Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004295                                                              23      60
NP_653292                                                              20     509
NP_065716                                                              18    1482
NP_005426                                                              17    4312
NP_057413                                                              17    4312
XP_006726519                                                           17    4312
XP_006716191                                                           17    4312
XP_006716190                                                           17    4312
XP_006713267                                                           17    4312
XP_005265278                                                           17    4312
XP_006713266                                                           17    4312
NP_006784                                                              16    5631
NP_001036182                                                           16    5631
NP_001244947                                                           16    5631
NP_054817                                                              16    5631
NP_149098                                                              16    5631
NP_758962                                                              16    5631
NP_001244946                                                           16    5631
NP_001107558                                                           16    5631
NP_690863                                                              16    5631
NP_277045                                                              16    5631
XP_005276776                                                           16    5631
XP_005262564                                                           16    5631
XP_005265873                                                           16    5631
XP_005265872                                                           16    5631
XP_006710324                                                           16    5631
NP_114114                                                              16    5631
NP_004019                                                              16    7355
NP_001641                                                              16    7355
NP_001642                                                              16    7355
NP_848563                                                              16    7355
NP_066190                                                              16    7355
NP_061874                                                              16    7355
NP_001269786                                                           16    7355
NP_001269788                                                           16    7355
NP_068591                                                              16    7355
XP_005268895                                                           16    7355
XP_006713736                                                           16    7355
XP_005247601                                                           16    7355
XP_006713735                                                           16    7355
XP_006713734                                                           16    7355
NP_002600                                                              15    9605
NP_001138817                                                           15    9605
NP_073153                                                              15    9605
NP_056100                                                              15    9605
NP_443066                                                              15    9605
NP_066288                                                              15    9605
NP_001155907                                                           15    9605
NP_001160                                                              15    9605
NP_940967                                                              15    9605
NP_001138816                                                           15    9605
NP_116227                                                              15    9605
NP_001138815                                                           15    9605
NP_001157908                                                           15    9605
XP_006726446                                                           15    9605
XP_005259413                                                           15    9605
XP_006723511                                                           15    9605
XP_005259411                                                           15    9605
XP_006723510                                                           15    9605
XP_005266731                                                           15    9605
XP_006722494                                                           15    9605
XP_005268521                                                           15    9605
XP_006712754                                                           15    9605
XP_006712753                                                           15    9605
XP_006712221                                                           15    9605
NP_001130005                                                           15    9605
>NP_004295
          Length = 1620

 Score = 22.7 bits (47), Expect =    60
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    EGGHMNMAF 9
            EGGH+NMAF
Sbjct: 1472 EGGHVNMAF 1480
>NP_653292
          Length = 719

 Score = 19.6 bits (39), Expect =   509
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   GGHMNMAF 9
           GGHMN AF
Sbjct: 197 GGHMNEAF 204
>NP_065716
          Length = 222

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   EGGHMNMAF 9
           +GGH+N AF
Sbjct: 204 KGGHINDAF 212
>NP_005426
          Length = 1597

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 2   GGHMN 6
           GGHMN
Sbjct: 867 GGHMN 871
>NP_057413
          Length = 866

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   GGHMNMA 8
           GGH N+A
Sbjct: 652 GGHSNLA 658
>XP_006726519
          Length = 1493

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 2   GGHMN 6
           GGHMN
Sbjct: 848 GGHMN 852
>XP_006716191
          Length = 1543

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 2   GGHMN 6
           GGHMN
Sbjct: 867 GGHMN 871
>XP_006716190
          Length = 1597

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 2   GGHMN 6
           GGHMN
Sbjct: 867 GGHMN 871
>XP_006713267
          Length = 866

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   GGHMNMA 8
           GGH N+A
Sbjct: 652 GGHSNLA 658
>XP_005265278
          Length = 866

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   GGHMNMA 8
           GGH N+A
Sbjct: 652 GGHSNLA 658
>XP_006713266
          Length = 866

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   GGHMNMA 8
           GGH N+A
Sbjct: 652 GGHSNLA 658
>NP_006784
          Length = 256

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   GHMNMA 8
           GHMN+A
Sbjct: 154 GHMNIA 159
>NP_001036182
          Length = 308

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   EGGHMN 6
           EG HMN
Sbjct: 129 EGAHMN 134
>NP_001244947
          Length = 589

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   EGGHMN 6
           EGGH N
Sbjct: 392 EGGHSN 397
>NP_054817
          Length = 238

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   GHMNMA 8
           GHMN+A
Sbjct: 136 GHMNIA 141
>NP_149098
          Length = 387

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   EGGHMN 6
           EG HMN
Sbjct: 208 EGAHMN 213
>NP_758962
          Length = 589

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   EGGHMN 6
           EGGH N
Sbjct: 392 EGGHSN 397
>NP_001244946
          Length = 589

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   EGGHMN 6
           EGGH N
Sbjct: 392 EGGHSN 397
>NP_001107558
          Length = 410

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   EGGHMN 6
           EG HMN
Sbjct: 167 EGAHMN 172
>NP_690863
          Length = 567

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   EGGHMN 6
           EGGH N
Sbjct: 370 EGGHSN 375
>NP_277045
          Length = 1401

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 1   EGGHM 5
           EGGHM
Sbjct: 766 EGGHM 770
>XP_005276776
          Length = 991

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   GHMNMA 8
           GH+NMA
Sbjct: 270 GHINMA 275
>XP_005262564
          Length = 991

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   GHMNMA 8
           GH+NMA
Sbjct: 270 GHINMA 275
>XP_005265873
          Length = 262

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   EGGHMN 6
           EGGH N
Sbjct: 153 EGGHYN 158
>XP_005265872
          Length = 303

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   EGGHMN 6
           EGGH N
Sbjct: 194 EGGHYN 199
>XP_006710324
          Length = 943

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   GHMNMA 8
           GH+NMA
Sbjct: 222 GHINMA 227
>NP_114114
          Length = 330

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   EGGHMN 6
           EGGH N
Sbjct: 221 EGGHYN 226
>NP_004019
          Length = 301

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  GGHMNMA 8
          GGH+N A
Sbjct: 71 GGHINPA 77
>NP_001641
          Length = 323

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  GGHMNMA 8
          GGH+N A
Sbjct: 93 GGHINPA 99
>NP_001642
          Length = 265

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  GGHMNMA 8
          GGH+N A
Sbjct: 65 GGHINPA 71
>NP_848563
          Length = 747

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1   EGGHMN 6
           +GGH+N
Sbjct: 702 KGGHLN 707
>NP_066190
          Length = 295

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  GGHMNMA 8
          GGH+N A
Sbjct: 80 GGHINPA 86
>NP_061874
          Length = 1706

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1   EGGHMNM 7
           E GH+N+
Sbjct: 327 ESGHLNL 333
>NP_001269786
          Length = 1694

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1   EGGHMNM 7
           E GH+N+
Sbjct: 327 ESGHLNL 333
>NP_001269788
          Length = 459

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1   EGGHMNM 7
           E GH+N+
Sbjct: 327 ESGHLNL 333
>NP_068591
          Length = 526

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2   GGHMNMA 8
           GGH++M+
Sbjct: 368 GGHLSMS 374
>XP_005268895
          Length = 222

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  GGHMNMA 8
          GGH+N A
Sbjct: 65 GGHINPA 71
>XP_006713736
          Length = 928

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1   EGGHMNM 7
           E GH+N+
Sbjct: 327 ESGHLNL 333
>XP_005247601
          Length = 1484

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1   EGGHMNM 7
           E GH+N+
Sbjct: 117 ESGHLNL 123
>XP_006713735
          Length = 1656

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1   EGGHMNM 7
           E GH+N+
Sbjct: 327 ESGHLNL 333
>XP_006713734
          Length = 1693

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1   EGGHMNM 7
           E GH+N+
Sbjct: 327 ESGHLNL 333
>NP_002600
          Length = 1106

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   EGGHMNMA 8
           +GG+M+M+
Sbjct: 737 DGGYMDMS 744
>NP_001138817
          Length = 418

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 5/6 (83%)

Query: 1   EGGHMN 6
           +GGH N
Sbjct: 135 QGGHFN 140
>NP_073153
          Length = 1944

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 5/8 (62%)

Query: 2    GGHMNMAF 9
            GG MN  F
Sbjct: 1547 GGEMNYGF 1554
>NP_056100
          Length = 1490

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 2   GGHMNM 7
           GGHM++
Sbjct: 841 GGHMSL 846
>NP_443066
          Length = 1457

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 2   GGHMNM 7
           GGHM++
Sbjct: 841 GGHMSL 846
>NP_066288
          Length = 565

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  EGGHMNM 7
          E GHM+M
Sbjct: 72 EPGHMDM 78
>NP_001155907
          Length = 251

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1   EGGHMNM 7
           E GH+N+
Sbjct: 141 EAGHLNI 147
>NP_001160
          Length = 261

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 2  GGHMNMA 8
          GGH N A
Sbjct: 88 GGHFNPA 94
>NP_940967
          Length = 692

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 4   HMNMAF 9
           H+N+AF
Sbjct: 209 HLNLAF 214
>NP_001138816
          Length = 418

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 5/6 (83%)

Query: 1   EGGHMN 6
           +GGH N
Sbjct: 135 QGGHFN 140
>NP_116227
          Length = 418

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 5/6 (83%)

Query: 1   EGGHMN 6
           +GGH N
Sbjct: 135 QGGHFN 140
>NP_001138815
          Length = 419

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 5/6 (83%)

Query: 1   EGGHMN 6
           +GGH N
Sbjct: 136 QGGHFN 141
>NP_001157908
          Length = 194

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1   EGGHMNM 7
           + GH+NM
Sbjct: 176 QNGHINM 182
>XP_006726446
          Length = 449

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 5/8 (62%)

Query: 2  GGHMNMAF 9
          GG MN  F
Sbjct: 52 GGEMNYGF 59
>XP_005259413
          Length = 314

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 5/6 (83%)

Query: 1  EGGHMN 6
          +GGH N
Sbjct: 31 QGGHFN 36
>XP_006723511
          Length = 314

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 5/6 (83%)

Query: 1  EGGHMN 6
          +GGH N
Sbjct: 31 QGGHFN 36
>XP_005259411
          Length = 386

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 5/6 (83%)

Query: 1   EGGHMN 6
           +GGH N
Sbjct: 103 QGGHFN 108
>XP_006723510
          Length = 419

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 5/6 (83%)

Query: 1   EGGHMN 6
           +GGH N
Sbjct: 136 QGGHFN 141
>XP_005266731
          Length = 982

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 2   GGHMNM 7
           GGHM++
Sbjct: 333 GGHMSL 338
>XP_006722494
          Length = 1490

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 2   GGHMNM 7
           GGHM++
Sbjct: 841 GGHMSL 846
>XP_005268521
          Length = 1042

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   EGGHMNMA 8
           +GG+M+M+
Sbjct: 673 DGGYMDMS 680
>XP_006712754
          Length = 1784

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 5/8 (62%)

Query: 2    GGHMNMAF 9
            GG MN  F
Sbjct: 1387 GGEMNYGF 1394
>XP_006712753
          Length = 1846

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 5/8 (62%)

Query: 2    GGHMNMAF 9
            GG MN  F
Sbjct: 1449 GGEMNYGF 1456
>XP_006712221
          Length = 449

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 5/8 (62%)

Query: 2  GGHMNMAF 9
          GG MN  F
Sbjct: 52 GGEMNYGF 59
>NP_001130005
          Length = 631

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 5/9 (55%)

Query: 1   EGGHMNMAF 9
           E GH N  F
Sbjct: 267 ESGHTNNVF 275
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.324    0.137    0.442 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 726,729
Number of extensions: 946
Number of successful extensions: 66
Number of sequences better than 10000.0: 66
Number of HSP's gapped: 66
Number of HSP's successfully gapped: 66
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)

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