Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

• Home Page
• Cancer-specific
• Epitope Search
• Data Retrieval
• Browse Gene
• Browse Tissue
• Sequence Similarity
• Partially Personalized
• Submit a Protein
• Vaccine Target
• Proteome Data
• NGS data
• BLAST & Predict
• Fully Personalized
• Proteins Pair
• Proteome Pair
• Advanced Tools
• Epitope Mapping
• Cross Reactivity
• Important Information
• Acknowledgement
• Algorithm
• Help
• Developers
• Contact

Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005912                                                              18    1482
XP_005248577                                                           18    1482
XP_005248576                                                           18    1482
NP_055230                                                              17    2528
NP_004362                                                              17    4312
NP_001091868                                                           17    4312
NP_001075019                                                           16    5631
XP_006724836                                                           16    5631
XP_005262507                                                           16    5631
XP_006724835                                                           16    5631
XP_006724834                                                           16    5631
XP_006724833                                                           16    5631
XP_006724832                                                           16    5631
XP_006724831                                                           16    5631
XP_006724830                                                           16    5631
XP_005262504                                                           16    5631
NP_001373                                                              16    7355
XP_005271479                                                           16    7355
NP_001193846                                                           15    9605
NP_000716                                                              15    9605
NP_001193844                                                           15    9605
NP_149061                                                              15    9605
NP_001035738                                                           15    9605
NP_001681                                                              15    9605
NP_940885                                                              15    9605
XP_005261294                                                           15    9605
XP_005261293                                                           15    9605
XP_005269199                                                           15    9605
XP_006719654                                                           15    9605
XP_006719653                                                           15    9605
XP_006712516                                                           15    9605
NP_001193845                                                           15    9605
NP_060299                                                              15    9605

>NP_005912
          Length = 1512

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   EIAEAIQL 8
           EIAEAIQL
Sbjct: 864 EIAEAIQL 871



>XP_005248577
          Length = 1349

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   EIAEAIQL 8
           EIAEAIQL
Sbjct: 701 EIAEAIQL 708



>XP_005248576
          Length = 1375

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   EIAEAIQL 8
           EIAEAIQL
Sbjct: 727 EIAEAIQL 734



>NP_055230
          Length = 1053

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    EIAEAIQLV 9
            E+AEAIQ V
Sbjct: 1034 EVAEAIQTV 1042



>NP_004362
          Length = 1224

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    EIAEAIQLV 9
            EIAEA QL+
Sbjct: 1046 EIAEAQQLI 1054



>NP_001091868
          Length = 1233

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    EIAEAIQLV 9
            EIAEA QL+
Sbjct: 1055 EIAEAQQLI 1063



>NP_001075019
          Length = 1593

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   EIAEAIQLV 9
           EIAEA Q+V
Sbjct: 302 EIAEAKQIV 310



>XP_006724836
          Length = 1582

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   EIAEAIQLV 9
           EIAEA Q+V
Sbjct: 302 EIAEAKQIV 310



>XP_005262507
          Length = 1594

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   EIAEAIQLV 9
           EIAEA Q+V
Sbjct: 302 EIAEAKQIV 310



>XP_006724835
          Length = 1615

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   EIAEAIQLV 9
           EIAEA Q+V
Sbjct: 302 EIAEAKQIV 310



>XP_006724834
          Length = 1615

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   EIAEAIQLV 9
           EIAEA Q+V
Sbjct: 302 EIAEAKQIV 310



>XP_006724833
          Length = 1620

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   EIAEAIQLV 9
           EIAEA Q+V
Sbjct: 302 EIAEAKQIV 310



>XP_006724832
          Length = 1640

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   EIAEAIQLV 9
           EIAEA Q+V
Sbjct: 302 EIAEAKQIV 310



>XP_006724831
          Length = 1640

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   EIAEAIQLV 9
           EIAEA Q+V
Sbjct: 302 EIAEAKQIV 310



>XP_006724830
          Length = 1642

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   EIAEAIQLV 9
           EIAEA Q+V
Sbjct: 302 EIAEAKQIV 310



>XP_005262504
          Length = 1642

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   EIAEAIQLV 9
           EIAEA Q+V
Sbjct: 302 EIAEAKQIV 310



>NP_001373
          Length = 408

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   EIAEAIQLV 9
           ++AE++QLV
Sbjct: 335 KVAESLQLV 343



>XP_005271479
          Length = 434

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   EIAEAIQLV 9
           ++AE++QLV
Sbjct: 335 KVAESLQLV 343



>NP_001193846
          Length = 471

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   EIAEAIQLV 9
           E+A+++QLV
Sbjct: 246 ELAKSLQLV 254



>NP_000716
          Length = 484

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   EIAEAIQLV 9
           E+A+++QLV
Sbjct: 259 ELAKSLQLV 267



>NP_001193844
          Length = 443

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   EIAEAIQLV 9
           E+A+++QLV
Sbjct: 218 ELAKSLQLV 226



>NP_149061
          Length = 423

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   EIAEAIQLV 9
           ++ EA+QLV
Sbjct: 406 DVNEAVQLV 414



>NP_001035738
          Length = 71

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  EIAEAIQLV 9
          E+AE +QL+
Sbjct: 2  EVAEKLQLL 10



>NP_001681
          Length = 617

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   EIAEAIQLV 9
           ++AE +QLV
Sbjct: 488 DLAEIVQLV 496



>NP_940885
          Length = 571

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   EIAEAIQL 8
           ++AEA+QL
Sbjct: 113 QLAEALQL 120



>XP_005261294
          Length = 234

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   EIAEAIQLV 9
           ++ EA+QLV
Sbjct: 217 DVNEAVQLV 225



>XP_005261293
          Length = 262

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   EIAEAIQLV 9
           ++ EA+QLV
Sbjct: 245 DVNEAVQLV 253



>XP_005269199
          Length = 308

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1  EIAEAIQLV 9
          E+A+++QLV
Sbjct: 83 ELAKSLQLV 91



>XP_006719654
          Length = 331

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   EIAEAIQLV 9
           E+A+++QLV
Sbjct: 106 ELAKSLQLV 114



>XP_006719653
          Length = 331

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   EIAEAIQLV 9
           E+A+++QLV
Sbjct: 106 ELAKSLQLV 114



>XP_006712516
          Length = 607

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   EIAEAIQL 8
           ++AEA+QL
Sbjct: 113 QLAEALQL 120



>NP_001193845
          Length = 483

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   EIAEAIQLV 9
           E+A+++QLV
Sbjct: 258 ELAKSLQLV 266



>NP_060299
          Length = 393

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   EIAEAIQLV 9
           ++ EA+QLV
Sbjct: 376 DVNEAVQLV 384



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.317    0.133    0.296 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 274,553
Number of extensions: 367
Number of successful extensions: 33
Number of sequences better than 10000.0: 33
Number of HSP's gapped: 33
Number of HSP's successfully gapped: 33
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)