Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_722576                                                              20     509
XP_005262424                                                           20     509
NP_002227                                                              17    3301
NP_001080                                                              16    5631
NP_001124438                                                           16    5631
NP_001159726                                                           16    5631
NP_001159725                                                           16    5631
NP_001052                                                              16    5631
NP_001006658                                                           16    7355
NP_065830                                                              16    7355
NP_001164977                                                           16    7355
NP_001164979                                                           16    7355
NP_001164980                                                           16    7355
NP_001164982                                                           16    7355
NP_001164978                                                           16    7355
NP_644808                                                              16    7355
NP_001164981                                                           16    7355
NP_003861                                                              16    7355
XP_006723519                                                           16    7355
XP_006723518                                                           16    7355
XP_006723517                                                           16    7355
NP_839943                                                              16    7355
NP_000238                                                              15    9605
NP_001170990                                                           15    9605
NP_072047                                                              15    9605
NP_001276103                                                           15    9605
NP_001276105                                                           15    9605
NP_001276104                                                           15    9605
NP_653223                                                              15    9605
XP_005257995                                                           15    9605
XP_005257994                                                           15    9605
NP_001273                                                              15    9605
NP_001025177                                                           15    9605
>NP_722576
          Length = 3080

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    EISMTLTHV 9
            EISM LTHV
Sbjct: 2505 EISMNLTHV 2513
>XP_005262424
          Length = 2875

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    EISMTLTHV 9
            EISM LTHV
Sbjct: 2300 EISMNLTHV 2308
>NP_002227
          Length = 494

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   ISMTLTHV 9
           +S+TLTH+
Sbjct: 268 VSLTLTHL 275
>NP_001080
          Length = 1704

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    EISMTLTHV 9
            E+S TL HV
Sbjct: 1240 ELSKTLDHV 1248
>NP_001124438
          Length = 534

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   EISMTLTHV 9
           E+ +TL HV
Sbjct: 489 EVKLTLLHV 497
>NP_001159726
          Length = 466

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   EISMTLTHV 9
           E+ +TL HV
Sbjct: 421 EVKLTLLHV 429
>NP_001159725
          Length = 580

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   EISMTLTHV 9
           E+ +TL HV
Sbjct: 535 EVKLTLLHV 543
>NP_001052
          Length = 534

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   EISMTLTHV 9
           E+ +TL HV
Sbjct: 489 EVKLTLLHV 497
>NP_001006658
          Length = 1181

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   ISMTLTHV 9
           ++MT THV
Sbjct: 434 VAMTKTHV 441
>NP_065830
          Length = 1170

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   ISMTLTHV 9
           ++MT THV
Sbjct: 423 VAMTKTHV 430
>NP_001164977
          Length = 146

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   EISMTLTHV 9
           E+S  +THV
Sbjct: 92  ELSTAITHV 100
>NP_001164979
          Length = 146

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   EISMTLTHV 9
           E+S  +THV
Sbjct: 92  ELSTAITHV 100
>NP_001164980
          Length = 94

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  EISMTLTHV 9
          E+S  +THV
Sbjct: 40 ELSTAITHV 48
>NP_001164982
          Length = 127

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  EISMTLTHV 9
          E+S  +THV
Sbjct: 73 ELSTAITHV 81
>NP_001164978
          Length = 146

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   EISMTLTHV 9
           E+S  +THV
Sbjct: 92  ELSTAITHV 100
>NP_644808
          Length = 146

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   EISMTLTHV 9
           E+S  +THV
Sbjct: 92  ELSTAITHV 100
>NP_001164981
          Length = 94

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  EISMTLTHV 9
          E+S  +THV
Sbjct: 40 ELSTAITHV 48
>NP_003861
          Length = 1657

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    EISMTLTH 8
            E+S+TLT+
Sbjct: 1360 EVSLTLTN 1367
>XP_006723519
          Length = 223

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   ISMTLTH 8
           +SMT TH
Sbjct: 184 LSMTFTH 190
>XP_006723518
          Length = 277

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   ISMTLTH 8
           +SMT TH
Sbjct: 238 LSMTFTH 244
>XP_006723517
          Length = 327

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   ISMTLTH 8
           +SMT TH
Sbjct: 288 LSMTFTH 294
>NP_839943
          Length = 1631

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    EISMTLTH 8
            E+S+TLT+
Sbjct: 1341 EVSLTLTN 1348
>NP_000238
          Length = 383

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   EISMTLTHV 9
           ++ MTL H+
Sbjct: 95  DLRMTLAHI 103
>NP_001170990
          Length = 332

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   EISMTLTHV 9
           ++ MTL H+
Sbjct: 95  DLRMTLAHI 103
>NP_072047
          Length = 723

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   ISMTLTHV 9
           +SMTL H+
Sbjct: 423 LSMTLKHL 430
>NP_001276103
          Length = 656

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   ISMTLTHV 9
           +SMTL H+
Sbjct: 356 LSMTLKHL 363
>NP_001276105
          Length = 534

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   ISMTLTHV 9
           +SMTL H+
Sbjct: 234 LSMTLKHL 241
>NP_001276104
          Length = 598

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   ISMTLTHV 9
           +SMTL H+
Sbjct: 298 LSMTLKHL 305
>NP_653223
          Length = 773

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   EISMTLTH 8
           E +MT TH
Sbjct: 282 EFNMTATH 289
>XP_005257995
          Length = 951

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   EISMTLTH 8
           EIS T TH
Sbjct: 738 EISGTFTH 745
>XP_005257994
          Length = 951

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   EISMTLTH 8
           EIS T TH
Sbjct: 738 EISGTFTH 745
>NP_001273
          Length = 937

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   EISMTLTH 8
           EIS T TH
Sbjct: 724 EISGTFTH 731
>NP_001025177
          Length = 951

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   EISMTLTH 8
           EIS T TH
Sbjct: 738 EISGTFTH 745
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.320    0.123    0.320 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 441,147
Number of extensions: 672
Number of successful extensions: 33
Number of sequences better than 10000.0: 33
Number of HSP's gapped: 33
Number of HSP's successfully gapped: 33
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)

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