Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_064450                                                              20     299
XP_005273257                                                           20     299
XP_005245315                                                           18    1935
XP_006711447                                                           18    1935
XP_005245314                                                           18    1935
NP_002637                                                              18    1935
NP_003841                                                              17    3301
NP_001231605                                                           16    5631
XP_005248357                                                           16    5631
XP_005248356                                                           16    5631
XP_006712107                                                           16    5631
XP_005264462                                                           16    5631
XP_005264461                                                           16    5631
XP_005264460                                                           16    5631
XP_006712106                                                           16    5631
XP_006712105                                                           16    5631
XP_005264459                                                           16    5631
XP_006712104                                                           16    5631
XP_005264458                                                           16    5631
XP_005264457                                                           16    5631
NP_002176                                                              16    5631
NP_060741                                                              16    5631
NP_001180550                                                           16    7355
NP_001180551                                                           16    7355
NP_001008781                                                           16    7355
NP_060212                                                              16    7355
NP_055900                                                              16    7355
XP_005258988                                                           16    7355
XP_006722557                                                           16    7355
XP_006722556                                                           16    7355
XP_006721540                                                           16    7355
XP_005256600                                                           16    7355
XP_005256599                                                           16    7355
XP_005256598                                                           16    7355
XP_006718827                                                           16    7355
NP_001093868                                                           16    7355
NP_001138732                                                           15    9605
NP_005428                                                              15    9605
NP_001138735                                                           15    9605
NP_001138733                                                           15    9605
NP_001138734                                                           15    9605
NP_689594                                                              15    9605
XP_005248250                                                           15    9605
XP_006712319                                                           15    9605
NP_001186073                                                           15    9605
NP_444270                                                              15    9605
>NP_064450
          Length = 1722

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   EHSVTSAFK 9
           EHSV+SAFK
Sbjct: 842 EHSVSSAFK 850
>XP_005273257
          Length = 1726

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   EHSVTSAFK 9
           EHSV+SAFK
Sbjct: 846 EHSVSSAFK 854
>XP_005245315
          Length = 1607

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    EHSVTSAFK 9
            EH VT +FK
Sbjct: 1117 EHGVTGSFK 1125
>XP_006711447
          Length = 1634

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    EHSVTSAFK 9
            EH VT +FK
Sbjct: 1144 EHGVTGSFK 1152
>XP_005245314
          Length = 1634

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    EHSVTSAFK 9
            EH VT +FK
Sbjct: 1144 EHGVTGSFK 1152
>NP_002637
          Length = 1634

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    EHSVTSAFK 9
            EH VT +FK
Sbjct: 1144 EHGVTGSFK 1152
>NP_003841
          Length = 463

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   HSVTSAFK 9
           H VT AFK
Sbjct: 355 HQVTEAFK 362
>NP_001231605
          Length = 405

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   HSVTSAFK 9
           H VT AFK
Sbjct: 106 HGVTYAFK 113
>XP_005248357
          Length = 261

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  EHSVTSAFK 9
          +HS+T AF+
Sbjct: 52 QHSLTCAFE 60
>XP_005248356
          Length = 262

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  EHSVTSAFK 9
          +HS+T AF+
Sbjct: 52 QHSLTCAFE 60
>XP_006712107
          Length = 290

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   HSVTSAFK 9
           H VT AFK
Sbjct: 106 HGVTYAFK 113
>XP_005264462
          Length = 370

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   HSVTSAFK 9
           H VT AFK
Sbjct: 106 HGVTYAFK 113
>XP_005264461
          Length = 377

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   HSVTSAFK 9
           H VT AFK
Sbjct: 106 HGVTYAFK 113
>XP_005264460
          Length = 384

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   HSVTSAFK 9
           H VT AFK
Sbjct: 106 HGVTYAFK 113
>XP_006712106
          Length = 405

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   HSVTSAFK 9
           H VT AFK
Sbjct: 106 HGVTYAFK 113
>XP_006712105
          Length = 420

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   HSVTSAFK 9
           H VT AFK
Sbjct: 106 HGVTYAFK 113
>XP_005264459
          Length = 421

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   HSVTSAFK 9
           H VT AFK
Sbjct: 106 HGVTYAFK 113
>XP_006712104
          Length = 423

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   HSVTSAFK 9
           H VT AFK
Sbjct: 106 HGVTYAFK 113
>XP_005264458
          Length = 484

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   HSVTSAFK 9
           H VT AFK
Sbjct: 106 HGVTYAFK 113
>XP_005264457
          Length = 484

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   HSVTSAFK 9
           H VT AFK
Sbjct: 106 HGVTYAFK 113
>NP_002176
          Length = 459

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  EHSVTSAFK 9
          +HS+T AF+
Sbjct: 52 QHSLTCAFE 60
>NP_060741
          Length = 484

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   HSVTSAFK 9
           H VT AFK
Sbjct: 106 HGVTYAFK 113
>NP_001180550
          Length = 228

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   EHSVTSAF 8
           +H VTS+F
Sbjct: 117 DHMVTSSF 124
>NP_001180551
          Length = 116

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  EHSVTSAF 8
          +H VTS+F
Sbjct: 5  DHMVTSSF 12
>NP_001008781
          Length = 4557

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    EHSVTSAFK 9
            ++ VTSA+K
Sbjct: 2247 DYEVTSAYK 2255
>NP_060212
          Length = 1178

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   EHSVTSA 7
           EH VTSA
Sbjct: 554 EHCVTSA 560
>NP_055900
          Length = 730

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   EHSVTSAFK 9
           EH V + FK
Sbjct: 253 EHEVNNTFK 261
>XP_005258988
          Length = 116

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  EHSVTSAF 8
          +H VTS+F
Sbjct: 5  DHMVTSSF 12
>XP_006722557
          Length = 1076

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   EHSVTSA 7
           EH VTSA
Sbjct: 452 EHCVTSA 458
>XP_006722556
          Length = 1100

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   EHSVTSA 7
           EH VTSA
Sbjct: 476 EHCVTSA 482
>XP_006721540
          Length = 711

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   EHSVTSAFK 9
           EH V + FK
Sbjct: 234 EHEVNNTFK 242
>XP_005256600
          Length = 727

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   EHSVTSAFK 9
           EH V + FK
Sbjct: 250 EHEVNNTFK 258
>XP_005256599
          Length = 739

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   EHSVTSAFK 9
           EH V + FK
Sbjct: 262 EHEVNNTFK 270
>XP_005256598
          Length = 742

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   EHSVTSAFK 9
           EH V + FK
Sbjct: 265 EHEVNNTFK 273
>XP_006718827
          Length = 3479

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    EHSVTSAFK 9
            ++ VTSA+K
Sbjct: 1137 DYEVTSAYK 1145
>NP_001093868
          Length = 715

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   EHSVTSAFK 9
           EH V + FK
Sbjct: 238 EHEVNNTFK 246
>NP_001138732
          Length = 650

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   HSVTSAF 8
           HS TS+F
Sbjct: 291 HSTTSSF 297
>NP_005428
          Length = 614

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   HSVTSAF 8
           HS TS+F
Sbjct: 275 HSTTSSF 281
>NP_001138735
          Length = 614

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   HSVTSAF 8
           HS TS+F
Sbjct: 275 HSTTSSF 281
>NP_001138733
          Length = 630

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   HSVTSAF 8
           HS TS+F
Sbjct: 291 HSTTSSF 297
>NP_001138734
          Length = 614

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   HSVTSAF 8
           HS TS+F
Sbjct: 275 HSTTSSF 281
>NP_689594
          Length = 3549

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   EHSVTSA 7
           +HSVTS+
Sbjct: 430 DHSVTSS 436
>XP_005248250
          Length = 1074

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   HSVTSAFK 9
           HS+ S FK
Sbjct: 484 HSLASVFK 491
>XP_006712319
          Length = 3308

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   EHSVTSA 7
           +HSVTS+
Sbjct: 189 DHSVTSS 195
>NP_001186073
          Length = 3327

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   EHSVTSA 7
           +HSVTS+
Sbjct: 208 DHSVTSS 214
>NP_444270
          Length = 1074

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   HSVTSAFK 9
           HS+ S FK
Sbjct: 484 HSLASVFK 491
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.307    0.111    0.290 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 423,056
Number of extensions: 594
Number of successful extensions: 46
Number of sequences better than 10000.0: 46
Number of HSP's gapped: 46
Number of HSP's successfully gapped: 46
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)

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