sbjct: 70 EKAGKQGLL 78
>XP_005273161
          Length = 263

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   EKAQKTG 7
           +KAQ+TG
Sbjct: 256 KKAQRTG 262
>XP_001716413
          Length = 764

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   KAQKTGLI 9
           KAQ+ GL+
Sbjct: 122 KAQRVGLL 129
>NP_001152862
          Length = 218

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   EKAQKTG 7
           +KAQ+TG
Sbjct: 211 KKAQRTG 217
>NP_000849
          Length = 197

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   EKAQKTG 7
           +KAQ+TG
Sbjct: 190 KKAQRTG 196
>NP_001093879
          Length = 1105

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   EKAQKTGL 8
           +K+Q TGL
Sbjct: 256 QKSQNTGL 263
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.304    0.125    0.304 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 451,816
Number of extensions: 731
Number of successful extensions: 50
Number of sequences better than 10000.0: 50
Number of HSP's gapped: 50
Number of HSP's successfully gapped: 50
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.3 bits)
S2: 28 (15.4 bits)

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